Human sperm—zona pellucida binding, sperm characteristics and in-vitro fertilization

1989 ◽  
Vol 4 (6) ◽  
pp. 696-701 ◽  
Author(s):  
D.Y. Liu ◽  
A. Lopata ◽  
W.I.H. Johnston ◽  
H.W.G. Baker
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Characteristics

Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽  
2000 ◽  
pp. 15-23 ◽  
Author(s):  
K Jewgenow ◽  
M Rohleder ◽  
I Wegner
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Antigenic Determinants ◽  
Vitro Fertilization ◽  
Contraceptive Vaccine ◽  
Sperm Binding ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

scholarly journals Differential influence of ampullary and isthmic derived epithelial cells on zona pellucida hardening and in vitro fertilization in ovine

2016 ◽  
Vol 16 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Navid Dadashpour Davachi ◽  
Ahmad Zare Shahneh ◽  
Hamid Kohram ◽  
Mahdi Zhandi ◽  
Helia Shamsi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Epithelial Cells ◽  
Zona Pellucida ◽  
Vitro Fertilization

Quality control in an in-vitro fertilization laboratory: use of human sperm survival studies

1989 ◽  
Vol 4 (5) ◽  
pp. 545-549 ◽  
Author(s):  
J. Diane Critchlow ◽  
Phillip L. Matson ◽  
C. Newman Maureen ◽  
Gregory Horne ◽  
Stephen A. Troup ◽  
...  
Keyword(s):  
Quality Control ◽  
In Vitro Fertilization ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Survival ◽  
Survival Studies

Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF)

1986 ◽  
Vol 74 (4) ◽  
pp. 441-443 ◽  
Author(s):  
H. Schmiady ◽  
K. Sperling ◽  
H. Kentenich ◽  
M. Stauber
Keyword(s):  
In Vitro Fertilization ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Chromosomes ◽  
Human Sperm Chromosomes

Dynamics of human sperm acrosome reaction: relation with in vitro fertilization

1991 ◽  
Vol 55 (5) ◽  
pp. 994-999 ◽  
Author(s):  
Patrick Fénichel ◽  
Michèle Donzeau ◽  
Dariush Farahifar ◽  
Bernard Basteris ◽  
Noël Ayraud ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

2013 ◽  
Vol 16 (4) ◽  
pp. 773-785 ◽  
Author(s):  
K. Lasiene ◽  
V. Gedrimas ◽  
A. Vitkus ◽  
S. Glinskyte ◽  
V. Lasys ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Sperm Quality ◽  
Human Sperm ◽  
Developmental Competence ◽  
Vitro Fertilization ◽  
Abnormal Sperm ◽  
Morphological Criteria ◽  
Negative Effect

Abstract The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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