scholarly journals Nucleotide sequence of the spinach chloroplast 4.5S ribosomal RNA gene and of its 5' flanking region including the 3' end of the 23S rRNA gene

1986 ◽  
Vol 14 (23) ◽  
pp. 9533-9533 ◽  
Author(s):  
Hélène Audren ◽  
Régis Mache
Keyword(s):  
Nucleotide Sequence ◽  
Ribosomal Rna ◽  
23S Rrna ◽  
Rrna Gene ◽  
Spinach Chloroplast ◽  
Ribosomal Rna Gene ◽  
23S Rrna Gene ◽  
Flanking Region

Nature of the genome of the saprophytic spirochete Spirochaeta aurantia and its ribosomal RNA operons

2004 ◽  
Vol 50 (11) ◽  
pp. 967-971 ◽  
Author(s):  
Richard McLaughlin ◽  
David M Secko ◽  
Catherine J Paul ◽  
Andrew M Kropinski
Keyword(s):  
Ribosomal Rna ◽  
Gel Electrophoresis ◽  
Large Subunit ◽  
Complete Sequence ◽  
Pulsed Field Gel Electrophoresis ◽  
23S Rrna ◽  
Rrna Gene ◽  
Pulsed Field ◽  
23S Rrna Gene ◽  
Intergenic Regions

Using restriction endonucleases DraI, AseI, and I-CeuI in conjunction with pulsed-field gel electrophoresis, we have shown that Spirochaeta aurantia M1 possesses a circular 3.98-Mb genome. This is the second largest spirochete chromosome yet analyzed. The observation that the latter enzyme cuts in 3 places suggests the presence of 3 copies of the large subunit (23S) rRNA gene (rrl), which was confirmed by Southern hybridizations. The complete sequence of 2 of the ribosomal RNA operons was determined, revealing that their structure resembled that of the typical member of the bacterial superkingdom: rrs (16S; 1561 bp), tRNA, rrl (23S; 2972 bp), and rrf (5S; 110 bp). The S. aurantia rrs–rrl intergenic regions, as with Treponema denticola, contain genes specifying a 73-nt tRNAAla (anticodon TGC) and a 77-nt tRNAIle (anticodon GAT).Key words: spirochete, pulsed-field gel electrophoresis, ribosomal RNA, operon, Spirochaeta aurantia.

scholarly journals Nucleotide sequence of theEuglena gracilischloroplast 23S rRNA gene of therrnCoperon

1988 ◽  
Vol 16 (19) ◽  
pp. 9340-9340 ◽  
Author(s):  
Gloria M. Yepiz-Plascencia ◽  
Michael E. Jenkins ◽  
Richard B. Hallick
Keyword(s):  
Nucleotide Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

scholarly journals Phylogeny and nucleotide sequence of a 23S rRNA gene fromNeisseria gonorrhoeaeandNeisseria meningitidis

1992 ◽  
Vol 20 (17) ◽  
pp. 4657-4657 ◽  
Author(s):  
Karin Wolff ◽  
Sabine Sperka ◽  
Anne Stern
Keyword(s):  
Nucleotide Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

Nucleotide sequence of the chloroplast 23S rRNA gene from alder (Alnus incana)

1992 ◽  
Vol 18 (3) ◽  
pp. 601-602
Author(s):  
Madeleine L�vesque ◽  
Douglas A. Johnson
Keyword(s):  
Nucleotide Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
Alnus Incana ◽  
23S Rrna Gene

scholarly journals Molecular pathways to high-level azithromycin resistance in Neisseria gonorrhoeae

2021 ◽  
Author(s):  
J G E Laumen ◽  
S S Manoharan-Basil ◽  
E Verhoeven ◽  
S Abdellati ◽  
I De Baetselier ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Ribosomal Proteins ◽  
Efflux Pump ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Evolution Of Resistance ◽  
High Level ◽  
Azithromycin Resistance ◽  
Level Resistance

Abstract Background The prevalence of azithromycin resistance in Neisseria gonorrhoeae is increasing in numerous populations worldwide. Objectives To characterize the genetic pathways leading to high-level azithromycin resistance. Methods A customized morbidostat was used to subject two N. gonorrhoeae reference strains (WHO-F and WHO-X) to dynamically sustained azithromycin pressure. We tracked stepwise evolution of resistance by whole genome sequencing. Results Within 26 days, all cultures evolved high-level azithromycin resistance. Typically, the first step towards resistance was found in transitory mutations in genes rplD, rplV and rpmH (encoding the ribosomal proteins L4, L22 and L34 respectively), followed by mutations in the MtrCDE-encoded efflux pump and the 23S rRNA gene. Low- to high-level resistance was associated with mutations in the ribosomal proteins and MtrCDE efflux pump. However, high-level resistance was consistently associated with mutations in the 23S ribosomal RNA, mainly the well-known A2059G and C2611T mutations, but also at position A2058G. Conclusions This study enabled us to track previously reported mutations and identify novel mutations in ribosomal proteins (L4, L22 and L34) that may play a role in the genesis of azithromycin resistance in N. gonorrhoeae.

scholarly journals Molecular characterization of a ceftriaxone-resistant Neisseria gonorrhoeae strain found in Switzerland: a case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Konrad Egli ◽  
Anna Roditscheff ◽  
Ursula Flückiger ◽  
Martin Risch ◽  
Lorenz Risch ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
23S Rrna ◽  
Rrna Gene ◽  
Limited Information ◽  
Specific Pcr ◽  
Appropriate Treatment ◽  
23S Rrna Gene ◽  
Allele Type ◽  
Ciprofloxacin Resistance

Abstract Background The resistance of Neisseria gonorrhoeae to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in Neisseria gonorrhoeae (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different β-lactams and fluoroquinolones. Case presentation A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (penA, ponA, porinB, mtr, gyrA, parC, 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the porinB gene were observed, and data on this gene are limited. Information on the identified allele type of the penA gene is very limited as well. Three different mutations of parC and gyrA that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance. Conclusion The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.

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