scholarly journals PSII-38 The effect of clitoral stimulation post fixed-time insemination in Bos indicus beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 218-219
Author(s):  
Ramiro Oliveira Filho ◽  
Gustavo Azevedo de Mello ◽  
Victor Mendonça Pereira ◽  
Jose Luiz Moraes Vasconcelos ◽  
Reinaldo F Cooke ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Beneficial Effect ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estrus Synchronization ◽  
Exit Velocity ◽  
Clitoral Stimulation ◽  
To Receive

Abstract The objective of this experiment was to evaluate the effect of clitoral stimulation post-insemination on pregnancy rates in Bos indicus beef cattle. A total of 1186 multiparous Nelore cows were assigned to an estradiol-based estrus synchronization + timed-AI protocol (FTAI, d 0), and randomly assigned to receive either 2 to 5 seconds of clitoral stimulation or no clitoral stimulation after artificial insemination. Estrus expression prior to AI was evaluated based on patch activation (estrus, ≥ 50% activated; no estrus, < 50% activated) using Estrotect Heat Detector and chute exit velocity after AI was evaluated. Individual exit score was calculated within each group by dividing exit velocity into quintiles and assigning cows with a score from 1 to 5 (1 = slowest; 5 = fastest). Artificial insemination was performed by 7 different technicians. Pregnancy diagnosis was performed 30 days after FTAI via transrectal ultrasonography. Overall pregnancy rate at day 30 was 46.0% (545/1186). Clitoral stimulation had no beneficial effect (P = 0.42) on pregnancy rate of multiparous cows when compared to those not stimulated (47.5% ± 4.6 vs. 44.3% ± 4.6). Cows that expressed heat at d 0 (n = 602) had a greater (P < 0.01) pregnancy rate compared to no heat expression (37.6% ± 4.7 vs. 60.6% ± 4.6), but no effect of the clitoral stimulation was observed on pregnancy rate in cows that did or did not express heat (P > 0.05). Furthermore, clitoral stimulation did not affect exit score (P = 0.67) of cows after FTAI. No effect of the technician was observed on pregnancy rate when clitoral stimulation was applied or not (P = 0.21). In summary, clitoral stimulation was not a useful managerial tool to improve pregnancy rate in multiparous Bos indicus cows submitted to FTAI using estradiol-based protocols.

scholarly journals The effect of clitoral stimulation post artificial insemination on pregnancy rates of multiparous Bos indicus beef cows submitted to estradiol/progesterone-based estrus synchronization protocol

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Ramiro V Oliveira Filho ◽  
Reinaldo F Cooke ◽  
Gustavo A de Mello ◽  
Victor M Pereira ◽  
Jose Luiz M Vasconcelos ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Exit Velocity ◽  
Clitoral Stimulation

Abstract Clitoral stimulation after artificial insemination (AI) in beef cattle is a common practice utilized by AI technicians; however, the effect of clitoral stimulation during fixed-time AI (TAI) is still unknown. The objective of the present experiment was to evaluate the effect of clitoral stimulation on temperament responses and pregnancy rates to TAI in Bos indicus beef cows. A total of 1,186 multiparous Nellore cows across three different locations were assigned to an estradiol/progesterone-based estrus synchronization + TAI protocol (day −11 to 0). Cows were randomly assigned to receive either 3 s of clitoral stimulation (n = 602) or no clitoral stimulation (n = 584) immediately after TAI (day 0). Cow body condition score (BCS) was recorded on day −11. Estrus expression was evaluated based on estrus detection visual aid patch activation on day 0 (estrus, ≥50% activated; no estrus, <50% activated). Temperament was assessed by individual chute score based on a 5-point scale before TAI, and individual exit velocity was measured after clitoral stimulation. Pregnancy diagnosis was performed 30 d after TAI via transrectal ultrasonography. Pregnancy rate to TAI was positively affected by BCS (P < 0.01) and estrus expression (P = 0.03). Pregnancy rates of cows receiving clitoral stimulation did not differ (P = 0.39) from cows non-stimulated (47.5 ± 4.6% vs. 44.3 ± 4.6%, respectively). No interaction of clitoral stimulation and estrus expression was observed (P = 0.26). Chute score was positively correlated to exit velocity (P < 0.01; r = 0.29); however, clitoral stimulation did not affect exit velocity (P = 0.86). In summary, pregnancy rates to TAI are influenced by various factors and multiple strategies have the potential to increase the fertility of beef cows submitted to TAI; however, clitoral stimulation of Bos indicus beef cows did not improve TAI pregnancy rate.

