scholarly journals Trichostatin A Enhances Gap Junctional Intercellular Communication in Primary Cultures of Adult Rat Hepatocytes

2006 ◽  
Vol 91 (2) ◽  
pp. 484-492 ◽  
Author(s):  
Mathieu Vinken ◽  
Tom Henkens ◽  
Tamara Vanhaecke ◽  
Peggy Papeleu ◽  
Albert Geerts ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Trichostatin A ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Gap Junctional Intercellular Communication ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Gap Junctional

Reappearance and long-term maintenance of connexin32 in proliferated adult rat hepatocytes: use of serum-free L-15 medium supplemented with EGF and DMSO

1995 ◽  
Vol 108 (4) ◽  
pp. 1347-1357
Author(s):  
T. Kojima ◽  
T. Mitaka ◽  
D.L. Paul ◽  
M. Mori ◽  
Y. Mochizuki
Keyword(s):  
Intercellular Communication ◽  
Cell Membranes ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Gap Junctional Intercellular Communication ◽  
Serum Free ◽  
Adult Rat ◽  
Junctional Communication ◽  
Adult Rat Hepatocytes ◽  
Gap Junctional

Intercellular communication, especially gap junctional communication, is thought to be one of the highly differentiated functions of hepatocytes. In primary cultures of rat hepatocytes, it has been considered that the maintenance and the reinduction of differentiated functions is very difficult. In the present study, we succeeded in inducing the gap junctional protein connexin32 (Cx32) in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor (EGF) and dimethylsulfoxide (DMSO). When the hepatocytes were cultured in L-15 medium supplemented with 20 mM NaHCO3 and 10 ng/ml EGF in a 5% CO2:95% air incubator, the cells proliferated. Fluorescence immunocytochemistry showed spots immunoreactive to Cx32 on the cell membranes between adjacent cells until day 3, but only a few Cx32-positive spots were found after day 4. Western and northern blot analyses also showed that the amounts of both the protein and mRNA of Cx32 in the cells decreased with time in culture. However, when the cells were treated with 2% DMSO from day 4, the immunoreactive spots reappeared on the cell membranes from day 6 and both their number and intensity gradually increased. The reappearance of Cx32 was accompanied by increases in both the protein and mRNA of Cx32. Furthermore, the expression of Cx32 was well maintained, together with extensive gap junctional intercellular communication, for more than 4 weeks. In addition, ultrastructurally, many gap junctional structures were observed between the hepatocytes, and the antibodies to Cx32 were shown to bind to those structures. This culture system may be useful for studies of the reconstruction of the gap junctional structure, the intracellular pathways of the proteins, and the regulation of synthesis and processing in differentiated hepatocytes.

scholarly journals Regulation of ATP-citrate lyase in primary cultures of adult rat hepatocytes.

1979 ◽  
Vol 254 (23) ◽  
pp. 12169-12173
Author(s):  
J.T. Spence ◽  
H.C. Pitot ◽  
G. Zalitis
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Atp Citrate Lyase ◽  
Adult Rat ◽  
Citrate Lyase ◽  
Adult Rat Hepatocytes

Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes

1995 ◽  
Vol 108 (8) ◽  
pp. 2771-2780 ◽  
Author(s):  
T. Kojima ◽  
T. Mitaka ◽  
Y. Shibata ◽  
Y. Mochizuki
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Minor Component ◽  
Rat Hepatocytes ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth ◽  
A Minor ◽  
Junction Proteins

In the adult rat hepatocyte, the gap junction proteins consist of a major component, connexin32 (Cx32) and a minor component, connexin26 (Cx26). Although we recently reported our success in inducing and maintaining Cx32 in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor and 2% dimethyl sulfoxide, it was very difficult to induce Cx26 in the primary hepatocytes. In the present study, we found that the addition of 10(−7) M glucagon into the culture medium could dramatically induce Cx26 mRNA and protein. Although the expression of Cx32 mRNA was also influenced by glucagon, the increase of the expression was small. Immunocytochemically, Cx26-positive spots were observed between most adjacent cells and were co-localized with the Cx32-positive spots. We also examined whether 0.5 mM dibutyl cyclic AMP could induce expression of Cx26 in the cells. The effect of dexamethasone on the expression of Cx26 mRNA compared to that of Cx32 mRNA was examined. For the induction and maintenance of Cx26 mRNA, more than 10(−7) M dexamethasone was necessary in this culture. These results suggest that expression of Cx26 in hepatocytes may be regulated by the concentrations of glucagon and glucocorticoid hormones.

Thymidine uptake in primary cultures of adult rat hepatocytes

1986 ◽  
Vol 14 (1) ◽  
pp. 101-102 ◽  
Author(s):  
ANNA ZAKHAROVA ◽  
HEATHER M. WALLACE
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Thymidine Uptake ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

Induction of cytochrome P-450 by dimethyl sulfoxide in primary cultures of adult rat hepatocytes

1992 ◽  
Vol 61 (2-3) ◽  
pp. 275-281 ◽  
Author(s):  
Tae Cheon Jeong ◽  
Hye Gwang Jeong ◽  
Yang Kyu-Hwan
Keyword(s):  
Dimethyl Sulfoxide ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Cytochrome P 450

scholarly journals Expression of retrovirally transduced genes in primary cultures of adult rat hepatocytes.

1987 ◽  
Vol 84 (10) ◽  
pp. 3344-3348 ◽  
Author(s):  
J. A. Wolff ◽  
J. K. Yee ◽  
H. F. Skelly ◽  
J. C. Moores ◽  
J. G. Respess ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes
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