Liquid Chromatographic Determination of Methocarbamol in Injection and Tablet Dosage Forms: Collaborative Study

1984 ◽  
Vol 67 (2) ◽  
pp. 225-227 ◽  
Author(s):  
Richard L Everett ◽  
◽  
L Allgire ◽  
R J Ballbach ◽  
J Booth ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Dosage Forms ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Tablet Dosage ◽  
Liquid Chromatographic

Abstract A reverse phase liquid chromatographic method was developed for determining methocarbamol in injection and tablet dosage forms. The injections require dilution only; the tablets require a nitration step before introduction into the chromatograph. Response for methocarbamol was linear over the range 0-18 μg, using an ultraviolet detector at 274 nm. Recoveries by the author ranged from 96.1 to 101.9% for authentic injection formulations and 98.0 to 101.0% for authentic tablet formulations. A collaborative study of the method by 6 laboratories resulted in standard deviations of 1.70 and 2.22 for injection and tablet dosage forms, respectively. The method has been adopted official first action.

Liquid Chromatographic Determination of Chlorpropamide in Tablet Dosage Forms: Collaborative Study

1986 ◽  
Vol 69 (3) ◽  
pp. 519-521
Author(s):  
Richard L Everett ◽  
◽  
E Aranda ◽  
M Colon ◽  
J Illuminati ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Dosage Forms ◽  
Commercial Product ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Tablet Dosage ◽  
Liquid Chromatographic

Abstract A reverse-phase liquid chromatographic method was developed for determining chlorpropamide in tablet dosage forms. Linearity was established over the range 0.2-2.0 μg at a wavelength of 240 nm. The Associate Referee obtained a mean recovery for a synthetic tablet mixture of 99.2%, with a relative standard deviation (RSD) of 1.41. For an authentic tablet mixture, collaborators obtained a mean recovery of 99.6% with an RSD of 0.60%. RSDs were 1.24% for 250 mg/tablet commercial product and also for 100 mg/tablet commercial product. The method has been adopted official first action.

Reversed-Phase Liquid Chromatographic Determination of Cromolyn Sodium in Drug Substance and Select Dosage Forms

1994 ◽  
Vol 77 (6) ◽  
pp. 1689-1694 ◽  
Author(s):  
Linda L Ng
Keyword(s):  
Technical Communication ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Liquid Chromatographic Method ◽  
Validation Parameters ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract This study, presented as a technical communication, describes a reversed-phase liquid chromatographic method for select commercial formulations, namely, inhalation solution, nasal solution, capsule and inhalation aerosol. Miscellaneous validation parameters are also discussed.

Liquid Chromatographic Determination of Methyldopa and Methyldopa-Thiazide Combinations in Tablets: Collaborative Study

1984 ◽  
Vol 67 (6) ◽  
pp. 1118-1120
Author(s):  
Ting Susan ◽  
◽  
R L Brown ◽  
L A Dougherty ◽  
J B Schepman ◽  
...  
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Repeatability And Reproducibility ◽  
Liquid Chromatographic

Abstract A reverse phase liquid chromatographic method for the determination of methyldopa, methyldopa-hydrochlorothiazide, and methyldopachlorothiazide in tablets was collaboratively studied by 8 laboratories. Each collaborator received 20 samples that included drug substance, synthetic and commercial tablet compositions. The overall repeatability and reproducibility standard deviations for commercial tablets were 1.11 and 1.75% for methyldopa, 0.96 and 1.62% for chlorothiazide, and 1.21 and 2.15% for hydrochlorothiazide, respectively. The overall recoveries of methyldopa, chlorothiazide, and hydrochlorothiazide added to synthetic tablets were 100.78, 100.70, and 101.34%, respectively. The method has been adopted official first action.

Liquid Chromatographic Determination of Levodopa, Levodopa-Carbidopa, and Related Impurities in Solid Dosage Forms

1986 ◽  
Vol 69 (1) ◽  
pp. 169-173 ◽  
Author(s):  
Susan Ting
Keyword(s):  
Chromatographic Determination ◽  
Dosage Forms ◽  
Solid Dosage Forms ◽  
Reverse Phase ◽  
Solid Dosage ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A rapid reverse phase liquid chromatographic method was developed for the determination of levodopa and levodopa-carbidopa in tablets and capsules. The method also separates these drugs from 3-(3,4,6- trihydroxyphenyl)alanine and methoxytyrosine, impurities of levodopa, and from methyldopa and 3-O-methylcarbidopa, impurities of carbidopa. The mobile phase was 3% acetic acid and the detection wavelength was 280 nm. The method was linear over the concentration range 0.05-0.40 mg levodopa/mL, 0.01-0.06 mg carbidopa/mL, 0.9- 12.8 μg 3-(3,4,6-trihydroxyphenyl)alanine/mL, 0.7-3.1 μg methyldopa/ mL, 5-20 μg methoxytyrosine/mL, and 0.5-3.3 μg 3-O-methylcarbidopa/ mL. Mean recoveries (%) for spiked commercial tablets were: levodopa 100.3, carbidopa 100.4, 3-(3,4,6-trihydroxyphenyl) alanine 99.1, methoxytyrosine 100.0, methyldopa 100.0, and 3-O-methylcarbidopa 99.4.