510 Impact of estrus synchronization and fixed-time artificial insemination on calving distribution in Bos indicus influenced beef heifers

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 249-249
Author(s):  
N. Oosthuizen ◽  
P. L. P. Fontes ◽  
C. D. Sanford ◽  
F. M. Ciriaco ◽  
D. D. Henry ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estrus Synchronization ◽  
Beef Heifers

scholarly journals Pregnancy rate in lactating Bos indicus cows subjected to fixed-time artificial insemination and treated with different follicular growth inducers

2014 ◽  
Vol 43 (7) ◽  
pp. 358-362 ◽  
Author(s):  
Ériklis Nogueira ◽  
Dayanna Schiavi do Nascimento Batista ◽  
Luiz Carlos Cesar da Costa Filho ◽  
Alexandre Menezes Dias ◽  
Juliana Corrêa Borges Silva ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Bos Indicus ◽  
Fixed Time ◽  
Follicular Growth

287 Effects of Number of Sperm and Site of Uterine Semen Deposition on Conception Rate and Number of Embryos in Weaned Sows Receiving a Single Fixed Time Insemination

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 111-111
Author(s):  
Kara R Stewart ◽  
Brad Belstra ◽  
Kilby L Willenburg ◽  
Domingo Gómez-López ◽  
Robert V Knox
Keyword(s):  
Pregnancy Rate ◽  
Litter Size ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Induction Method ◽  
Rate Range ◽  
Litter Traits ◽  
Pregnancy Status ◽  
Follicle Size ◽  
To Receive

Abstract Induced ovulation with single fixed time artificial insemination (SFTAI), combined with uterine (IUI) or deep uterine insemination (DUI), could improve fertility with low numbers of sperm and allow greater use of high genetic merit boars. At weaning (0 h), sows (n = 534) were assigned by parity and estrus induction method (eCG or Control) to receive 1200 × 106 sperm by IUI, 600, 300, or 150 × 106 sperm by IUI or DUI, or 75 × 106 sperm by DUI. At 80 h post weaning, sows received OvuGel and 26 h later a pooled semen SFTAI. Ultrasound was performed to determine follicle size and time of ovulation (OV). Sows were slaughtered 27 d after AI to determine pregnancy and litter traits. There was no effect (P > 0.05) of eCG on estrus (93%) within 5 d of weaning or follicle size (6.1 mm) at OvuGel, but wean-to-estrus (3.8 vs. 4.0 d,P < 0.01) and AI-to-OV (15.9 vs. 17.0 h, P = 0.04) intervals and AI-to OV were slightly reduced. eCG did not affect pregnancy rate (78.6%), number of CL (21.7), or number of viable embryos (12.2). There was no effect of number of sperm or site of insemination (P > 0.05) on pregnancy rate (range: 80.9% to 70.5%), but AI occurring after ovulation reduced pregnancy rate (P < 0.02). Total number of embryos (range: 16.5 to 10.3) increased with CL number (P < 0.001) but was not affected by number of sperm or site of insemination (P > 0.05). Higher sperm treatments (1200 and 600 x 106) had more embryos compared to lower sperm treatments (P < 0.01), suggesting that lower sperm numbers effects litter size more than the pregnancy status. Acceptable fertility can be achieved with low sperm numbers when using SFTAI and uterine deposition, but AI-to-OV interval and ovulation rate influence final fertility.

