Suppression of Tumorigenicity in Somatic Cell Hybrids. II. Human Chromosomes Implicated as Suppressors of Tumorigenicity in Hybrids With Chinese Hamster Ovary Cells23

1983 ◽  
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Ovary Cells ◽  
Cell Hybrids

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

1973 ◽  
Vol 20 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Ans Jongsma ◽  
Harry Someren ◽  
Andries Westerveld ◽  
Ann Hagemeijer ◽  
Peter Pearson
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

scholarly journals Genetic effects of chromosomal rearrangements in Chinese hamster ovary cells: expression and chromosomal assignment of TK, GALK, ACP1, ADA, and ITPA loci.

1983 ◽  
Vol 3 (11) ◽  
pp. 1967-1974 ◽  
Author(s):  
R L Stallings ◽  
G M Adair ◽  
J Siciliano ◽  
J Greenspan ◽  
M J Siciliano
Keyword(s):  
Polyethylene Glycol ◽  
Chinese Hamster Ovary ◽  
Gene Deletion ◽  
Chinese Hamster Ovary Cells ◽  
Chromosomal Rearrangements ◽  
Chinese Hamster ◽  
Chromosome 7 ◽  
Chromosomal Assignment ◽  
Somatic Cell Hybrids ◽  
Ovary Cells

Polyethylene glycol-mediated fusion of Chinese hamster ovary (CHO) cells with mouse Cl1D cells produced interspecific somatic cell hybrids which slowly segregated CHO chromosomes. Cytogenetic and isozyme analysis of HAT- and bromodeoxyuridine-selected hybrid subclones and of members of a hybrid clone panel retaining different combinations of CHO chromosomes enabled provisional assignments of the following enzyme loci to CHO chromosomes: TK, GALK, and ACP1 to chromosome 7; TK and GALK to chromosome Z13; ACP1, ADA, and ITPA to chromosome Z8; and ADA and ITPA to chromosome Z9. These genetic markers reflect the origin of each of these Z group chromosomes and indicate the functional activity of alleles located on rearranged chromosomes. Identification of diploid electrophoretic shift mutations for ADA and ITPA was consistent with those observations. Assignment of the functional TK locus in TK+/- CHO-AT3-2 cells indicated that gene deletion may be responsible for TK hemizygosity in this subline.

scholarly journals Multidrug-resistant phenotype cosegregates with an amplified gene in somatic cell hybrids of drug-resistant Chinese hamster ovary cells and drug-sensitive murine cells.

1986 ◽  
Vol 6 (12) ◽  
pp. 4268-4273 ◽  
Author(s):  
L D Teeter ◽  
J A Sanford ◽  
S Sen ◽  
R L Stallings ◽  
M J Siciliano ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Somatic Cell ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Cross Resistance ◽  
Drug Resistant ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Primary Drug Resistance

Gene amplification has been associated with multidrug resistance (MDR) in several drug-resistant Chinese hamster ovary (CHO) cell lines which exhibit cross-resistance to other unrelated, cytotoxic drugs. In situ hybridization studies (Teeter et al., J. Cell Biol., in press) suggested the presence of an amplified gene associated with the MDR phenotype on the long arm of either of the largest CHO chromosomes (1 or Z1) in vincristine-resistant cells. In this study, somatic cell hybrids were constructed between these vincristine-resistant CHO cells and drug-sensitive murine cells to determine the functional relationship between the chromosome bearing the amplified sequences and the MDR phenotype. Hybrids exhibited primary drug resistance and MDR in an incomplete dominant fashion. Hybrid clones and subclones segregated CHO chromosomes. Concordant segregation between vincristine resistance, the MDR phenotype, the presence of the MDR-associated amplified sequences, overexpression of the gene located in those sequences, and CHO chromosome Z1 was consistent with the hypothesis that there is an amplified gene on chromosome Z1 of the vincristine-resistant CHO cells which is responsible for the MDR in these cells. A low level of discordance between CHO chromosomes Z8 and 2 and the drug resistance phenotype suggests that these chromosomes may contain genes involved with the MDR phenotype.

