scholarly journals Calf thymus DNA polymerase δ: purification, biochemical and functional properties of the enzyme after its separation from DNA polymerase α, a DNA dependent ATPase and proliferating cell nuclear antigen

1988 ◽  
Vol 16 (14) ◽  
pp. 6279-6295 ◽  
Author(s):  
Federico Focher ◽  
Silvio Spadari ◽  
Barbara Ginelli ◽  
Michael Hottiger ◽  
Max Gassmann ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Functional Properties ◽  
Proliferating Cell Nuclear Antigen ◽  
Calf Thymus Dna ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Dna Polymerase Α ◽  
Dependent Atpase ◽  
Calf Thymus ◽  
Proliferating Cell

scholarly journals Calf thymus DNA polymerase δ Independent of proliferating cell nuclear antigen (PCNA)

1989 ◽  
Vol 17 (5) ◽  
pp. 1805-1821 ◽  
Author(s):  
Federico Focher ◽  
Max Gassmann ◽  
Peter Hafkmeyer ◽  
Elena Ferrari ◽  
Silvio Spadari ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Calf Thymus Dna ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Calf Thymus ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen Promotes Misincorporation Catalyzed by Calf Thymus DNA Polymerase δ

1996 ◽  
Vol 271 (49) ◽  
pp. 31711-31717 ◽  
Author(s):  
Dmitry Ju. Mozzherin ◽  
Maeve McConnell ◽  
Maxim V. Jasko ◽  
Alexander A. Krayevsky ◽  
Cheng-Keat Tan ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Calf Thymus Dna ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Calf Thymus ◽  
Proliferating Cell

scholarly journals Identification of a Novel Protein, PDIP38, That Interacts with the p50 Subunit of DNA Polymerase δ and Proliferating Cell Nuclear Antigen

2003 ◽  
Vol 278 (12) ◽  
pp. 10041-10047 ◽  
Author(s):  
Li Liu ◽  
Esther M. Rodriguez-Belmonte ◽  
Nayef Mazloum ◽  
Bin Xie ◽  
Marietta Y. W. T. Lee
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Proliferating Cell ◽  
Novel Protein

scholarly journals Direct Interaction of Proliferating Cell Nuclear Antigen with the Small Subunit of DNA Polymerase δ

2002 ◽  
Vol 277 (27) ◽  
pp. 24340-24345 ◽  
Author(s):  
Xiaoqing Lu ◽  
Cheng-Keat Tan ◽  
Jin-Qiu Zhou ◽  
Min You ◽  
L. Michael Carastro ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Direct Interaction ◽  
Small Subunit ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Proliferating Cell

scholarly journals Identification of PCNA-interacting protein motifs in human DNA polymerase δ

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Prashant Khandagale ◽  
Shweta Thakur ◽  
Narottam Acharya
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Protein Motif ◽  
Interacting Protein ◽  
Protein Motifs ◽  
Dna Polymerase Δ ◽  
Human Dna ◽  
Processivity Factor ◽  
Proliferating Cell

Abstract DNA polymerase δ (Polδ) is a highly processive essential replicative DNA polymerase. In humans, the Polδ holoenzyme consists of p125, p50, p68 and p12 subunits and recently, we showed that the p12 subunit exists as a dimer. Extensive biochemical studies suggest that all the subunits of Polδ interact with the processivity factor proliferating cell nuclear antigen (PCNA) to carry out a pivotal role in genomic DNA replication. While PCNA-interacting protein motif (PIP) motifs in p68, p50 and p12 have been mapped, same in p125, the catalytic subunit of the holoenzyme, remains elusive. Therefore, in the present study by using multiple approaches we have conclusively mapped a non-canonical PIP motif from residues 999VGGLLAFA1008 in p125, which binds to the inter-domain-connecting loop (IDCL) of PCNA with high affinity. Collectively, including previous studies, we conclude that similar to Saccharomyces cerevisiae Polδ, each of the human Polδ subunits possesses motif to interact with PCNA and significantly contributes toward the processive nature of this replicative DNA polymerase.

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