Systematic Identification of Antioxidants in Lards, Shortenings, and Vegetable Oils by Thin Layer Chromatography

1981 ◽  
Vol 64 (6) ◽  
pp. 1331-1335
Author(s):  
Carlos H Van Peteghem ◽  
Diana A Dekeyser
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Vegetable Oils ◽  
Corn Oil ◽  
Ascorbyl Palmitate ◽  
Nordihydroguaiaretic Acid ◽  
Rapid Identification ◽  
Butylated Hydroxytoluene ◽  
Systematic Identification ◽  
Layer Chromatography

Abstract A simple and reliable method is described for rapid identification of ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, ethoxyquin, gallates (lauryl, octyl, propyl), nordihydroguaiaretic acid, 3,3’-thiodipropionic acid, tocopherol, t-butylhydroquinone, and 2,4,5-trihydroxybutyrophenone in lards, shortenings, and vegetable oils. The antioxidants are extracted with 95% methanol, concentrated under vacuum at ≤45°C, and analyzed by thin layer chromatography. Three elution solvents, 2 adsorbent types, 2 visualization sprays, and UV viewing at 254 and 366 nm are used. Sunflower and corn oil samples, fortified with 100 ppm antioxidant, were analyzed to establish the validity of the method.

scholarly journals Rapid Identification and Quantitation of Clotrimazole, Miconazole, and Ketokonazole in Pharmaceutical Creams and Ointments by Thin-Layer Chromatography–Densitometry

1996 ◽  
Vol 79 (3) ◽  
pp. 656-660 ◽  
Author(s):  
Utpal Roychowdhury ◽  
Saroj K Das
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Uv Light ◽  
Internal Standard ◽  
Solvent System ◽  
Short Wave ◽  
Rapid Identification ◽  
Pharmaceutical Creams ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract Thin-layer chromatography (TLC)–densitometry was used to separate, identify, and quantitate clotrimazole, miconazole, and ketokonazole (alone or combined with other drugs) in various pharmacopoeial or proprietary creams and ointments. Clotrimazole was extracted from the cream or ointment with ethyl alcohol, and miconazole and ketokonazole were extracted with a mixture of equal volumes of chloroform and isopropyl alcohol. Active ingredients were separated from excipients and other drugs by TLC on a precoated silica gel F254 plate with a solvent system of n-hexane–chloroform–methanol–diethylamine (50 + 40 + 10 + 1, v/v). The 3 azoles were well separated and easily identified in this chromatographic system. The separated azoles were visualized under short-wave UV light and quantitated by scanning densitometry at 220 nm by comparing the integrated areas of samples with those of standard (one azole was used as internal standard for the other). Recoveries from samples spiked with known amounts of azoles were excellent. The method was validated further by comparison with official liquid chromatographic methods.

scholarly journals Rapid Identification and Quantification of Chlorpheniramine Maleate or Pheniramine Maleate in Pharmaceutical Preparations by Thin-Layer Chromatography-Densitometry

2004 ◽  
Vol 87 (6) ◽  
pp. 1319-1322 ◽  
Author(s):  
Seppakkam P Subramaniyan ◽  
Saroj K Das
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Rapid Identification ◽  
Chlorpheniramine Maleate ◽  
Pheniramine Maleate ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Ear Drops

Abstract Thin-layer chromatography (TLC)-densitometry was used to separate, identify, and quantitate chlorpheniramine maleate (CPM) and pheniramine maleate (PM) when present in combination with other drugs in pharmaceutical preparations of tablets, syrups, eye and ear drops, etc. CPM or PM was extracted (tablets, capsules, etc.) or diluted (liquid preparations, if needed) with 80% ethanol and isolated from other ingredients by TLC on silica gel G using cyclohexane–chloroform– methanol–diethylamine (4.5 + 4.0 + 0.5 + 1.0, v/v) as the mobile phase. Separated CPM and PM were detected under shortwave ultraviolet light and quantitated by scanning densitometry at 260 nm. Recoveries of CPM and PM were 100.09 ± 0.77% and 100.09 ± 0.87%, respectively.

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