scholarly journals Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies)

2017 ◽  
Vol 37 (12) ◽  
pp. 1752-1766 ◽  
Author(s):  
Izabela Dobrowolska ◽  
Edward Businge ◽  
Ilka N Abreu ◽  
Thomas Moritz ◽  
Ulrika Egertsdotter
Keyword(s):  
Picea Abies ◽  
Somatic Embryo ◽  
Norway Spruce ◽  
Transcriptome Profiling ◽  
Embryo Germination ◽  
Somatic Embryo Germination

Effects of Polyethylene Glycol on Development of Grape (Vitis vinifera L. `Thompson Seedless') Somatic Embryos

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 557c-557
Author(s):  
Fred K. Westphal ◽  
Michael E. Compton
Keyword(s):  
Polyethylene Glycol ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
Vitis Vinifera L ◽  
Zygotic Embryos ◽  
Embryo Germination ◽  
Thompson Seedless ◽  
Germination Medium ◽  
Peg Treatment ◽  
Somatic Embryo Germination

Torpedo-stage somatic embryos were selected from actively growing cultures and trasferred to embryo maintenance medium [MS with (per liter) 412.5 mg NH4NO3, 475 mg KNO3, 1 g myo-inositol, 90 g sucrose, 2 g activated charcoal, and 7 g TC agar] supplemented with either 0%, 2.5%, 5%, 7.5%, or 10% polyethylene glycol (PEG) for 4, 8, or 12 weeks. Embryos placed on treatment media were transferred directly to grape somatic embryo germination medium [MS with (per liter) 1 g myo-inositol, 30 g sucrose, 1 M benzyladenine, and 7 g TC agar] once their PEG treatment was terminated. The number of embryos that germinated was recorded 4 weeks after transfer to somatic embryo germination medium. The number of germinated embryos that differentiated into plants was recorded at 8 weeks. There was no difference in germination rates and embryo differentiation among embryos incubated on medium with or without PEG for 4 weeks. A difference in embryo growth rate was observed after 8 weeks on medium with PEG. Embryo grew fastest on media containing 5% or 7.5% PEG. In addition, embryos grown on medium with 5% or 7.5% PEG were morphologically similar to zygotic embryos.

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