scholarly journals The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A)

2017 ◽  
pp. gkx002 ◽  
Author(s):  
Jędrzej Małecki ◽  
Vinay Kumar Aileni ◽  
Angela Y.Y. Ho ◽  
Juliane Schwarz ◽  
Anders Moen ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Mrna Translation ◽  
The Novel ◽  
Eukaryotic Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

scholarly journals Eukaryotic Elongation Factor 1A2 Cooperates with Phosphatidylinositol-4 Kinase III β To Stimulate Production of Filopodia through Increased Phosphatidylinositol-4,5 Bisphosphate Generation

2008 ◽  
Vol 28 (14) ◽  
pp. 4549-4561 ◽  
Author(s):  
Sujeeve Jeganathan ◽  
Anne Morrow ◽  
Anahita Amiri ◽  
Jonathan M. Lee
Keyword(s):  
Elongation Factor ◽  
Actin Remodeling ◽  
Cellular Processes ◽  
Eukaryotic Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Membrane Bound ◽  
Elongation Factor 1A2 ◽  
Phosphatidylinositol 4 ◽  
Elongation Factor 1 ◽  
Transforming Gene

ABSTRACT Eukaryotic elongation factor 1 alpha 2 (eEF1A2) is a transforming gene product that is highly expressed in human tumors of the ovary, lung, and breast. eEF1A2 also stimulates actin remodeling, and the expression of this factor is sufficient to induce the formation of filopodia, long cellular processes composed of bundles of parallel actin filaments. Here, we find that eEF1A2 stimulates formation of filopodia by increasing the cellular abundance of cytosolic and plasma membrane-bound phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2]. We have previously reported that the eEF1A2 protein binds and activates phosphatidylinositol-4 kinase III beta (PI4KIIIβ), and we find that production of eEF1A2-dependent PI(4,5)P2 and generation of filopodia require PI4KIIIβ. Furthermore, PI4KIIIβ is itself capable of activating both the production of PI(4,5)P2 and the creation of filopodia. We propose a model for extrusion of filopodia in which eEF1A2 activates PI4KIIIβ, and activated PI4KIIIβ stimulates production of PI(4,5)P2 and filopodia by increasing PI4P abundance. Our work suggests an important role for both eEF1A2 and PI4KIIIβ in the control of PI(4,5)P2 signaling and actin remodeling.

scholarly journals Localization of S1 and elongation factor-1 alpha mRNA in rat brain and liver by non-radioactive in situ hybridization.

1993 ◽  
Vol 41 (7) ◽  
pp. 1093-1098 ◽  
Author(s):  
S Lee ◽  
E Stollar ◽  
E Wang
Keyword(s):  
In Situ Hybridization ◽  
Elongation Factor ◽  
Mrna Translation ◽  
Northern Analysis ◽  
Specific Gene ◽  
Rnase Protection ◽  
Rat Brain And Liver ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Elongation factor-1 alpha (EF-1 alpha) is a ubiquitous, highly conserved protein that functions in peptide elongation during mRNA translation. We recently reported that, as do lower species, mammals also contain a second EF-1 alpha-like gene (S1). Unlike EF-1 alpha, which is present in all tissues, S1 mRNA is detected only in brain, heart, and muscle by Northern analysis and RNAse protection assays. In this report we present the identification of S1 and EF-1 alpha messages by non-radioactive in situ hybridization in brain and liver. We show that with this technique we can detected S1 mRNA only in certain cells in brain, mostly neurons; on the other hand, EF-1 alpha is present in all cell types that we have studied so far. We demonstrate that although EF-1 alpha mRNA can be detected in S1-negative cells it is also present in high abundance in S1-positive cells. The results presented here correlate with our previous finding that mammalian species contain a tissue-specific EF-1 alpha-like gene, S1. The presence of a second EF-1 alpha-like transcript within fully differentiated cells suggests a novel cell type-specific gene expression whose function may be related to the permanent growth-arrested state of cells in brain, heart, and muscle.

scholarly journals Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.

1985 ◽  
Vol 260 (5) ◽  
pp. 3090-3096
Author(s):  
P Cottrelle ◽  
D Thiele ◽  
V L Price ◽  
S Memet ◽  
J Y Micouin ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1
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