Enzyme Activities and Extrinsic Proteins in Plasma Membranes From Normal Liver and Morris Hepatoma 5123tc 2

1973 ◽  
Vol 51 (5) ◽  
pp. 1709-1710 ◽  
Author(s):  
M. Barclay ◽  
O. Terebus-Kekish
Keyword(s):  
Enzyme Activities ◽  
Plasma Membranes ◽  
Normal Liver ◽  
Morris Hepatoma ◽  
Extrinsic Proteins

Feed-borne exposure to deoxynivalenol leads to acute and chronic effects on liver enzymes and histology in carp

2015 ◽  
Vol 8 (5) ◽  
pp. 619-627 ◽  
Author(s):  
C. Pietsch ◽  
P. Burkhardt-Holm
Keyword(s):  
Liver Enzyme ◽  
Enzyme Activities ◽  
Liver Tissue ◽  
Time Course ◽  
Normal Liver ◽  
Fish Feed ◽  
Acute Response ◽  
Biotransformation Enzymes ◽  
Chronic Effects ◽  
Acute And Chronic Effects

Deoxynivalenol (DON) is a frequent contaminant of feeds in aquaculture, but the consequences of this contamination have rarely been evaluated. Previous studies on carp indicated effects of DON on liver function and histology after four weeks of feeding. The present study aimed to unravel the time course of liver responses of carp to orally applied DON. Therefore, liver enzyme activities and histology have been investigated after 7, 14, 26 and 56 days of DON feeding. The acute response comprises down-regulation of biotransformation enzymes, whereas the chronic response to DON is characterised by activation of alanine aminotransferase which indicates damage to liver tissue. Examination of histological sections of liver tissue revealed that changes such as fat aggregation, vacuolisation and hyperaemia were present after 14 and 26 days of exposure to DON but not thereafter. Several enzymes involved in glutathione cycling and reduction of oxidative stress were found to be reduced after 26 and 56 days of DON feeding. The results suggest that supporting the antioxidative system, e.g. by using glutathione-enriched yeast extracts as a food additive, might be successful in preventing the effects of DON in carp. This is the basis of a fundamental hypothesis since DON contamination of fish feed leads to pronounced effects on liver histology and liver enzyme activities which may also cause changes in the normal liver metabolism of endogenous and xenobiotic compounds.

Ionizing radiation stimulates muscarinic regulation of rat intestinal mucosal function

1998 ◽  
Vol 275 (6) ◽  
pp. G1333-G1340 ◽  
Author(s):  
F. Lebrun ◽  
A. Francois ◽  
M. Vergnet ◽  
L. Lebaron-Jacobs ◽  
P. Gourmelon ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Enzyme Activities ◽  
Plasma Membranes ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Marker Enzyme ◽  
Γ Irradiation ◽  
Ussing Chambers ◽  
Radiation Induced ◽  
Total Body

The aim of this study was to determine whether ionizing radiation modifies muscarinic regulation of intestinal mucosal function. Rats exposed to total body 8-Gy γ-irradiation or sham irradiated were studied up to 21 days after irradiation. Basal and carbachol-stimulated short-circuit current ( I sc) and transepithelial conductance ( G t) of stripped ileum were determined in Ussing chambers. Muscarinic receptor characteristics using the muscarinic antagonist [3H]quinuclidinyl benzilate and three unlabeled antagonists were measured in small intestinal plasma membranes together with two marker enzyme activities (sucrase, Na+-K+-ATPase). Enzyme activities were decreased 4 days after irradiation ( day 4). Basal electrical parameters were unchanged. Maximal carbachol-induced changes in I sc and G t were increased at day 4 (maximal Δ I sc = 195.8 ± 14.7 μA/cm2, n = 19, vs. 115.4 ± 8.2 μA/cm2, n = 63, for control rats) and unchanged at day 7. Dissociation constant was decreased at day 4 (0.73 ± 0.29 nM, n = 10, vs. 2.14 ± 0.39 nM, n = 13, for control rats) but unchanged at day 7, without change in binding site number. Thus total body irradiation induces a temporary stimulation of cholinergic regulation of mucosal intestinal function that may result in radiation-induced diarrhea.

scholarly journals Interaction of fluorescent probes with plasma membranes from rat liver and morris hepatoma 3924A

FEBS Letters ◽  
1975 ◽  
Vol 49 (3) ◽  
pp. 346-349 ◽  
Author(s):  
Ornella Dionisi ◽  
Tommaso Galeotti ◽  
Tullio Terranova ◽  
Paola Arslan ◽  
Angelo Azzi
Keyword(s):  
Rat Liver ◽  
Fluorescent Probes ◽  
Plasma Membranes ◽  
Morris Hepatoma

Glycogen metabolism in normal liver and Morris Hepatoma 7787 in meal-fed rat

1980 ◽  
Vol 12 (3) ◽  
pp. 371-378 ◽  
Author(s):  
P.John Anderson ◽  
Susan A. Rotenberg ◽  
Harold P. Morris ◽  
Barbara E. Wright
Keyword(s):  
Normal Liver ◽  
Glycogen Metabolism ◽  
Morris Hepatoma

scholarly journals A discoordinate increase in the cellular amount of 3-hydroxy-3-methylglutaryl-CoA reductase results in the loss of rate-limiting control over cholesterogenesis in a tumour cell-free system

1989 ◽  
Vol 258 (2) ◽  
pp. 421-425 ◽  
Author(s):  
N I Azrolan ◽  
P S Coleman
Keyword(s):  
Cholesterol Biosynthesis ◽  
Normal Liver ◽  
Preliminary Evidence ◽  
Free System ◽  
Normal Rat ◽  
Morris Hepatoma ◽  
Coa Reductase ◽  
Rate Limiting ◽  
Hydroxymethylglutaryl Coa Reductase ◽  
Tumour System

Cholesterol biosynthesis was characterized in cell-free post-mitochondrial supernatant systems prepared from both normal rat liver and Morris hepatoma 3924A. The rate of cholesterol synthesis per cell was 9-fold greater in the tumour system than in that from normal liver, and the tumour systems showed the loss of rate-limiting control at the hydroxymethylglutaryl-CoA reductase (HMGR)-catalysed step. The apparent absence of rate-limiting control over cell-free tumour cholesterogenesis was traced primarily to a discoordinate and dramatic increase in the amount of HMGR in the tumour relative to the liver system. Preliminary evidence for an altered control of the post-lanosterol portion of the pathway was also obtained with the tumour system.

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