Human chromosomes control growth of human-chinese hamster somatic cell hybrids

1982 ◽  
Vol 8 (1) ◽  
pp. 105-113 ◽  
Author(s):  
L. J. Donald ◽  
H. S. Wang ◽  
J. L. Hamerton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Control Growth

scholarly journals Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells

1998 ◽  
Vol 21 (2) ◽  
pp. 219-225 ◽  
Author(s):  
Máximo E. Drets ◽  
Marcos Mendizábal
Keyword(s):  
Image Analysis ◽  
Chinese Hamster Ovary ◽  
Computer Graphic ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Ovary Cells ◽  
Normal Human ◽  
Graphic Image ◽  
Chromatin Density

Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.

Genetic effects of chromosomal rearrangements in Chinese hamster ovary cells: expression and chromosomal assignment of TK, GALK, ACP1, ADA, and ITPA loci

1983 ◽  
Vol 3 (11) ◽  
pp. 1967-1974
Author(s):  
R L Stallings ◽  
G M Adair ◽  
J Siciliano ◽  
J Greenspan ◽  
M J Siciliano
Keyword(s):  
Polyethylene Glycol ◽  
Chinese Hamster Ovary ◽  
Gene Deletion ◽  
Chinese Hamster Ovary Cells ◽  
Chromosomal Rearrangements ◽  
Chinese Hamster ◽  
Chromosome 7 ◽  
Chromosomal Assignment ◽  
Somatic Cell Hybrids ◽  
Ovary Cells

Polyethylene glycol-mediated fusion of Chinese hamster ovary (CHO) cells with mouse Cl1D cells produced interspecific somatic cell hybrids which slowly segregated CHO chromosomes. Cytogenetic and isozyme analysis of HAT- and bromodeoxyuridine-selected hybrid subclones and of members of a hybrid clone panel retaining different combinations of CHO chromosomes enabled provisional assignments of the following enzyme loci to CHO chromosomes: TK, GALK, and ACP1 to chromosome 7; TK and GALK to chromosome Z13; ACP1, ADA, and ITPA to chromosome Z8; and ADA and ITPA to chromosome Z9. These genetic markers reflect the origin of each of these Z group chromosomes and indicate the functional activity of alleles located on rearranged chromosomes. Identification of diploid electrophoretic shift mutations for ADA and ITPA was consistent with those observations. Assignment of the functional TK locus in TK+/- CHO-AT3-2 cells indicated that gene deletion may be responsible for TK hemizygosity in this subline.

Multidrug-resistant phenotype cosegregates with an amplified gene in somatic cell hybrids of drug-resistant Chinese hamster ovary cells and drug-sensitive murine cells

1986 ◽  
Vol 6 (12) ◽  
pp. 4268-4273
Author(s):  
L D Teeter ◽  
J A Sanford ◽  
S Sen ◽  
R L Stallings ◽  
M J Siciliano ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Somatic Cell ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Cross Resistance ◽  
Drug Resistant ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Primary Drug Resistance

Gene amplification has been associated with multidrug resistance (MDR) in several drug-resistant Chinese hamster ovary (CHO) cell lines which exhibit cross-resistance to other unrelated, cytotoxic drugs. In situ hybridization studies (Teeter et al., J. Cell Biol., in press) suggested the presence of an amplified gene associated with the MDR phenotype on the long arm of either of the largest CHO chromosomes (1 or Z1) in vincristine-resistant cells. In this study, somatic cell hybrids were constructed between these vincristine-resistant CHO cells and drug-sensitive murine cells to determine the functional relationship between the chromosome bearing the amplified sequences and the MDR phenotype. Hybrids exhibited primary drug resistance and MDR in an incomplete dominant fashion. Hybrid clones and subclones segregated CHO chromosomes. Concordant segregation between vincristine resistance, the MDR phenotype, the presence of the MDR-associated amplified sequences, overexpression of the gene located in those sequences, and CHO chromosome Z1 was consistent with the hypothesis that there is an amplified gene on chromosome Z1 of the vincristine-resistant CHO cells which is responsible for the MDR in these cells. A low level of discordance between CHO chromosomes Z8 and 2 and the drug resistance phenotype suggests that these chromosomes may contain genes involved with the MDR phenotype.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
Sign in / Sign up

Export Citation Format

Share Document