Determination of phosphates in natural and waste waters after photochemical decomposition and acid hydrolysis of organic phosphorus compounds

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

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Cobalt(III) Oxidation Pretreatment of Organic Phosphorus Compounds for a Spectrophotometric Determination of Phosphorus in Water.

Analytical Sciences ◽

10.2116/analsci.10.769 ◽

1994 ◽

Vol 10 (5) ◽

pp. 769-774 ◽

Cited By ~ 4

Author(s):

Hideji TANAKA ◽

Terumi FUKUOKA ◽

Kensaku OKAMOTO

Keyword(s):

Spectrophotometric Determination ◽

Organic Phosphorus ◽

Phosphorus Compounds

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PHOTOCHEMICAL DECOMPOSITION OF ORGANIC PHOSPHORUS COMPOUND INCLUDING POLYPHOSPHATES BY ULTRA-VIOLET RADIATION AND ACID-HYDROLYSIS

Phosphorus Research Bulletin ◽

10.3363/prb1992.3.0_67 ◽

1993 ◽

Vol 3 (0) ◽

pp. 67-72

Author(s):

Jun-ichi ITOH ◽

Masashi KOMATA

Keyword(s):

Acid Hydrolysis ◽

Organic Phosphorus ◽

Ultra Violet ◽

Phosphorus Compound ◽

Ultra Violet Radiation ◽

Photochemical Decomposition ◽

Violet Radiation

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Determination of Unconjugated and Total N-Acetyl-p-aminophenol (NAPA) in Urine and Serum

Clinical Chemistry ◽

10.1093/clinchem/13.3.215 ◽

1967 ◽

Vol 13 (3) ◽

pp. 215-219 ◽

Cited By ~ 4

Author(s):

Karel P M Heirwegh ◽

Johan Fevery

Keyword(s):

Liver Disease ◽

Acid Hydrolysis ◽

Azo Dye ◽

Acidic Medium ◽

Diazonium Salt ◽

Accurate Method ◽

Total N ◽

Hydrolysis Of ◽

Urine And Serum

Abstract A sensitive and accurate method is described for the determination of N-acetyl-p-aminophenol (NAPA) and its metabolites in urine and serum. In strongly acidic medium, p-aminophenol (PAP) resulting from differential extraction and acid hydrolysis of total NAPA and unconjugated NAPA, is diazotized and the diazonium salt coupled with N-(1-naphthyl)ethylenediamine (NED) in the presence of ethanol. The blue azo dye formed is determined spectrophotometrically. Application to liver disease is briefly reported.

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HPTLC determination of diosgenin in fenugreek seeds

Acta Pharmaceutica ◽

10.2478/acph-2018-0002 ◽

2018 ◽

Vol 68 (1) ◽

pp. 97-107 ◽

Cited By ~ 2

Author(s):

Barbara Król-Kogus ◽

Khenifi Mohammed Lamine ◽

Piotr Migas ◽

Messaoud Boudjeniba ◽

Mirosława Krauze-Baranowska

Keyword(s):

Ethyl Acetate ◽

Acid Hydrolysis ◽

Methanolic Extract ◽

Soxhlet Extraction ◽

African Origin ◽

Fenugreek Seeds ◽

Hydrolysis Products ◽

Hydrolysis Of

Abstract A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.

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