Function and Distribution of a Lantipeptide in Strawberry Fusarium Wilt Disease–Suppressive Soils

Streptomyces griseus S4-7 is representative of strains responsible for the specific soil suppressiveness of Fusarium wilt of strawberry caused by Fusarium oxysporum f. sp. fragariae. Members of the genus Streptomyces secrete diverse secondary metabolites including lantipeptides, heat-stable lanthionine-containing compounds that can exhibit antibiotic activity. In this study, a class II lantipeptide provisionally named grisin, of previously unknown biological function, was shown to inhibit F. oxysporum. The inhibitory activity of grisin distinguishes it from other class II lantipeptides from Streptomyces spp. Results of quantitative reverse transcription-polymerase chain reaction with lanM-specific primers showed that the density of grisin-producing Streptomyces spp. in the rhizosphere of strawberry was positively correlated with the number of years of monoculture and a minimum of seven years was required for development of specific soil suppressiveness to Fusarium wilt disease. We suggest that lanM can be used as a diagnostic marker of whether a soil is conducive or suppressive to the disease.

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Genome-wide screening antifungal genes in Streptomyces griseus S4-7, a Fusarium wilt disease suppressive microbial agent

FEMS Microbiology Letters ◽

10.1093/femsle/fnz133 ◽

2019 ◽

Vol 366 (12) ◽

Cited By ~ 1

Author(s):

Sung Won Hong ◽

Da-Ran Kim ◽

Youn Sang Kwon ◽

Youn-Sig Kwak

Keyword(s):

Antifungal Activity ◽

Secondary Metabolites ◽

Fusarium Wilt ◽

Streptomyces Griseus ◽

Wilt Disease ◽

Inverse Pcr ◽

Genus Streptomyces ◽

Fusarium Wilt Disease ◽

Genome Wide ◽

A Genome

ABSTRACT Streptomyces is a widely studied bacterial genus, particularly with regard to secondary metabolites and antibiotics production. Streptomyces griseus S4–7 was isolated from a strawberry Fusarium wilt disease suppressive soil, and its biological control ability has been well established. However, the antifungal mechanism of strain S4–7 is not yet fully understood at the molecular and biochemical level. Therefore, in this study we created a random mutant library for strain S4–7 with the Tn5 transposon element to investigate antifungal traits on a genome-wide scale. In total 4646 individual mutant strains were created and 13 mutants were selected based on loss of antifungal activity. The knockout genes were identified as electron transfer oxidoreductase (eto),sigma factor-70(sig70) and nrps by Inverse PCR (I-PCR). eto regulates the geranylgeranyl reductase gene, which is involved in terpenoid-quinone biosynthesis, an important factor in cell fitness. In the △eto strain, expression of wbl, a master regulator of the production of secondary metabolites, was significantly reduced. sig70 is responsible for the cell differentiation sensing mechanism in genus Streptomyces. △nrps showed decreased production of hybrid peptide-polyketide siderophores. These results suggest that S. griseus S4–7 may have various antifungal mechanisms, and each mechanism is essential to maximal antifungal activity.

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Occurrence of Soil Suppressiveness to Fusarium Wilt Disease of Broad Bean in Ismailia Governorate

Journal of Phytopathology ◽

10.1111/j.1439-0434.1998.tb04777.x ◽

1998 ◽

Vol 146 (8-9) ◽

pp. 431-435 ◽

Cited By ~ 1

Author(s):

O. A. Abdul Wahid ◽

M. E. Ibrahim ◽

M. A. Omar

Keyword(s):

Fusarium Wilt ◽

Broad Bean ◽

Wilt Disease ◽

Soil Suppressiveness ◽

Fusarium Wilt Disease

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ENHANCING SOIL SUPPRESSIVENESS USING FORMULATED GLIOCLADIUM TO CONTROL BANANA FUSARIUM WILT DISEASE

Acta Horticulturae ◽

10.17660/actahortic.2013.975.25 ◽

2013 ◽

pp. 231-237 ◽

Cited By ~ 1

Author(s):

C. Hermanto ◽

Eliza ◽

D. Emilda

Keyword(s):

Fusarium Wilt ◽

Wilt Disease ◽

Soil Suppressiveness ◽

Fusarium Wilt Disease

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INCREASING SOIL SUPPRESSIVITY TO FUSARIUM WILT OF BANANA THROUGH BANANA INTERCROPPING WITH Allium spp. (PENINGKATAN SUPRESIFITAS TANAH TERHADAP PENYAKIT LAYU FUSARIUM PISANG MELALUI PERTANAMAN TUMPANGSARI ANTARA PISANG DAN Allium spp.)

