Characterization of Tumorigenic and Metastatic Properties of Murine Sarcoma Virus-Transformed Nonproducer Balb/3T3 Cell Lines23

1980 ◽  
Keyword(s):  
Cell Lines ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
3T3 Cell Lines ◽  
Murine Sarcoma

Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells

1982 ◽  
Vol 2 (1) ◽  
pp. 42-51
Author(s):  
S Gattoni ◽  
P Kirschmeier ◽  
I B Weinstein ◽  
J Escobedo ◽  
D Dina
Keyword(s):  
Cell Lines ◽  
Blot Hybridization ◽  
Cell Types ◽  
Single Copy ◽  
Transformed Cell Line ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Blotting Technique ◽  
Moloney Sarcoma Virus ◽  
Murine Sarcoma

Moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. Because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agents, we have examined the state of integration, methylation, and transcriptional activity of c-mos sequences in a variety of normal rodent tissues, normal cell lines, or cell lines transformed by radiation or chemical carcinogens. DNA-DNA hybridization, utilizing the Southern blotting technique and a plasmid-derived DNA probe representing the v-mos sequence, gave no evidence for rearrangements of the c-mos sequence in the DNAs obtained from these diverse cell types. Parallel studies employing the restriction enzyme isoschizomers HpaII and MspI indicated that in all of these cell types the c-mos sequences were heavily methylated. In addition, analysis of cellular RNAs by blot hybridization with the v-mos probe failed to detect evidence of transcription of the c-mos sequences in any of these cell types. This was in contrast to a Moloney sarcoma virus-transformed cell line in which we found that the integrated v-mos sequence was both undermethylated and extensively transcribed. Thus, it would appear that c-mos sequences do not play a role in the transformation of rodent cells by chemical or physical agents, although the possible role of other endogenous onc sequences remains to be determined.

scholarly journals Expression of the onc Gene of the Kirsten Murine Sarcoma Virus in Differentiated Rat Thyroid Epithelial Cell Lines

1984 ◽  
Vol 65 (11) ◽  
pp. 1955-1961 ◽  
Author(s):  
M. Ferrentino ◽  
P. P. Di Fiore ◽  
A. Fusco ◽  
G. Colletta ◽  
A. Pinto ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Thyroid Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rat Thyroid ◽  
Murine Sarcoma

Studies on murine sarcoma virus: antigenic characterization of murine sarcoma virus induced tumor cells

1969 ◽  
Vol 4 (4) ◽  
pp. 465-479 ◽  
Author(s):  
Jean-Claude Chuat ◽  
Leonard Berman ◽  
Peter Gunvén ◽  
Eva Klein
Keyword(s):  
Tumor Cells ◽  
Antigenic Characterization ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma
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