Rapid and Competent Protocol for Isolation of Genomic DNA from Vigna Species Suitable for RAPD and Restriction Digestion

2009 ◽  
Vol 1 (3) ◽  
Author(s):  
K Choudhary ◽  
M. Singh ◽  
O. P. Choudhary ◽  
U. Pillai
Keyword(s):  
Genomic Dna ◽  
Restriction Digestion

scholarly journals Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

2005 ◽  
Vol 48 (4) ◽  
pp. 511-521 ◽  
Author(s):  
Leandro Eugênio Cardamoni Diniz ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Fabrício Medeiros Torres ◽  
Eduardo Augusto Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Variability ◽  
Clustering Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Restriction Digestion ◽  
Agronomic Features

The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.

scholarly journals Correlation of Phylogenetic Lineages of Group B Streptococci, Identified by Analysis of Restriction‐Digestion Patterns of Genomic DNA, withinfBAlleles and Mobile Genetic Elements

2002 ◽  
Vol 186 (7) ◽  
pp. 1034-1038 ◽  
Author(s):  
Shinji Takahashi ◽  
Shauna Detrick ◽  
April A. Whiting ◽  
Anne J. Blaschke‐Bonkowksy ◽  
Youko Aoyagi ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Mobile Genetic Elements ◽  
Restriction Digestion ◽  
Group B Streptococci ◽  
Genetic Elements ◽  
Phylogenetic Lineages ◽  
Group B

scholarly journals DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Sunil Kumar Sahu ◽  
Muthusamy Thangaraj ◽  
Kandasamy Kathiresan
Keyword(s):  
Salt Marsh ◽  
Secondary Metabolites ◽  
Liquid Nitrogen ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Wide Spectrum ◽  
Pcr Amplification ◽  
Restriction Digestion ◽  
High Quality ◽  
Salt Marsh Species

Mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA. Although a plethora of plant DNA isolation protocols exist, extracting DNA from mangroves and salt marsh species is a challenging task. This study describes a rapid and reliable cetyl trimethylammonium bromide (CTAB) protocol suited specifically for extracting DNA from plants which are rich in polysaccharides and secondary metabolites, and the protocol also excludes the use of expensive liquid nitrogen and toxic phenols. Purity of extracted DNA was excellent as evident by A260/A280 ratio ranging from 1.78 to 1.84 and A260/A230 ratio was >2, which also suggested that the preparations were sufficiently free of proteins and polyphenolics/polysaccharide compounds. DNA concentration ranged from 8.8 to 9.9 μg μL−1. The extracted DNA was amenable to RAPD, restriction digestion, and PCR amplification of plant barcode genes (matK and rbcl). The optimized method is suitable for both dry and fresh leaves. The success of this method in obtaining high-quality genomic DNA demonstrated the broad applicability of this method.

Paris I Dysfibrinogenemia: A Point Mutation in Intron 8 Results in Insertion of a 15 Amino Acid Sequence in the Fibrinogen γ-Chain

1993 ◽  
Vol 69 (03) ◽  
pp. 217-220 ◽  
Author(s):  
Jonathan B Rosenberg ◽  
Peter J Newman ◽  
Michael W Mosesson ◽  
Marie-Claude Guillin ◽  
David L Amrani
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Point Mutation ◽  
Genomic Dna ◽  
Comparative Sequence Analysis ◽  
Chain Gene ◽  
Molecular Defect ◽  
Nucleotide Position ◽  
Normal Individuals ◽  
Polymerase Chain

SummaryParis I dysfibrinogenemia results in the production of a fibrinogen molecule containing a functionally abnormal γ-chain. We determined the basis of the molecular defect using polymerase chain reaction (PCR) to amplify the γ-chain region of the Paris I subject’s genomic DNA. Comparative sequence analysis of cloned PCR segments of normal and Paris I genomic DNA revealed only an A→G point mutation occurring at nucleotide position 6588 within intron 8 of the Paris I γ-chain gene. We examined six normal individuals and found only normal sequence in this region, indicating that this change is not likely to represent a normal polymorphism. This nucleotide change leads to a 45 bp fragment being inserted between exons 8 and 9 in the mature γparis I chain mRNA, and encodes a 15 amino acid insert after γ350 [M-C-G-E-A-L-P-M-L-K-D-P-C-Y]. Alternative splicing of this region from intron 8 into the mature Paris I γ-chain mRNA also results after translation into a substitution of S for G at position γ351. Biochemical studies of 14C-iodoacetamide incorporation into disulfide-reduced Paris I and normal fibrinogen corroborated the molecular biologic predictions that two additional cysteine residues exist within the γpariS I chain. We conclude that the insertion of this amino acid sequence leads to a conformationallyaltered, and dysfunctional γ-chain in Paris I fibrinogen.

Genomic Dna Isolation From Plumbago L. Species Growing In India

2012 ◽  
Vol 2 (7) ◽  
pp. 45-46
Author(s):  
Varsha N Nathar ◽  
◽  
Prashant J Gadge
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

Susceptibility to insulin-dependent diabetes defined by restriction enzyme polymorphism of HLA-D region genomic DNA

Diabetes ◽  
1984 ◽  
Vol 33 (10) ◽  
pp. 958-965 ◽  
Author(s):  
D. Owerbach ◽  
B. Hagglof ◽  
A. Lernmark ◽  
G. Holmgren
Keyword(s):  
Restriction Enzyme ◽  
Genomic Dna ◽  
Enzyme Polymorphism ◽  
Insulin Dependent Diabetes ◽  
D Region ◽  
Insulin Dependent
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