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.16344 ◽

2016 ◽

Vol 19 (1) ◽

pp. 33

Author(s):

Arif Wibowo ◽

Aulia Rahman Alboneh ◽

Medina Uli Alba Somala ◽

Siti Subandiyah ◽

Tony Pattison ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Soil Analysis ◽

Wilt Disease ◽

Plant Diseases ◽

Fluorescein Diacetate ◽

Control Treatment ◽

Soil Suppressiveness ◽

Horticultural Crops ◽

Fusarium Wilt Disease

Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense (Foc), is one of the most destructive diseases of banana and has spread in many plantation areas in Indonesia. Until today, the effective ways to control banana fusarium wiltdisease have not yet been found. Some studies indicated thatAllium spp. could be used to suppress plant diseases caused by Fusarium. Allium spp. are important horticultural crops which are generally cultivated in some areas in Indonesia. This research was conducted to determine the effect of several species ofAllium spp. intercropped with banana to improve soil suppressiveness against banana fusarium wilt disease. The results showed that up to 12 months after planting, from 3 species ofAllium spp. (A. tuberosum/ Chinese leek, A. fistulosum/ bunching onion, and A. cepa var. aggregatum/ shallot) intercropped with banana Ambon Kuning (AAA) cultivar, Chinese leek and shallot were able to suppress the incidence of fusarium wilt disease of banana by 46 and 33% respectively. Soil analysis on the rhizosphere of banana intercropped with Chinese leek and shallot had lower population of total Fusarium compared to the other treatments. Analysis of fluorescein diacetate (3’.6’-diacetylfluoerescein) or FDA also showed that total microbial activity in the rhizosphere of banana intercropped withAllium spp. was also lower compared to control treatment (without intercropping). The observation of the effect ofAllium spp. extracts on Foc showed that Allium spp. extracts were able to suppress the development of the colony and spore germination of Foc in vitro.Keywords: Allium spp., banana, fusarium wilt suppression, intercroppingLayu Fusarium, yang disebabkan oleh Fusarium oxysporum f.sp. cubense (Foc), merupakan salah satu penyakit tanaman pisang yang paling merusak dan telah tersebar di berbagai daerah di Indonesia. Sampai saat ini cara yangefektif untuk mengendalikan penyakit layu fusarium pisang belum ditemukan. Beberapa penelitian menunjukkan bahwa Allium spp. dapat dipergunakan untuk menekan penyakit tumbuhan yang disebabkan oleh Fusarium. Allium spp.adalah tanaman hortikultura penting yang banyak dibudidayakan di Indonesia. Penelitian ini dilakukan untuk mengetahui pengaruh beberapa spesiesAllium spp. yang ditumpangsarikan dengan tanaman pisang untuk meningkatkansupresifitas tanah dalam menekan penyakit layu fusarium pisang. Hasil pengamatan menunjukkan bahwa sampai 12 bulan setelah tanam, dari 3 spesiesAllium spp. (A. tuberosum/kucai, A. fistulosum/loncang, dan A. cepa var. aggregatum/bawang merah) yang ditumpangsarikan dengan pisang kultivar Ambon Kuning (AAA), kucai dan bawang merah mampu menekan insidensi penyakit layu fusarium pisang berturut-turut sebesar 46% dan 33%. Analisis tanah rhizosfer pisang yang ditumpang sari dengan kucai dan bawang merah menunjukkan populasi total Fusarium yang lebih rendah daripada perlakuan lainnya. Analisis fluorescein diacetate (3’.6’-diacetylfluoerescein) atau FDA juga menunjukkan bahwa aktivitas total mikrobia pada rhizosfer pisang yang ditumpang sari dengan Allium spp. lebih rendah jika dibandingkan dengan perlakuan kontrol (tanpa tumpang sari). Pengamatan pengaruh ekstrak Allium spp. terhadap Foc menunjukkan bahwa ekstrak Allium spp. mampu menekan perkembangan koloni dan perkecambahan spora Foc secara in vitro.Kata kunci: Allium spp., penekanan layu fusarium, pisang, tumpang sari

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