Nickel-Binding Properties of the C-Terminal Tail Peptide of Bacillus pasteurii UreE

2004 ◽  
Vol 136 (5) ◽  
pp. 635-641 ◽  
Author(s):  
H.-S. Won
Keyword(s):  
Bacillus Pasteurii ◽  
Binding Properties ◽  
Nickel Binding

scholarly journals A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains

2021 ◽  
Vol 22 (24) ◽  
pp. 13210
Author(s):  
Danuta Witkowska ◽  
Agnieszka Szebesczyk ◽  
Joanna Wątły ◽  
Michał Braczkowski ◽  
Magdalena Rowińska-Żyrek
Keyword(s):  
Helicobacter Pylori ◽  
Comparative Study ◽  
Potentiometric Titration ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Binding Properties ◽  
Protein Fragments ◽  
Nickel Binding ◽  
Independent Reaction

Combined potentiometric titration and isothermal titration calorimetry (ITC) methods were used to study the interactions of nickel(II) ions with the N-terminal fragments and histidine-rich fragments of Hpn-like protein from two Helicobacter pylori strains (11637 and 26695). The ITC measurements were performed at various temperatures and buffers in order to extract proton-independent reaction enthalpies of nickel binding to each of the studied protein fragments. We bring up the problem of ITC results of nickel binding to the Hpn-like protein being not always compatible with those from potentiometry and MS regarding the stoichiometry and affinity. The roles of the ATCUN motif and multiple His and Gln residues in Ni(II) binding are discussed. The results provided the possibility to compare the Ni(II) binding properties between N-terminal and histidine-rich part of Hpn-like protein and between N-terminal parts of two Hpn-like strains, which differ mainly in the number of glutamine residues.

scholarly journals Structural Characterization of the Nickel-binding Properties ofBacillus pasteuriiUrease Accessory Protein (Ure)E in Solution

2004 ◽  
Vol 279 (17) ◽  
pp. 17466-17472 ◽  
Author(s):  
Hyung-Sik Won ◽  
Yeon-Hee Lee ◽  
Ji-Hun Kim ◽  
In Seon Shin ◽  
Mann Hyung Lee ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Accessory Protein ◽  
Binding Properties ◽  
Nickel Binding

scholarly journals Nickel binding properties of Helicobacter pylori UreF, an accessory protein in the nickel-based activation of urease

2013 ◽  
Vol 19 (3) ◽  
pp. 319-334 ◽  
Author(s):  
Barbara Zambelli ◽  
Andrea Berardi ◽  
Vlad Martin-Diaconescu ◽  
Luca Mazzei ◽  
Francesco Musiani ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Accessory Protein ◽  
Binding Properties ◽  
Nickel Binding

scholarly journals Biophysical Examination of the Calcium-Modulated Nickel-Binding Properties of Human Calprotectin Reveals Conformational Change in the EF-Hand Domains and His3Asp Site

Biochemistry ◽  
2018 ◽  
Vol 57 (28) ◽  
pp. 4155-4164 ◽  
Author(s):  
Toshiki G. Nakashige ◽  
Sarah E. J. Bowman ◽  
Emily M. Zygiel ◽  
Catherine L. Drennan ◽  
Elizabeth M. Nolan
Keyword(s):  
Conformational Change ◽  
Binding Properties ◽  
Nickel Binding ◽  
Ef Hand

A new family of fluorescent calcium indicators designed to resist leakage or for measuring calcium near membranes

1994 ◽  
Vol 52 ◽  
pp. 168-169
Author(s):  
Martin Poenie ◽  
Akwasi Minta ◽  
Charles Vorndran
Keyword(s):  
Metal Binding ◽  
Acetoxymethyl Ester ◽  
Binding Properties ◽  
Fura 2 ◽  
New Family ◽  
Anion Transporter ◽  
Calcium Indicator ◽  
Calcium Indicators ◽  
High Calcium ◽  
Intracellular Compartmentalization

The use of fura-2 as an intracellular calcium indicator is complicated by problems of rapid dye leakage and intracellular compartmentalization which is due to a probenecid sensitive anion transporter. In addition there is increasing evidence for localized microdomains of high calcium signals which may not be faithfully reported by fura-2.We have developed a new family of fura-2 analogs aimed at addressing some of these problems. These new indicators are based on a modified bapta which can be readily derivatized to produce fura-2 analogs with a variety of new properties. The modifications do not affect the chromophore and have little impact on the spectral and metal binding properties of the indicator. One of these new derivatives known as FPE3 is a zwitterionic analog of fura-2 that can be loaded into cells as an acetoxymethyl ester and whose retention in cells is much improved. The improved retention of FPE3 is important for both cuvettebased measurements of cell suspensions and for calcium imaging.

Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin

1998 ◽  
Vol 36 (5) ◽  
pp. 291-298 ◽  
Author(s):  
DE LUCCA ◽  
BLAND ◽  
JACKS ◽  
GRIMM ◽  
WALSH
Keyword(s):  
Binding Properties ◽  
Cecropin B ◽  
Natural Peptides
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