Liquid Chromatographic Determination of Acetaminophen in Tablets: Collaborative Study

1987 ◽  
Vol 70 (2) ◽  
pp. 212-214
Author(s):  
David J Krieger
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Generic Product ◽  
Single Component ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reverse phase liquid chromatographic method previously reported for the determination of acetaminophen in tablets was collaboratively studied by 5 laboratories. Each collaborator received duplicate samples of a synthetic tablet formulation and 3 powdered commercial tablet composites. The composites represented single-component and multi-component proprietary products and a single-component generic product. The pooled repeatability (CVD„) and reproducibility (CVDJ values for the proprietary tablets were 0.89 and 1.34%, respectively. For the generic tablets, these values were 0.66 and 0.74%, respectively. The pooled recovery value for acetaminophen added to the synthetic formulation was 98.9 ± 0.7% (n = 10) with a CV of 0.75%, CVD„ of 0.37%, and CVD„ of 0.78%. The overall repeatability of the method was 0.64%, and the overall reproducibility was 0.95%. The method has been adopted official first action.

Liquid Chromatographic Determination of Diazepam in Tablets: Collaborative Study

1985 ◽  
Vol 68 (3) ◽  
pp. 545-546
Author(s):  
Michael Tsougros
Keyword(s):  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Photometric Detection ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
The Mean ◽  
Liquid Chromatographic

Abstract A stability indicating liquid chromatographic method for the determination of diazepam in tablets was collaboratively studied by 6 laboratories. The method uses a Cig reverse phase column, a methanolwater mobile phase, p-tolualdehyde as the internal standard, and photometric detection at 254 nm. The collaborators were supplied with a synthetic tablet powder and 3 commercial tablet samples. The mean recovery of diazepam from the synthetic tablet powder was 100.2%. For all samples analyzed, the coefficient of variation was < 1.5%. The method has been adopted official first action.

Liquid Chromatographic Determination of Carminic Acid in Yogurt

1989 ◽  
Vol 72 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Mercedes Jalón ◽  
Majesús Peńa ◽  
Julián C Rivas
Keyword(s):  
Chromatographic Determination ◽  
Carminic Acid ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Array Detection ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reverse-phase liquid chromatographic method is described for the determination of carminic acid in yogurt. A C18 column is used with acetonitrile-1.19M formic acid (19 + 81) as mobile phase and diode array detection. Sample preparation includes deproteinization with papain and purification in a polyamide column. The relative standard deviation for repeated determinations of carminic acid in a commercial strawberry-flavored yogurt was 3.0%. Recoveries of carminic acid added to a natural-flavored yogurt ranged from 87.2 to 95.3% with a mean of 90.2%. The method permits measurement of amounts as low as 0.10 mg/kg.

Liquid Chromatographic Determination of Thiamine in Rodent Feed by Postcolumn Derivatization and Fluorescence Detection

1995 ◽  
Vol 78 (2) ◽  
pp. 307-309 ◽  
Author(s):  
Theresa A Gehring ◽  
Willie M Cooper ◽  
Claude L Holder ◽  
Harold C Thompson
Keyword(s):  
Chromatographic Determination ◽  
Reversed Phase ◽  
Detector Response ◽  
Fluorescence Excitation ◽  
Liquid Chromatographic Method ◽  
Essential Nutrient ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method was developed for determination of the essential nutrient thiamine (vitamin Bi) in rodent feed. Thiamine was extracted with hydrochloric acid, separated by reversed-phase liquid chromatography, derivatized postcolumn to thiochrome with potassium hydroxide and potassium ferricyanide, and detected by fluorescence. Excitation and emission wavelengths were 370 and 430 nm, respectively. Detector response was linear in the range of 2.58 to 15.5 ng of thiamine injected. Instrument detection limit was 5 pg of thiamine injected.

Liquid Chromatographic Determination of Triadimefon in Technical and Formulated Products: Collaborative Study

1985 ◽  
Vol 68 (3) ◽  
pp. 586-589
Author(s):  
Stephen C Slahck
Keyword(s):  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Normal Phase ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Technical Products

Abstract A liquid chromatographic method for the determination of triadimefon (Bayleton™) in triadimefon technical and formulated products has been developed and subjected to a collaborative study with 7 participating collaborators. Formulations were extracted with mobile solvent and analyzed by normal phase chromatography, with 4-chlorophenyl sulfoxide as an internal standard. Collaborators were furnished with standards and samples of technical products, 50% wettable powders, and 25% wettable powders for analysis. Coefficients of variation of the values obtained on these samples were 1.42, 0.82, and 1.05%, respectively. The method has been adopted official first action.

Liquid Chromatographic Determination of Morphine Sulfate and Some Contaminants in Injections and Bulk Drug Material: Collaborative Study

1988 ◽  
Vol 71 (5) ◽  
pp. 1046-1048
Author(s):  
Ada C Bello ◽  
Rita K Jhangiani
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Morphine Sulfate ◽  
System Suitability ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Bulk Drug ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the assay of morphine sulfate and some preservatives and impurities in the bulk drug and in injections has been developed and collaboratively studied in 8 laboratories. Each collaborator analyzed 5 samples: 1 bulk drug, 3 different concentrations of injectable dosages, and 1 prepared mixture containing, in addition to morphine sulfate, phenol, 2-mercaptobenzothiazole, and pseudomorphine. The proposed method quantitates morphine sulfate and resolves the other components for identification using a Clg reverse-phase column with a mobile solvent containing 240 mL methanol, 720 mL 0.005M 1-heptanesulfonic acid Na salt, and 10 mL acetic acid. Samples are prepared by direct dilution with mobile solvent minus 1-heptanesulfonic acid. All collaborators met system suitability requirements and performed the analysis without difficulty. No outliers were found when data were analyzed by the Dixon, Grubbs, double Grubbs, and Cochran tests. Relative standard deviations between laboratories (RSDR) for duplicate determinations of morphine sulfate ranged from 1.4 to 2.1%. Mean morphine sulfate recoveries for the bulk drug and the prepared mixture were 100.8 and 100.4%, respectively. The method has been approved interim official first action.

Sign in / Sign up

Export Citation Format

Share Document