Repair of rDNA in Saccharomyces cerevisiae: RAD4-independent strand- specific nucleotide excision repair of RNA polymerase I transcribed genes

Nucleotide excision repair (NER) guarantees genome integrity against UV light-induced DNA damage. After UV irradiation, cells have to cope with a general transcriptional block. To ensure UV lesions repair specifically on transcribed genes, NER is coupled with transcription in an extremely organized pathway known as transcription-coupled repair. In highly metabolic cells, more than 60% of total cellular transcription results from RNA polymerase I activity. Repair of the mammalian transcribed ribosomal DNA has been scarcely studied. UV lesions severely block RNA polymerase I activity and the full transcription-coupled repair machinery corrects damage on actively transcribed ribosomal DNAs. After UV irradiation, RNA polymerase I is more bound to the ribosomal DNA and both are displaced to the nucleolar periphery. Importantly, the reentry of RNA polymerase I and the ribosomal DNA is dependent on the presence of UV lesions on DNA and independent of transcription restart.

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RNA Polymerase-I-Dependent Transcription-coupled Nucleotide Excision Repair of UV-Induced DNA Lesions at Transcription Termination Sites, inSaccharomyces cerevisiae

Photochemistry and Photobiology ◽

10.1111/php.12690 ◽

2017 ◽

Vol 93 (1) ◽

pp. 363-374 ◽

Cited By ~ 1

Author(s):

François Peyresaubes ◽

Carlos Zeledon ◽

Laetitia Guintini ◽

Romain Charton ◽

Alexia Muguet ◽

...

Keyword(s):

Rna Polymerase ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Transcription Termination ◽

Dna Lesions ◽

Rna Polymerase I ◽

Nucleotide Excision ◽

Polymerase I

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Nucleotide excision repair in the yeast Saccharomyces cerevisiae : its relationship to specialized mitotic recombination and RNA polymerase II basal transcription

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.1995.0010 ◽

1995 ◽

Vol 347 (1319) ◽

pp. 63-68 ◽

Cited By ~ 27

Keyword(s):

Saccharomyces Cerevisiae ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Gene Products ◽

Basal Transcription ◽

Yeast Saccharomyces Cerevisiae ◽

Nucleotide Excision ◽

Yeast Genes

Nucleotide excision repair (ner) in eukaryotes is a biochemically complex process involving multiple gene products. The budding yeast Saccharomyces cerevisiae is an informative model for this process. Multiple genes and in some cases gene products that are indispensable for ner have been isolated from this organism. Homologues of many of these yeast genes are structurally and functionally conserved in higher organisms, including humans. The yeast Rad1/Rad10 heterodimeric protein complex is an endonuclease that is believed to participate in damage-specific incision of DNA during ner . This endonuclease is also required for specialized types of recombination. The products of the RAD3, SSL2(RAD25) SSL1 and TFB1 genes have dual roles in ner and in RNA polymerase II-dependent basal transcription.

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