A Comparison of Peronospora parasitica (Downy Mildew) Isolates from Arabidopsis thaliana and Brassica oleracea Using Amplified Fragment Length Polymorphism and Internal Transcribed Spacer 1 Sequence Analyses

2000 ◽  
Vol 30 (2) ◽  
pp. 95-103 ◽  
Author(s):  
Anne P. Rehmany ◽  
James R. Lynn ◽  
Mahmut Tör ◽  
Eric B. Holub ◽  
Jim L. Beynon
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Oleracea ◽  
Downy Mildew ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Internal Transcribed Spacer ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Peronospora Parasitica ◽  
Sequence Analyses

scholarly journals cDNA-AFLP Display for the Isolation of Peronospora parasitica Genes Expressed During Infection in Arabidopsis thaliana

2000 ◽  
Vol 13 (8) ◽  
pp. 895-898 ◽  
Author(s):  
Erik A. van der Biezen ◽  
Hedyani Juwana ◽  
Jane E. Parker ◽  
Jonathan D. G. Jones
Keyword(s):  
Arabidopsis Thaliana ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Peronospora Parasitica ◽  
Differential Gene

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.

Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea

Genome ◽  
2005 ◽  
Vol 48 (5) ◽  
pp. 848-854 ◽  
Author(s):  
Xiaowu Wang ◽  
Ping Lou ◽  
Guusje Bonnema ◽  
Baojun Yang ◽  
Hangjun He ◽  
...  
Keyword(s):  
Male Sterility ◽  
Brassica Oleracea ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Dominant Male ◽  
Male Sterile ◽  
Sterility Gene ◽  
Male Sterility Gene

The dominant male sterility gene Ms-cd1 (c, cabbage; d, dominant) was identified as a spontaneous mutation in the spring cabbage line 79-399-3. The Ms-cd1 gene is successfully applied in hybrid seed production of several Brassica oleracea cultivars in China. Amplified fragment length polymorphism (AFLP) technology was used to identify markers linked to the Ms-cd1 gene in bulks of male-sterile and male-fertile individuals of a segregating BC3 population and in a near-isogenic population of 25 male-sterile plants. Twelve markers within a 20-cM interval proximal to the Ms-cd1 gene were identified, 5 of which can be used to select homozygous male-sterile Ms-cd1/ Ms-cd1 plants. Three AFLP markers and 3 sequence characterized amplified region markers that were linked to MS-cd1 mapped onto linkage group O9, corresponding to chromosome 3 of B. oleracea. This region corresponds to the top of chromosome 5 in Arabidopsis thaliana.Key words: Brassica oleracea, dominant male-sterility gene, bulk segregant analysis, amplified fragment length polymorphism (AFLP), genetic mapping.

scholarly journals Amplified Fragment Length Polymorphism Analysis and Internal Transcribed Spacer and coxII Sequences Reveal a Species Boundary Within Pythium irregulare

2005 ◽  
Vol 95 (12) ◽  
pp. 1489-1498 ◽  
Author(s):  
Carla D. Garzón ◽  
David M. Geiser ◽  
Gary W. Moorman
Keyword(s):  
Cryptic Species ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Internal Transcribed Spacer ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Its Region ◽  
The United States ◽  
Sensu Stricto ◽  
Pythium Irregulare

Pythium irregulare is a plant-pathogenic oomycete that causes significant damage to a variety of crops, including ornamentals and vegetables. Morphological as well as molecular studies have reported high levels of genetic diversity within P. irregulare sensu lato which has raised the question as to whether it is a single species or is actually a complex of morphologically similar (cryptic) species. In this study, we used amplified fragment length polymorphism (AFLP) fingerprinting and DNA sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal genes (ITS region) and a portion of the mitochondrial cytochrome oxidase II gene and the spacer region between coxI and coxII to characterize 68 isolates of P. irregulare from the United States. The ITS sequence of a P. irregulare neotype at the CBS collection as well as ITS and coxII sequences for P. irregulare, P. spinosum, and P. sylvaticum from previous studies were included in our analysis. Cluster analysis identified a 19-isolate group (IR-II) that separated itself from the rest of the sample (IR-I). Population structure and sequence analyses supported the distinction of IR-I and IR-II and identified IR-II as P. irregulare sensu stricto. IR-I was designated Pythium sp. clade IR-I. Two insertion/deletion mutations and nine nucleotide substitutions in the ITS region and three in the sequence of coxII and the adjacent spacer region separated the two species. Additionally, they differed significantly (P > 0.01) in the frequency of 182 (77%) AFLP alleles. Gene flow results suggested that P. irregulare sensu stricto and Pythium sp. clade IR-I are cryptic species capable of exchanging favorable alleles (Nm = 0.72).

scholarly journals The Origin of Cultivated Citrus as Inferred from Internal Transcribed Spacer and Chloroplast DNA Sequence and Amplified Fragment Length Polymorphism Fingerprints

2010 ◽  
Vol 135 (4) ◽  
pp. 341-350 ◽  
Author(s):  
Xiaomeng Li ◽  
Rangjin Xie ◽  
Zhenhua Lu ◽  
Zhiqin Zhou
Keyword(s):  
Chloroplast Dna ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Fragment Length Polymorphism ◽  
Sweet Orange ◽  
Amplified Fragment Length Polymorphism ◽  
Sour Orange ◽  
Rough Lemon

Citrus species are among the most important fruit trees in the world and have a long cultivation history. However, until now, the exact genetic origins of cultivated Citrus such as sweet orange (Citrus sinensis), lemon (C. limon), and grapefruit (C. paradisi) have remained unidentified. In the present study, amplified fragment length polymorphism (AFLP) fingerprints, nuclear internal transcribed spacer (ITS), and three plastid DNA regions (psbH – petB, trnL – trnF, and trnS - trnG) of 30 accessions of the cultivated citrus and their putative wild ancestors were analyzed in an attempt to identify their paternal and maternal origins. Molecular phylogenetic trees were constructed based on the AFLP data, and chloroplast DNA and ITS sequences using the genus Poncirus as the outgroup. Our results indicated that bergamot (C. aurantifolia) and lemon were derived from citron (C. medica) and sour orange (C. aurantium), and grapefruit was a hybrid that originated from a cross between pummelo (C. grandis) and sweet orange. Rough lemon (C. limon) was probable as a parent of rangpur lime (C. limonia) and guangxi local lemon (C. limonia). Our data also demonstrated that sweet orange and sour orange were hybrids of mandarin (C. reticulata) and pummelo, while rough lemon was a cross between citron and mandarin. For mexican lime (C. aurantifolia), our molecular data confirmed a species of Papeda to be the female parent and C. medica as the male. These findings provide new information for future study on the taxonomy, evolution, and genetic breeding of Citrus.

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