scholarly journals A peripheral element assembles the compact core structure essential for group I intron self-splicing

2005 ◽  
Vol 33 (14) ◽  
pp. 4602-4611 ◽  
Author(s):  
M. Xiao
1993 ◽  
Vol 21 (2) ◽  
pp. 311-317 ◽  
Author(s):  
Barbara Striecjer ◽  
Uwe von Ahsen ◽  
Renée Schroeder

2009 ◽  
Vol 191 (12) ◽  
pp. 4044-4046 ◽  
Author(s):  
Rahul Raghavan ◽  
Linda D. Hicks ◽  
Michael F. Minnick

ABSTRACT Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3′-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.


Nature ◽  
2004 ◽  
Vol 430 (6995) ◽  
pp. 45-50 ◽  
Author(s):  
Peter L. Adams ◽  
Mary R. Stahley ◽  
Anne B. Kosek ◽  
Jimin Wang ◽  
Scott A. Strobel

2004 ◽  
Vol 186 (23) ◽  
pp. 8153-8155 ◽  
Author(s):  
Richard P. Bonocora ◽  
David A. Shub

ABSTRACT Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I intron in the coding sequence of the DNA polymerase genes of ΦI and W31, phages that are closely related to T7. The introns belong to subgroup IA2 and both contain an open reading frame, inserted into structural element P6a, encoding a protein belonging to the HNH family of homing endonucleases. The introns splice efficiently in vivo and self-splice in vitro under mild conditions of ionic strength and temperature. We conclude that there is no barrier for maintenance of group I introns in phages of proteobacteria.


ChemBioChem ◽  
2004 ◽  
Vol 5 (12) ◽  
pp. 1647-1652 ◽  
Author(s):  
Matthew D. Disney ◽  
Jessica L. Childs ◽  
Douglas H. Turner

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