Characteristics of “Hybrid”-Type Clonal Cell Lines Obtained From Mixed Cultures In Vitro2

1962 ◽  
Keyword(s):  
Cell Lines ◽  
Mixed Cultures ◽  
Clonal Cell ◽  
Hybrid Type ◽  
Clonal Cell Lines

scholarly journals Plasticity of Hepatic Cell Differentiation: Bipotential Adult Mouse Liver Clonal Cell Lines Competent to Differentiate In Vitro and In Vivo

Stem Cells ◽  
2006 ◽  
Vol 24 (9) ◽  
pp. 2098-2109 ◽  
Author(s):  
Catherine Fougère-Deschatrette ◽  
Tereza Imaizumi-Scherrer ◽  
Hélène Strick-Marchand ◽  
Serban Morosan ◽  
Pierre Charneau ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Cell Lines ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Hepatic Cell ◽  
Clonal Cell ◽  
Clonal Cell Lines ◽  
Adult Mouse Liver

scholarly journals Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.

1988 ◽  
Vol 8 (4) ◽  
pp. 1664-1669 ◽  
Author(s):  
K M Karrer ◽  
M C Yao
Keyword(s):  
Cell Lines ◽  
Transformation Efficiency ◽  
Tetrahymena Thermophila ◽  
Host Cells ◽  
Rrna Genes ◽  
Major Constituent ◽  
Clonal Cell ◽  
Clonal Cell Lines

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations.

276 PRODUCTION OF TRANSGENIC MINI-PIG CELL LINES EXPRESSING HUMAN CYTOMEGALOVIRUS US2

2007 ◽  
Vol 19 (1) ◽  
pp. 254
Author(s):  
K. W. Park ◽  
J. Y. Yoo ◽  
K. M. Choi ◽  
S. P. Hong ◽  
G. S. Han ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Recombinant Expression ◽  
Class I ◽  
Class I Mhc ◽  
Specific Lysis ◽  
Clonal Cell ◽  
Fetal Fibroblasts ◽  
Clonal Cell Lines

Xenotransplantation has the potential to resolve the chronic shortage of donor organs if immunological barriers can be overcome. In particular, the initial type of rejection following xenotransplantation is acute cellular rejection by host CD8+ cytotoxic T lymphocyte (CTL) cells that react to the donor class I major histocompatibility complex (MHC). The human cytomegalovirus (HCMV) glycoprotein US2 specifically targets class I MHC heavy chains for dislocation from the endoplasmic reticulum (ER) membrane to the cytosol, where they are degraded by the proteasome. In this study, the recombinant expression vector pCX-US2 was stably transfected into mini-pig fetal fibroblasts by lipofection. The integration of US2 into the host genome was confirmed by PCR and Southern blot assay. The reduction of swine leukocyte antigen class I (SLA-I, MHC protein class I) by US2 was detected by flow cytometry analysis (FACS). FACS analysis of US2 clonal cell lines demonstrated substantial reductions in SLA surface expression. The decrease in the level of class I MHC expression for US2 clonal cell lines ranged from 22 to 34% relative to the non-transfected control. US2 clonal cell lines were also tested to determine if the resulting reduction in cell surface SLA would reduce in vitro cytotoxicity by CTL. The US2 clonal cell line demonstrated 5- to 6-fold reduction of specific lysis by primed CD8+ CTL. In conclusion, US2 can directly protect pig clonal cell lines from human CTL cells. These results indicate that the expression of US2 in pig cells may provide a new approach toward overcoming CTL-mediated immunity to xenotransplantation. This work was supported by the National Livestock Research Institute (6132-211-303-1).

Interleukin-6 stimulates cell proliferation of rat pituitary clonal cell lines in vitro

1995 ◽  
Vol 18 (2) ◽  
pp. 83-90 ◽  
Author(s):  
T. Sawada ◽  
Koji Koike ◽  
Y. Kanda ◽  
H. Ikegami ◽  
H. Jikihara ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Interleukin 6 ◽  
Clonal Cell ◽  
Rat Pituitary ◽  
Clonal Cell Lines

scholarly journals Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro

2008 ◽  
Vol 237 (5) ◽  
pp. 1412-1423 ◽  
Author(s):  
J. Peltzer ◽  
L. Colman ◽  
J. Cebrian ◽  
H. Musa ◽  
M. Peckham ◽  
...  
Keyword(s):  
Cell Lines ◽  
Slow Muscle ◽  
Clonal Cell ◽  
Fast And Slow Muscle ◽  
Clonal Cell Lines

scholarly journals Transformation of Tetrahymena thermophila with hypermethylated rRNA genes

1988 ◽  
Vol 8 (4) ◽  
pp. 1664-1669
Author(s):  
K M Karrer ◽  
M C Yao
Keyword(s):  
Cell Lines ◽  
Transformation Efficiency ◽  
Tetrahymena Thermophila ◽  
Host Cells ◽  
Rrna Genes ◽  
Major Constituent ◽  
Clonal Cell ◽  
Clonal Cell Lines

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations.

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