scholarly journals Effects of Melatonin on Proliferation, Oxidative Stress and Cx32 Gap Junction Protein Expression in Primary Cultures of Adult Rat Hepatocytes.

1997 ◽  
Vol 22 (3) ◽  
pp. 347-356 ◽  
Author(s):  
Takashi Kojima ◽  
Chihiro Mochizuki ◽  
Toshihiro Mitaka ◽  
Yohichi Mochizuki
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Gap Junction ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Junction Protein ◽  
Adult Rat Hepatocytes ◽  
Gap Junction Protein

scholarly journals Regulation of ATP-citrate lyase in primary cultures of adult rat hepatocytes.

1979 ◽  
Vol 254 (23) ◽  
pp. 12169-12173
Author(s):  
J.T. Spence ◽  
H.C. Pitot ◽  
G. Zalitis
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Atp Citrate Lyase ◽  
Adult Rat ◽  
Citrate Lyase ◽  
Adult Rat Hepatocytes

scholarly journals Connexin 43 Gap Junction Protein Expression during Follicular Development in the Porcine Ovary1

1998 ◽  
Vol 58 (2) ◽  
pp. 583-590 ◽  
Author(s):  
Judy A. Lenhart ◽  
Bruce R. Downey ◽  
Carol A. Bagnell
Keyword(s):  
Protein Expression ◽  
Gap Junction ◽  
Connexin 43 ◽  
Follicular Development ◽  
Junction Protein ◽  
Gap Junction Protein

Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes

1995 ◽  
Vol 108 (8) ◽  
pp. 2771-2780 ◽  
Author(s):  
T. Kojima ◽  
T. Mitaka ◽  
Y. Shibata ◽  
Y. Mochizuki
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Minor Component ◽  
Rat Hepatocytes ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth ◽  
A Minor ◽  
Junction Proteins

In the adult rat hepatocyte, the gap junction proteins consist of a major component, connexin32 (Cx32) and a minor component, connexin26 (Cx26). Although we recently reported our success in inducing and maintaining Cx32 in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor and 2% dimethyl sulfoxide, it was very difficult to induce Cx26 in the primary hepatocytes. In the present study, we found that the addition of 10(−7) M glucagon into the culture medium could dramatically induce Cx26 mRNA and protein. Although the expression of Cx32 mRNA was also influenced by glucagon, the increase of the expression was small. Immunocytochemically, Cx26-positive spots were observed between most adjacent cells and were co-localized with the Cx32-positive spots. We also examined whether 0.5 mM dibutyl cyclic AMP could induce expression of Cx26 in the cells. The effect of dexamethasone on the expression of Cx26 mRNA compared to that of Cx32 mRNA was examined. For the induction and maintenance of Cx26 mRNA, more than 10(−7) M dexamethasone was necessary in this culture. These results suggest that expression of Cx26 in hepatocytes may be regulated by the concentrations of glucagon and glucocorticoid hormones.

Thymidine uptake in primary cultures of adult rat hepatocytes

1986 ◽  
Vol 14 (1) ◽  
pp. 101-102 ◽  
Author(s):  
ANNA ZAKHAROVA ◽  
HEATHER M. WALLACE
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Thymidine Uptake ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

Induction of cytochrome P-450 by dimethyl sulfoxide in primary cultures of adult rat hepatocytes

1992 ◽  
Vol 61 (2-3) ◽  
pp. 275-281 ◽  
Author(s):  
Tae Cheon Jeong ◽  
Hye Gwang Jeong ◽  
Yang Kyu-Hwan
Keyword(s):  
Dimethyl Sulfoxide ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Cytochrome P 450

Enhancement of liver cell gap junction protein expression by glucocorticoids

1994 ◽  
Vol 15 (9) ◽  
pp. 1807-1813 ◽  
Author(s):  
Ping Ren ◽  
Adriaan W. de Feijter ◽  
David L. Paul ◽  
Randall J. Ruch
Keyword(s):  
Protein Expression ◽  
Gap Junction ◽  
Liver Cell ◽  
Junction Protein ◽  
Gap Junction Protein
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