scholarly journals cDNA-AFLP Display for the Isolation of Peronospora parasitica Genes Expressed During Infection in Arabidopsis thaliana

2000 ◽  
Vol 13 (8) ◽  
pp. 895-898 ◽  
Author(s):  
Erik A. van der Biezen ◽  
Hedyani Juwana ◽  
Jane E. Parker ◽  
Jonathan D. G. Jones

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.

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