Pyrrolidone Carboxyl Peptidase from the Hyperthermophilic Archaeon Pyrococcus furiosus: Cloning and Overexpression in Escherichia coli of the Gene, and Its Application to Protein Sequence Analysis

1998 ◽  
Vol 124 (4) ◽  
pp. 778-783 ◽  
Author(s):  
S. Tsunasawa ◽  
S. Nakura ◽  
T. Tanigawa ◽  
I. Kato
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Protein Sequence ◽  
Pyrococcus Furiosus ◽  
Protein Sequence Analysis ◽  
Hyperthermophilic Archaeon

Chemical Methods for Protein Sequence Analysis

2007 ◽  
pp. 141-158
Author(s):  
Friedrich Lottspeich ◽  
Tony Houthaeve ◽  
Roland Kellner
Keyword(s):  
Sequence Analysis ◽  
Protein Sequence ◽  
Protein Sequence Analysis ◽  
Chemical Methods

scholarly journals A Novel DNA Polymerase Family Found inArchaea

1998 ◽  
Vol 180 (8) ◽  
pp. 2232-2236 ◽  
Author(s):  
Yoshizumi Ishino ◽  
Kayoko Komori ◽  
Isaac K. O. Cann ◽  
Yosuke Koga
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Pyrococcus Furiosus ◽  
Dna Polymerases ◽  
Open Reading Frames ◽  
Gene Products ◽  
Polymerase Gene ◽  
Hyperthermophilic Archaeon ◽  
Dna Polymerase Ii ◽  
Reading Frames

ABSTRACT One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeonPyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499–512, 1997). The genes in M. jannaschiiencoding the proteins that are homologous to the DNA polymerase II ofP. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia colihad both DNA polymerizing and 3′→5′ exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

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