scholarly journals 32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies

Mutagenesis ◽  
2007 ◽  
Vol 22 (6) ◽  
pp. 381-385 ◽  
Author(s):  
A. Munnia ◽  
F. Saletta ◽  
A. Allione ◽  
S. Piro ◽  
M. Confortini ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Aromatic Compound ◽  
Epidemiology Studies ◽  
Labelling Method

scholarly journals Use of short tandem repeat fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies

2014 ◽  
Vol 95 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Victoria C. Edwards ◽  
C. Patrick McClure ◽  
Richard J. P. Brown ◽  
Emma Thompson ◽  
William L. Irving ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Sequence Analysis ◽  
Molecular Epidemiology ◽  
Alternative Explanation ◽  
Sequence Analyses ◽  
Distinct Virus ◽  
Virus Sequence ◽  
Epidemiology Studies ◽  
Short Tandem

Sequence analysis is used to define the molecular epidemiology and evolution of the hepatitis C virus. Whilst most studies have shown that individual patients harbour viruses that are derived from a limited number of highly related strains, some recent reports have shown that some patients can be co-infected with very distinct variants whose frequency can fluctuate greatly. Whilst co-infection with highly divergent strains is possible, an alternative explanation is that such data represent contamination or sample mix-up. In this study, we have shown that DNA fingerprinting techniques can accurately assess sample provenance and differentiate between samples that are truly exhibiting mixed infection from those that harbour distinct virus populations due to sample mix-up. We have argued that this approach should be adopted routinely in virus sequence analyses to validate sample provenance.

Cryopreserving whole blood for functional assays using viable lymphocytes in molecular epidemiology studies

2001 ◽  
Vol 166 (2) ◽  
pp. 155-163 ◽  
Author(s):  
L Cheng ◽  
L.E Wang ◽  
M.R Spitz ◽  
Q Wei
Keyword(s):  
Molecular Epidemiology ◽  
Whole Blood ◽  
Functional Assays ◽  
Epidemiology Studies

scholarly journals Perceptions of molecular epidemiology studies of HIV among stakeholders

2017 ◽  
Vol 6 (3) ◽  
Author(s):  
Cynthia Schairer ◽  
Sanjay R. Mehta ◽  
Staal A. Vinterbo ◽  
Martin Hoenigl ◽  
Michael Kalichman ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Qualitative Interviews ◽  
Public Health Research ◽  
Hiv Care ◽  
Willingness To Participate ◽  
Research Subjects ◽  
Privacy Breaches ◽  
Epidemiology Studies ◽  
Study Participants ◽  
Design And Methods

Background: Advances in viral sequence analysis make it possible to track the spread of infectious pathogens, such as HIV, within a population. When used to study HIV, these analyses (i.e., molecular epidemiology) potentially allow inference of the identity of individual research subjects. Current privacy standards are likely insufficient for this type of public health research. To address this challenge, it will be important to understand how stakeholders feel about the benefits and risks of such research. Design and Methods: To better understand perceived benefits and risks of these research methods, in-depth qualitative interviews were conducted with HIV-infected individuals, individuals at high-risk for contracting HIV, and professionals in HIV care and prevention. To gather additional perspectives, attendees to a public lecture on molecular epidemiology were asked to complete an informal questionnaire. Results: Among those interviewed and polled, there was near unanimous support for using molecular epidemiology to study HIV. Questionnaires showed strong agreement about benefits of molecular epidemiology, but diverse attitudes regarding risks. Interviewees acknowledged several risks, including privacy breaches and provocation of anti-gay sentiment. The interviews also demonstrated a possibility that misunderstandings about molecular epidemiology may affect how risks and benefits are evaluated. Conclusions: While nearly all study participants agree that the benefits of HIV molecular epidemiology outweigh the risks, concerns about privacy must be addressed to ensure continued trust in research institutions and willingness to participate in research.

scholarly journals RESPONSE: Re: Assessing the Probability That a Positive Report is False: An Approach for Molecular Epidemiology Studies

2004 ◽  
Vol 96 (22) ◽  
pp. 1722-1723 ◽  
Author(s):  
S. Wacholder ◽  
S. Chanock ◽  
M. Garcia-Closas ◽  
H. A. Katki ◽  
L. El ghormli ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Positive Report ◽  
Epidemiology Studies

scholarly journals Analysis of the transcriptome in molecular epidemiology studies

2013 ◽  
Vol 54 (7) ◽  
pp. 500-517 ◽  
Author(s):  
Cliona M. McHale ◽  
Luoping Zhang ◽  
Reuben Thomas ◽  
Martyn T. Smith
Keyword(s):  
Molecular Epidemiology ◽  
Epidemiology Studies

P XVI B.8 Immunohistochemical detection of PAH-DNA adducts — An application in molecular epidemiology studies

1997 ◽  
Vol 379 (1) ◽  
pp. S162
Author(s):  
Grażyna Motykiewicz ◽  
Ewa Małusecka ◽  
Paula T.M. Van den Berg ◽  
Jadwiga Michalska ◽  
Robert A. Baan ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Dna Adducts ◽  
Immunohistochemical Detection ◽  
Epidemiology Studies

scholarly journals Metabolism and biomarkers of heterocyclic aromatic amines in humans

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Medjda Bellamri ◽  
Scott J. Walmsley ◽  
Robert J. Turesky
Keyword(s):  
Cancer Risk ◽  
Molecular Epidemiology ◽  
Aromatic Amines ◽  
Heterocyclic Aromatic Amines ◽  
Epidemiology Studies ◽  
The Impact ◽  
Analytical Approaches ◽  
Cooked Meat ◽  
Cooked Meats

AbstractHeterocyclic aromatic amines (HAAs) form during the high-temperature cooking of meats, poultry, and fish. Some HAAs also arise during the combustion of tobacco. HAAs are multisite carcinogens in rodents, inducing cancer of the liver, gastrointestinal tract, pancreas, mammary, and prostate glands. HAAs undergo metabolic activation by N-hydroxylation of the exocyclic amine groups to produce the proposed reactive intermediate, the heteroaryl nitrenium ion, which is the critical metabolite implicated in DNA damage and genotoxicity. Humans efficiently convert HAAs to these reactive intermediates, resulting in HAA protein and DNA adduct formation. Some epidemiologic studies have reported an association between frequent consumption of well-done cooked meats and elevated cancer risk of the colorectum, pancreas, and prostate. However, other studies have reported no associations between cooked meat and these cancer sites. A significant limitation in epidemiology studies assessing the role of HAAs and cooked meat in cancer risk is their reliance on food frequency questionnaires (FFQ) to gauge HAA exposure. FFQs are problematic because of limitations in self-reported dietary history accuracy, and estimating HAA intake formed in cooked meats at the parts-per-billion level is challenging. There is a critical need to establish long-lived biomarkers of HAAs for implementation in molecular epidemiology studies designed to assess the role of HAAs in health risk. This review article highlights the mechanisms of HAA formation, mutagenesis and carcinogenesis, the metabolism of several prominent HAAs, and the impact of critical xenobiotic-metabolizing enzymes on biological effects. The analytical approaches that have successfully biomonitored HAAs and their biomarkers for molecular epidemiology studies are presented.

Sign in / Sign up

Export Citation Format

Share Document