scholarly journals Gain of Virulence Caused by Insertion of a Pot3 Transposon in a Magnaporthe grisea Avirulence Gene

2001 ◽  
Vol 14 (5) ◽  
pp. 671-674 ◽  
Author(s):  
Seogchan Kang ◽  
Marc Henri Lebrun ◽  
Leonard Farrall ◽  
Barbara Valent
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Magnaporthe Grisea ◽  
Rice Cultivar ◽  
Avirulence Gene ◽  
Disease Resistance Gene ◽  
Rice Cultivars

The avirulence gene AVR-Pita in Magnaporthe grisea prevents the fungus from infecting rice cultivars carrying the disease resistance gene Pi-ta. Insertion of Pot3 transposon into the promoter of AVR-Pita caused the gain of virulence toward Yashiro-mochi, a rice cultivar containing Pi-ta, which demonstrated the ability of Pot3 to move within the M. grisea genome. The appearance of Pot3 in M. grisea seems to predate the diversification of various host-specific forms of the fungus.

scholarly journals Identification of an Avirulence Gene in the Fungus Magnaporthe grisea Corresponding to a Resistance Gene at the Pik Locus

2005 ◽  
Vol 95 (7) ◽  
pp. 768-772 ◽  
Author(s):  
N. Yasuda ◽  
M. T. Noguchi ◽  
Y. Fujita
Keyword(s):  
Resistance Gene ◽  
Genetic Basis ◽  
Magnaporthe Grisea ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Maps ◽  
Avirulence Gene ◽  
Chinese Isolate ◽  
Rice Cultivars ◽  
Almost All

A rice isolate of Magnaporthe grisea collected from China was avirulent on rice cvs. Hattan 3 and 13 other Japanese rice cultivars. The rice cv. Hattan 3 is susceptible to almost all Japanese blast fungus isolates from rice. The genetic basis of avirulence in the Chinese isolate on Japanese rice cultivars was studied using a cross between the Chinese isolate and a laboratory isolate. The segregation of avirulence or virulence was studied in 185 progeny from the cross, and monogenic control was demonstrated for avirulence to the 14 rice cultivars. The resistance gene that corresponds to the avirulence gene (Avr-Hattan 3) is thought to be located at the Pik locus. Resistance and susceptibility in response to the Chinese isolate in F3 lines of a cross of resistant and susceptible rice cultivars were very similar to the Pik tester isolate, Ken54-20. Random amplified polymorphic DNA markers and restriction fragment length polymorphism markers from genetic maps of the fungus were used to construct a partial genetic map of Avr-Hattan 3. We obtained several flanking markers and one co-segregated marker of Avr-Hattan 3 in the 144 mapping population.

The Tomato Cf-9 Disease Resistance Gene Functions in Tobacco and Potato to Confer Responsiveness to the Fungal Avirulence Gene Product Avr9

1998 ◽  
Vol 10 (8) ◽  
pp. 1251 ◽  
Author(s):  
Kim E. Hammond-Kosack ◽  
Saijun Tang ◽  
Kate Harrison ◽  
Jonathan D. G. Jones
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Gene Product ◽  
Avirulence Gene ◽  
Disease Resistance Gene ◽  
Gene Functions

scholarly journals Mutational Analysis of the Arabidopsis RPS2 Disease Resistance Gene and the Corresponding Pseudomonas syringae avrRpt2 Avirulence Gene

2001 ◽  
Vol 14 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Michael J. Axtell ◽  
Timothy W. McNellis ◽  
Mary Beth Mudgett ◽  
Caroline S. Hsu ◽  
Brian J. Staskawicz
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Pseudomonas Syringae ◽  
Resistance Genes ◽  
Mutational Analysis ◽  
Defense Responses ◽  
Avirulence Gene ◽  
Disease Resistance Gene ◽  
In Planta ◽  
Hypersensitive Cell Death

Plants have evolved a large number of disease resistance genes that encode proteins containing conserved structural motifs that function to recognize pathogen signals and to initiate defense responses. The Arabidopsis RPS2 gene encodes a protein representative of the nucleotide-binding site-leucine-rich repeat (NBS-LRR) class of plant resistance proteins. RPS2 specifically recognizes Pseudomonas syringae pv. tomato strains expressing the avrRpt2 gene and initiates defense responses to bacteria carrying avrRpt2, including a hypersensitive cell death response (HR). We present an in planta mutagenesis experiment that resulted in the isolation of a series of rps2 and avrRpt2 alleles that disrupt the RPS2-avrRpt2 gene-for-gene interaction. Seven novel avrRpt2 alleles incapable of eliciting an RPS2-dependent HR all encode proteins with lesions in the C-terminal portion of AvrRpt2 previously shown to be sufficient for RPS2 recognition. Ten novel rps2 alleles were characterized with mutations in the NBS and the LRR. Several of these alleles code for point mutations in motifs that are conserved among NBS-LRR resistance genes, including the third LRR, which suggests the importance of these motifs for resistance gene function.

scholarly journals The Tomato Cf-9 Disease Resistance Gene Functions in Tobacco and Potato to Confer Responsiveness to the Fungal Avirulence Gene Product Avr9

1998 ◽  
Vol 10 (8) ◽  
pp. 1251-1266 ◽  
Author(s):  
Kim E. Hammond-Kosack ◽  
Saijun Tang ◽  
Kate Harrison ◽  
Jonathan D. G. Jones
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Gene Product ◽  
Avirulence Gene ◽  
Disease Resistance Gene ◽  
Gene Functions

scholarly journals Isolation and characterization of disease resistance gene homologues from rice cultivar IR64

Gene ◽  
2000 ◽  
Vol 255 (2) ◽  
pp. 245-255 ◽  
Author(s):  
Lawrence L. Ilag ◽  
Ram C. Yadav ◽  
Ning Huang ◽  
Pamela C. Ronald ◽  
Frederick M. Ausubel
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Cultivar ◽  
Disease Resistance Gene ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document