scholarly journals Inbreeding Depression and Alternative Solution in Buffaloes

2018 ◽  
Vol 28 (1) ◽  
pp. 1
Author(s):  
Lisa Praharani ◽  
Riasari Gail Sianturi ◽  
Chalid Talib
Keyword(s):  
Growth Performance ◽  
Pregnancy Rate ◽  
Inbreeding Depression ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Farming System ◽  
Alternative Solution ◽  
Selling Price ◽  
Estrus Synchronization ◽  
Natural Breeding

Buff and dairy buffaloes have an important role in farming system to produce meat, milk, and fertilizer. Their productivity and population have been decreasing due to several reasons such as inbreeding depression and lack of sires. This paper presents alternative efforts to improve genetics of buffaloes through outbreeding fascilitated by artificial insemination (AI) and estrus synchronization to decrease inbreeding rates. Effort to reduce inbreeding depression is conducted by introducing new buffalo genes from distant populations known as outbreeding. Indonesian Research Institute for Animal Production has initiated an outbreeding program in buffalo through AI. The pregnancy rate varies between 40-80% using estrus synchronization with prostaglandin and fixed time AI. Outbred offsprings have higher growth performance and selling price than offsprings of natural breeding. The outbreeding program through AI in buffaloes need to be carried out sustainability to increase availability of qualified breeding stocks.

Estrus synchronization and fixed-time artificial insemination alter calving distribution in Bos indicus influenced beef heifers

2018 ◽  
Vol 106 ◽  
pp. 210-213 ◽  
Author(s):  
N. Oosthuizen ◽  
P.L.P. Fontes ◽  
C.D. Sanford ◽  
F.M. Ciriaco ◽  
D.D. Henry ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estrus Synchronization ◽  
Beef Heifers

Comparison of progestin-based estrus synchronization protocols before fixed-time artificial insemination on pregnancy rate in beef heifers1

2007 ◽  
Vol 85 (8) ◽  
pp. 1933-1939 ◽  
Author(s):  
D. C. Busch ◽  
D. J. Wilson ◽  
D. J. Schafer ◽  
N. R. Leitman ◽  
J. K. Haden ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Estrus Synchronization

scholarly journals 218 Effect of GnRH injection at -72 h in MGA-PG estrus synchronization protocol

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 122-122
Author(s):  
McKay Erickson ◽  
Rick N Funston
Keyword(s):  
Pregnancy Rate ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Estrus Detection ◽  
To Receive ◽  
Observation Period ◽  
Synchronization Protocol

Abstract Beef heifers (n = 1524) from two operations in central Nebraska were randomly assigned to one of two treatments [0 or 5 μg GnRH at prostaglandin F2α (PGF) administration 72 hrs before insemination]. Both locations utilized MGA-PG fixed-time AI (TAI) estrus synchronization protocol. The first location (L1, n = 1076, 382 ± 3 kg) processed heifers 72 hours prior to insemination and every third heifer was assigned to receive an injection of GnRH (5 ug) (TRT) and an injection of PGF. The remaining heifers received PGF and 0 μg GnRH. At insemination all heifers received 100 ug GnRH. Heifers were then observed for estrus behavior from 10–21 days post TAI and re-inseminated if estrus was detected. Heifers pregnant from the second breeding were added to final pregnancy rate. The second location (L2) utilized estrus detection patches and performed only TAI (n = 448, 363 ± 7 kg) following MGA-PG synchronization protocol outlined above without the estrus observation period. Treatment did not significantly (P > 0.20) improve TAI pregnancy rates [L1 TAI 56.3% (TRT) vs 57.3%; L1 AI Total 78.3% (TRT) vs 73.9%; L2 TAI 58.5% (TRT) vs 52.5%] among the two herds. The administration of 5 μg GnRH at PGF tended to increase (P = 0.12, 74% vs 63%) pregnancy rates for those inseminated during the follow up heat detection period at L1. The treatment did not (P > 0.20) improve pregnancy for the time-AI heifers. In addition, TAI pregnancy rates were similar (P > 0.20) at L2. There were greater (P < 0.20) effect of pen on pregnancy rate.

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