Advantages Cryopreserved Lymphocytes for Sequential Evaluation of Human Immune Competence. II. Mixed Lymphocyte Cultures and of Mononuclear Cell Subpopulations

1978 ◽  
Keyword(s):  
Mononuclear Cell ◽  
Immune Competence ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures ◽  
Human Immune ◽  
Cell Subpopulations ◽  
Sequential Evaluation

scholarly journals IMMUNOLOGIC PROPERTIES OF MOUSE THYMUS CELLS

1972 ◽  
Vol 136 (6) ◽  
pp. 1461-1477 ◽  
Author(s):  
Susumu Konda ◽  
Yoshinobu Nakao ◽  
Richard T. Smith
Keyword(s):  
Gradient Centrifugation ◽  
F1 Hybrid ◽  
Thymus Cell ◽  
Mouse Thymus ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures ◽  
High Concentrations ◽  
Cell Subpopulations ◽  
Thymus Cells

The functional attributes of minor subpopulations of mouse thymus cells derived by bovine serum albumin density gradient centrifugation, cortisone treatment, or selective depletion by anti-TL or anti-θ treatment have been examined. A subpopulation derived in each fashion contains the cells required to evoke graft-versus-host reactions in neonatal F1 hybrid recipients and to be stimulated by alloantigens in vitro in one-way mixed lymphocyte cultures and by phytohemagglutinin. The functions of this subpopulation are abrogated by treatment with anti-H-2 plus complement and by high concentrations of anti-θ. A tentative ordering of the various thymus cell subpopulations, on the basis of these and other data, is described.

scholarly journals QUANTITATIVE STUDIES ON THE MIXED LYMPHOCYTE INTERACTION IN RATS

1971 ◽  
Vol 134 (4) ◽  
pp. 857-870 ◽  
Author(s):  
Darcy B. Wilson ◽  
Dianne H. Fox
Keyword(s):  
Cell Surface ◽  
Surface Antigens ◽  
Mouse Cell ◽  
Cell Surface Antigens ◽  
Quantitative Studies ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures ◽  
Environmental Antigens ◽  
Germfree Rats ◽  
Number Of Cells

The proliferative reactivity of lymphocytes from rat donors maintained under germfree or conventional conditions was examined in mixed lymphocyte cultures stimulated with allogeneic and xenogeneic cell surface antigens. The results show (a) that lymphocytes from conventionally maintained rats are less reactive to human, hamster, guinea pig, and mouse cell surface antigens than to the major H alloantigens, and (b) that lymphocytes from germfree rats display no demonstrable reactivity to xenogeneic cells, but are quantitatively normal in their response to allogenic cells. The conclusion drawn from these observations is that the circulating lymphocyte pool of an individual consists of a greater proportion of cells reactive to H alloantigens of other members of the same species than to the xenogeneic cellular antigens of members of other species and that this large number of cells is not generated by a mechanism involving immunization to cross-reactive environmental antigens.

Effect of Heparin on Mixed Lymphocyte Cultures

Nature ◽  
1967 ◽  
Vol 215 (5097) ◽  
pp. 164-165 ◽  
Author(s):  
G. A. CURRIE
Keyword(s):  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures

Mixed Lymphocyte Cultures with Inbred Individuals: An Approach to MLC Typing

2008 ◽  
Vol 3 (5) ◽  
pp. 323-339 ◽  
Author(s):  
F. Jørgensen ◽  
L. U. Lamm ◽  
F. Kissmeyer-nielsen
Keyword(s):  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures
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