Liquid Chromatographic Determination of Some Amines and Their Amino Acid Precursors in Protein Foods

1988 ◽  
Vol 71 (3) ◽  
pp. 564-568 ◽  
Author(s):  
Frank V Carlucci ◽  
Endel Karmas
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino ◽  
Standard Addition ◽  
Chromatographic Determination ◽  
Liquid Chromatographic Determination ◽  
Amino Acid Precursors ◽  
Liquid Chromatographic

Abstract A quantitative assay has been developed for putrescine, cadaverine, histamine, and several free amino acids—lysine, arginine, and histidine— as a measure of decomposition. The free amines and amino acids are extracted from tissue with methanol. Aliquots of the extracts are passed through ion-exchange columns which are pH-adjusted to retain the cited compounds. After the respective columns are washed, the amino acids and amines are eluted, dansylated, and chromatographed on a suitably prepared LC instrument. The chromatographic responses for the amino acids and amines are compared with their respective standards to determine their concentrations. Validation of the methodology includes standard addition and calibration experiments

Fully automated liquid-chromatographic determination of amino acids.

1988 ◽  
Vol 34 (12) ◽  
pp. 2510-2513 ◽  
Author(s):  
H M van Eijk ◽  
M A van der Heijden ◽  
C L van Berlo ◽  
P B Soeters
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Daily Practice ◽  
Chromatographic Determination ◽  
Precolumn Derivatization ◽  
Inexpensive Method ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract This inexpensive method for fully automated amino acid analysis combines the advantages of automated precolumn derivatization with o-phthaldialdehyde and favorable analytical conditions to separate and quantify 30 amino acids found in normal plasma. The system can run unattended for almost four days, during which the data are processed automatically by a personal computer and a maximum of 76 samples and 19 standards can be processed (cycle time per analysis: 55 min). Only 1 microL of deproteinized plasma is required per analysis. Coefficients of variation for retention times and areas measured for all relevant amino acids are less than 1% and 3%, respectively. The system described is well suited for quick, sensitive operation in daily practice.

scholarly journals Liquid Chromatographic Determination of Lysine, Methionine, and Threonine in Pure Amino Acids (Feed Grade) and Premixes: Collaborative Study

2000 ◽  
Vol 83 (4) ◽  
pp. 771-783 ◽  
Author(s):  
Johannes Fontaine ◽  
Marcelle Eudaimon ◽  
J Bargholz ◽  
M W Empie ◽  
S Eriksson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Collaborative Study ◽  
Cation Exchange Resin ◽  
Chromatographic Determination ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Standard Deviations ◽  
Liquid Chromatographic

Abstract A total of 17 laboratories (including one author's laboratory) participated in a collaborative study for determination of lysine, methionine, and threonine in trade products or concentrated amino acid premixes. Thirteen samples, 4 pure amino acids and 6 premixes, including 3 Youden matched pairs, were analyzed. The applied liquid chromatographic (LC) method using cation-exchange resin and post-column derivatization with ninhydrin or o-phthaldialdehyde was shown to be accurate and specific for the analytes. Titration procedures, normally used for the assay of pure amino acids, are unspecific and the accuracy of the results can be affected by impurities. Repeatability relative standard deviations, RSDr, ranged from 0.84 to 1.17% for pure amino acids and from 0.50 to 1.68% for premixes; reproducibility relative standard deviations RSDR, ranged from 1.52 to 2.31% for pure amino acids and from 1.48 to 2.59% for premixes. Recoveries were between 97.5 and 102.8% of the expected amino acid assays. The method has been adopted Official First Action status by AOAC INTERNATIONAL.

Sign in / Sign up

Export Citation Format

Share Document