Anaerobic Purification and Characterization of Nitrous Oxide Reductase from Rhodobacter sphaeroides f. sp. denitrificans IL106

1999 ◽  
Vol 125 (5) ◽  
pp. 864-868 ◽  
Author(s):  
K. Sato ◽  
A. Okubo ◽  
S. Yamazaki
Keyword(s):  
Nitrous Oxide ◽  
Rhodobacter Sphaeroides ◽  
Nitrous Oxide Reductase ◽  
Purification And Characterization

Expression, purification, and characterization of NosL, a novel Cu(I) protein of the nitrous oxide reductase (nos) gene cluster

2001 ◽  
Vol 6 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Michele A. McGuirl ◽  
John A. Bollinger ◽  
Nathaniel Cosper ◽  
Robert A. Scott ◽  
David M. Dooley
Keyword(s):  
Nitrous Oxide ◽  
Gene Cluster ◽  
Nitrous Oxide Reductase ◽  
Purification And Characterization ◽  
Nos Gene

scholarly journals Nitrite and Nitrous Oxide Reductase Regulation by Nitrogen Oxides in Rhodobacter sphaeroides f. sp.denitrificans IL106

1999 ◽  
Vol 181 (19) ◽  
pp. 6028-6032 ◽  
Author(s):  
Monique Sabaty ◽  
Carole Schwintner ◽  
Sandrine Cahors ◽  
Pierre Richaud ◽  
Andre Verméglio
Keyword(s):  
Nitrous Oxide ◽  
Nitrate Reductase ◽  
Rhodobacter Sphaeroides ◽  
Type Strain ◽  
Biochemical Analysis ◽  
Wild Type Strain ◽  
Nitrous Oxide Reductase ◽  
Wild Type ◽  
Genes Encoding ◽  
Membrane Bound

ABSTRACT We have cloned the nap locus encoding the periplasmic nitrate reductase in Rhodobacter sphaeroides f. sp.denitrificans IL106. A mutant with this enzyme deleted is unable to grow under denitrifying conditions. Biochemical analysis of this mutant shows that in contrast to the wild-type strain, the level of synthesis of the nitrite and N2O reductases is not increased by the addition of nitrate. Growth under denitrifying conditions and induction of N oxide reductase synthesis are both restored by the presence of a plasmid containing the genes encoding the nitrate reductase. This demonstrates that R. sphaeroides f. sp. denitrificans IL106 does not possess an efficient membrane-bound nitrate reductase and that nitrate is not the direct inducer for the nitrite and N2O reductases in this species. In contrast, we show that nitrite induces the synthesis of the nitrate reductase.

Purification and characterization of Rhodobacter sphaeroides acyl carrier protein

Biochemistry ◽  
1987 ◽  
Vol 26 (10) ◽  
pp. 2740-2746 ◽  
Author(s):  
Cynthia L. Cooper ◽  
Stephen G. Boyce ◽  
Donald R. Lueking
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Purification And Characterization
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