scholarly journals Expression of the onc Gene of the Kirsten Murine Sarcoma Virus in Differentiated Rat Thyroid Epithelial Cell Lines

1984 ◽  
Vol 65 (11) ◽  
pp. 1955-1961 ◽  
Author(s):  
M. Ferrentino ◽  
P. P. Di Fiore ◽  
A. Fusco ◽  
G. Colletta ◽  
A. Pinto ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Thyroid Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rat Thyroid ◽  
Murine Sarcoma

Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells

1982 ◽  
Vol 2 (1) ◽  
pp. 42-51
Author(s):  
S Gattoni ◽  
P Kirschmeier ◽  
I B Weinstein ◽  
J Escobedo ◽  
D Dina
Keyword(s):  
Cell Lines ◽  
Blot Hybridization ◽  
Cell Types ◽  
Single Copy ◽  
Transformed Cell Line ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Blotting Technique ◽  
Moloney Sarcoma Virus ◽  
Murine Sarcoma

Moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. Because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agents, we have examined the state of integration, methylation, and transcriptional activity of c-mos sequences in a variety of normal rodent tissues, normal cell lines, or cell lines transformed by radiation or chemical carcinogens. DNA-DNA hybridization, utilizing the Southern blotting technique and a plasmid-derived DNA probe representing the v-mos sequence, gave no evidence for rearrangements of the c-mos sequence in the DNAs obtained from these diverse cell types. Parallel studies employing the restriction enzyme isoschizomers HpaII and MspI indicated that in all of these cell types the c-mos sequences were heavily methylated. In addition, analysis of cellular RNAs by blot hybridization with the v-mos probe failed to detect evidence of transcription of the c-mos sequences in any of these cell types. This was in contrast to a Moloney sarcoma virus-transformed cell line in which we found that the integrated v-mos sequence was both undermethylated and extensively transcribed. Thus, it would appear that c-mos sequences do not play a role in the transformation of rodent cells by chemical or physical agents, although the possible role of other endogenous onc sequences remains to be determined.

scholarly journals Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus.

1983 ◽  
Vol 3 (11) ◽  
pp. 2099-2109 ◽  
Author(s):  
G Colletta ◽  
A Pinto ◽  
P P Di Fiore ◽  
A Fusco ◽  
M Ferrentino ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Functional Markers ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Phenotypic Properties ◽  
Temperature Sensitive Mutant ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rat Thyroid ◽  
Murine Sarcoma

Differentiated rat thyroid epithelial cells, infected in vitro with a temperature-sensitive mutant of the Kirsten murine sarcoma virus, expressed at the permissive temperature (33 degrees C) some phenotypic properties typical of transformed cells, including morphological features, colony formation in agar, and induction of tumors in newborn animals. Specific functional markers of these differentiated cells, i.e., synthesis/secretion of thyroglobulin, synthesis of thyroglobulin mRNA and iodide uptake, were blocked during growth at 33 degrees C. Normal morphology, failure to grow in agar, and the requirement of hormones for optimal growth were all restored after shifting to the temperature nonpermissive for transformation (39 degrees C), though the typical differentiated functions remained blocked. Infection with a leukemia helper virus clone (Moloney or Kirsten murine leukemia virus) did not lead to the loss of the differentiated phenotype of rat epithelial thyroid cells, thus demonstrating that the loss of the differentiated phenotype is caused by the sarcoma virus component. These results indicate that the expression of some of the phenotypic properties of transformed differentiated rat thyroid epithelial cells is under the direct control of the p21 thermosensitive activity, whereas the block in the expression of two typical differentiation markers of thyroid epithelial cells is irreversible and probably controlled by different mechanisms.

scholarly journals Expression of the c-raf protooncogene in human hematopoietic cells and cell lines

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1437-1440 ◽  
Author(s):  
E Sariban ◽  
T Mitchell ◽  
D Kufe
Keyword(s):  
Cell Lines ◽  
Hematopoietic Cells ◽  
Leukemic Cells ◽  
Detectable Effect ◽  
Mouse Macrophages ◽  
Murine Sarcoma Virus ◽  
Monocytes And Macrophages ◽  
Sarcoma Virus ◽  
Murine Sarcoma

Abstract The murine sarcoma virus 3611 contains the transforming v-raf gene that has partial nucleotide homology with the src family of tyrosine kinase- encoding oncogenes. Although this virus induces fibrosarcomas in mice, a recombinant murine retrovirus carrying both the raf and myc oncogenes induces immunoblastic lymphomas and immortalizes mouse macrophages in vitro. The present study has thus monitored the expression of c-raf in human hematopoietic cells. The results demonstrate the presence of a 3.6-kb c-raf transcript in HL-60 promyelocytic leukemic cells. The induction of HL-60 cell differentiation along the monocytic or granulocytic lineages had no detectable effect on the level of c-raf transcripts. Furthermore, in contrast to c-myc and c-fms expression, inhibition of protein synthesis with cycloheximide had no detectable effect on c-raf expression. Similar levels of c-rafRNA were also found in other human cell lines derived from myeloid, B cell, and T cell tumors, as well as in normal granulocytes, monocytes, and macrophages. These findings suggest that the c-raf protooncogene is widely expressed in multiple hematopoietic lineages.

scholarly journals MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUSES

1973 ◽  
Vol 138 (2) ◽  
pp. 356-363 ◽  
Author(s):  
M. Hatanaka ◽  
R. Klein ◽  
R. Toni ◽  
J. Walker ◽  
R. Gilden
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Virus Particles ◽  
Tumorigenic Potential ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Type C ◽  
Murine Sarcoma ◽  
Tumorigenic Cell

A variety of cell mutants were obtained by a single 5'-bromodeoxyuridine (BrdU) treatment of an nonproducer (NP) cell line transformed by the Kirsten strain of murine sarcoma virus (Ki-MSV). Isolation procedures of these cell See PDF for Structure mutants are described. The cell mutants obtained were classified by tumorigenic potential and shedding of Type C virus particles. The cell mutants were classified into four groups: (A) tumorigenic, without particles; (B) tumorigenic, with Type C particles; (C) nontumorigenic, without particles; and (D) nontumorigenic, with Type C particles. The tumorigenic cell lines showed variability in morphology with both flat and typical transformed appearing cell lines showing equal transplantability.

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