scholarly journals A triple-helix forming oligonucleotide targeting genomic DNA fails to induce mutation

Mutagenesis ◽  
2012 ◽  
Vol 27 (6) ◽  
pp. 713-719 ◽  
Author(s):  
Reshat Reshat ◽  
Catherine C. Priestley ◽  
Nigel J. Gooderham
Keyword(s):  
Genomic Dna ◽  
Triple Helix ◽  
Induce Mutation

scholarly journals Sequence-Specific Triple Helix Formation with Genomic DNA†

Biochemistry ◽  
2007 ◽  
Vol 46 (40) ◽  
pp. 11240-11252 ◽  
Author(s):  
Zhaoyang Ye ◽  
Ramareddy V. Guntaka ◽  
Ram I. Mahato
Keyword(s):  
Genomic Dna ◽  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation

Double strand cleavage of genomic DNA at a single site by triple helix formation

1988 ◽  
Vol 110 (23) ◽  
pp. 7927-7929 ◽  
Author(s):  
Scott A. Strobel ◽  
Heinz E. Moser ◽  
Peter B. Dervan
Keyword(s):  
Genomic Dna ◽  
Triple Helix ◽  
Single Site ◽  
Helix Formation ◽  
Triple Helix Formation

Paris I Dysfibrinogenemia: A Point Mutation in Intron 8 Results in Insertion of a 15 Amino Acid Sequence in the Fibrinogen γ-Chain

1993 ◽  
Vol 69 (03) ◽  
pp. 217-220 ◽  
Author(s):  
Jonathan B Rosenberg ◽  
Peter J Newman ◽  
Michael W Mosesson ◽  
Marie-Claude Guillin ◽  
David L Amrani
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Point Mutation ◽  
Genomic Dna ◽  
Comparative Sequence Analysis ◽  
Chain Gene ◽  
Molecular Defect ◽  
Nucleotide Position ◽  
Normal Individuals ◽  
Polymerase Chain

SummaryParis I dysfibrinogenemia results in the production of a fibrinogen molecule containing a functionally abnormal γ-chain. We determined the basis of the molecular defect using polymerase chain reaction (PCR) to amplify the γ-chain region of the Paris I subject’s genomic DNA. Comparative sequence analysis of cloned PCR segments of normal and Paris I genomic DNA revealed only an A→G point mutation occurring at nucleotide position 6588 within intron 8 of the Paris I γ-chain gene. We examined six normal individuals and found only normal sequence in this region, indicating that this change is not likely to represent a normal polymorphism. This nucleotide change leads to a 45 bp fragment being inserted between exons 8 and 9 in the mature γparis I chain mRNA, and encodes a 15 amino acid insert after γ350 [M-C-G-E-A-L-P-M-L-K-D-P-C-Y]. Alternative splicing of this region from intron 8 into the mature Paris I γ-chain mRNA also results after translation into a substitution of S for G at position γ351. Biochemical studies of 14C-iodoacetamide incorporation into disulfide-reduced Paris I and normal fibrinogen corroborated the molecular biologic predictions that two additional cysteine residues exist within the γpariS I chain. We conclude that the insertion of this amino acid sequence leads to a conformationallyaltered, and dysfunctional γ-chain in Paris I fibrinogen.

“Triple Helix” in Russia’s Innovation System

2007 ◽  
pp. 123-135 ◽  
Author(s):  
I. Dezhina ◽  
V. Kiseleva
Keyword(s):  
Triple Helix ◽  
Government Regulation ◽  
Innovation System ◽  
Innovation Systems ◽  
Modern Theory

The article analyzes modern theory that explains the specificity of relationships among government, science and business in innovation systems - the "triple helix" concept. Factors that determine the appearance of new theory are systematized. The peculiarities of formation of "triple helix" in Russia are described, including the development of science, business and the system of government regulation in innovation sphere. The conclusion is made that currently in Russia only double linkages are formed.

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