scholarly journals Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies

2001 ◽  
Vol 14 (8) ◽  
pp. 573-582 ◽  
Author(s):  
Jennifer J. Gesell ◽  
Dingjiang Liu ◽  
Vincent S. Madison ◽  
Thomas Hesson ◽  
Yu-Sen Wang ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Drug Discovery ◽  
Rna Helicase ◽  
Mechanistic Studies ◽  
Purification And Characterization ◽  
High Level Expression ◽  
High Level

scholarly journals PURIFICATION AND CHARACTERIZATION OF POLYSACCHARIDE FROM MICROALGAE BTM 11 AS INHIBITOR OF HEPATITIS C VIRUS RNA HELICASE

2015 ◽  
Vol 25 (3) ◽  
pp. 134
Author(s):  
Apon Zaenal Mustopa ◽  
Aksar Chair Lages ◽  
Muhammad Ridwan ◽  
Linda Sukmarini ◽  
Dwi Susilaningsih ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Helicase ◽  
Purification And Characterization ◽  
Hepatitis C Virus Rna ◽  
Virus Rna

scholarly journals Characterization of Hepatitis C Virus Deletion Mutants Circulating in Chronically Infected Patients

2007 ◽  
Vol 81 (22) ◽  
pp. 12496-12503 ◽  
Author(s):  
Suwanna Noppornpanth ◽  
Saskia L. Smits ◽  
Truong Xuan Lien ◽  
Yong Poovorawan ◽  
Albert D. M. E. Osterhaus ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Rna ◽  
Deletion Mutants ◽  
Genotype 3 ◽  
Long Distance ◽  
Bona Fide ◽  
Chronic Hcv ◽  
High Level

ABSTRACT Hepatitis C virus (HCV) has a linear positive-stranded RNA genome of ∼9,600 nucleotides in length and displays a high level of sequence diversity caused by high mutation rates and recombination. However, when we performed long distance reverse transcription-PCRs on HCV RNA isolated from serum of chronic HCV patients, not only full-length HCV genomes but also HCV RNAs which varied in size from 7,600 to 8,346 nucleotides and contained large in-frame deletions between E1 and NS2 were amplified. Carefully designed control experiments indicated that these deletion mutants are a bona fide natural RNA species, most likely packaged in virions. Moreover, deletion mutants were detected in sera of patients infected with different HCV genotypes. We observed that 7/37 (18.9%) of genotype 1, 5/43 (11.6%) of genotype 3, and 4/13 (30.7%) of genotype 6 samples contained HCV deletion mutant genomes. These observations further exemplify HCV's huge genetic diversity and warrant studies to explore their biological relevance.

Purification and characterization of hepatitis C virus non-structural protein 5A expressed in Escherichia coli

2004 ◽  
Vol 37 (1) ◽  
pp. 144-153 ◽  
Author(s):  
Luyun Huang ◽  
Elena V Sineva ◽  
Michele R.S Hargittai ◽  
Suresh D Sharma ◽  
Mehul Suthar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Structural Protein ◽  
Purification And Characterization
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