The Enzymatic Hydrolysis of Malathion and Its Inhibition by EPN and Other Organic Phosphorus Compounds

1957 ◽  
Vol 40 (2) ◽  
pp. 664-665
Author(s):  
J W Cook ◽  
Jane R Blake ◽  
Martin W Williams
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Phosphorus ◽  
Phosphorus Compounds ◽  
Hydrolysis Of

scholarly journals Sorption and hydrolysis of added organic phosphorus compounds in lake sediments1

1977 ◽  
Vol 22 (3) ◽  
pp. 415-422 ◽  
Author(s):  
M. G. Rodel ◽  
D. E. Armstrong ◽  
R. F. Harris
Keyword(s):  
Organic Phosphorus ◽  
Phosphorus Compounds ◽  
Hydrolysis Of

scholarly journals Phosphatase Hydrolysis of Organic Phosphorus Compounds

2015 ◽  
Vol 03 (02) ◽  
pp. 39-51 ◽  
Author(s):  
Irenus A. Tazisong ◽  
Zachary N. Senwo ◽  
Zhongqi He
Keyword(s):  
Organic Phosphorus ◽  
Phosphorus Compounds ◽  
Hydrolysis Of

Enzymatic Hydrolysis of Organic Phosphorus in Swine Manure and Soil

2004 ◽  
Vol 33 (1) ◽  
pp. 367-372 ◽  
Author(s):  
Zhongqi He ◽  
Timothy S. Griffin ◽  
C. Wayne Honeycutt
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Phosphorus ◽  
Swine Manure ◽  
Hydrolysis Of

Enzymatic Hydrolysis of Organic Phosphorus in Swine Manure and Soil

2004 ◽  
Vol 33 (1) ◽  
pp. 367 ◽  
Author(s):  
Zhongqi He ◽  
Timothy S. Griffin ◽  
C. Wayne Honeycutt
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Phosphorus ◽  
Swine Manure ◽  
Hydrolysis Of

scholarly journals Study on the Degradation Effect of Three Organophosphorus Hydrolase Mutant on Sarin

2021 ◽  
Vol 271 ◽  
pp. 03054
Author(s):  
Qiamin Gu ◽  
Chuanxin Zhao ◽  
Hailing Xi ◽  
Min Liu ◽  
Gaoyun Chen
Keyword(s):  
Bacillus Subtilis ◽  
Organic Phosphorus ◽  
Organophosphorus Hydrolase ◽  
Phosphorus Compounds ◽  
Hydrolysis Rate ◽  
Recombinant Bacteria ◽  
Recombinant Bacillus Subtilis ◽  
Mutant Genes ◽  
Hydrolysis Of ◽  
Degradation Effect

Organophosphorus hydrolase can effectively degrade organic phosphorus compounds such as sarin. In this study, we constructed a recombinant Bacillus subtilis mutant expressing organophosphorus hydrolase, measured the effect of the mutant on the degradation rate of nerve agent sarin, and selected the optimal mutation scheme. Three different hydrolase mutant genes, 257L, 257Y and 303T, were ligated to PMA0911 vector and transferred into Bacillus subtilis WB800 to construct the target recombinant strain successfully. The recombinant bacteria secreted the target protein by fermentation. The effect of enzyme protein on the degradation of sarin was determined by the benzidine method. The optimal mutant was screened, and its enzymatic performance was explored. The effects of three organophosphorus hydrolase mutants on the hydrolysis rate of sarin were detected. The results showed that the 257Y mutant accelerated the hydrolysis of sarin significantly. Point mutation can improve the enzyme activity of wild-type organophosphorus hydrolase to a certain extent, laying the foundation for subsequent in-depth research.

Sign in / Sign up

Export Citation Format

Share Document