Rapid detection of red rot disease pathogens (Pythium chondricola/P. porphyrae) in Pyropia yezoensis (Rhodophyta) using PCR-RFLP method

Plant Disease ◽  
2021 ◽  
Author(s):  
Soon Jeong Lee ◽  
Sang-Rae Lee
Keyword(s):  
Large Subunit ◽  
High Specificity ◽  
Pyropia Yezoensis ◽  
Red Rot ◽  
Pythium Species ◽  
Molecular Monitoring ◽  
Red Rot Disease ◽  
Field Samples ◽  
Pcr Rflp ◽  
Rot Disease

Red rot disease is one of the most well-known algal diseases infecting red algae Pyropia species. This disease seriously decreases the quality and quantity of Pyropia aquaculture products in Korea, Japan, and China. Recently we first found that Pythium chondricola (Oomycetes) infecting blades of Pyropia yezoensis. Therefore two Pythium species (P. chondricola/P. porphyrae) have been reported as red rot disease pathogens. In this study, we developed a species-specific molecular marker for discriminating the two red rot disease pathogens. Using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the mitochondrial cytochrome c oxidase subunit 2 (cox2) and nuclear ribosomal RNA large subunit (LSU) regions, these two Pythium species were successfully classified without a sequencing step. This new method showed high specificity and efficiency for detecting red rot disease pathogens at the species level for both of the cultured samples and field samples. Therefore the molecular markers developed in this study are effective for long-term monitoring on the infection and distribution pattern of each Pythium species in Pyropia aquaculture farms. Moreover, the molecular monitoring can provide useful information for predicting infection and preventing mass mortality of Pyropia species by red rot disease.

scholarly journals Transcriptomic Insights into Innate Immunity Responding to Red Rot Disease in Red Alga Pyropia yezoensis

2019 ◽  
Vol 20 (23) ◽  
pp. 5970 ◽  
Author(s):  
Lei Tang ◽  
Liping Qiu ◽  
Cong Liu ◽  
Guoying Du ◽  
Zhaolan Mo ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Pyropia Yezoensis ◽  
Red Rot ◽  
Red Rot Disease ◽  
Necrotrophic Pathogen ◽  
Pythium Porphyrae ◽  
Rot Disease

Pyropia yezoensis, one of the most economically important marine algae, suffers from the biotic stress of the oomycete necrotrophic pathogen Pythium porphyrae. However, little is known about the molecular defensive mechanisms employed by Pyr. yezoensis during the infection process. In the present study, we defined three stages of red rot disease based on histopathological features and photosynthetic physiology. Transcriptomic analysis was carried out at different stages of infection to identify the genes related to the innate immune system in Pyr. yezoensis. In total, 2139 up-regulated genes and 1672 down-regulated genes were identified from all the infected groups. Pathogen receptor genes, including three lectin genes (pattern recognition receptors (PRRs)) and five genes encoding typical plant R protein domains (leucine rich repeat (LRR), nucleotide binding site (NBS), or Toll/interleukin-1 receptor (TIR)), were found to be up-regulated after infection. Several defense mechanisms that were typically regarded as PAMP-triggered immunity (PTI) in plants were induced during the infection. These included defensive and protective enzymes, heat shock proteins, secondary metabolites, cellulase, and protease inhibitors. As a part of the effector-triggered immunity (ETI), the expression of genes related to the ubiquitin-proteasome system (UPS) and hypersensitive cell death response (HR) increased significantly during the infection. The current study suggests that, similar to plants, Pyr. yezoensis possesses a conserved innate immune system that counters the invasion of necrotrophic pathogen Pyt. porphyrae. However, the innate immunity genes of Pyr. yezoensis appear to be more ancient in origin compared to those in higher plants.

scholarly journals Infection and cox2 sequence of Pythium chondricola (Oomycetes) causing red rot disease in Pyropia yezoensis (Rhodophyta) in Korea

ALGAE ◽  
2017 ◽  
Vol 32 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Soon Jeong Lee ◽  
Bo Young Jee ◽  
Maeng-Hyun Son ◽  
Sang-Rae Lee
Keyword(s):  
Pyropia Yezoensis ◽  
Red Rot ◽  
Red Rot Disease ◽  
Rot Disease

Potential of plant extracts in combination with bacterial antagonist treatment as biocontrol agent of red rot of sugarcane

2007 ◽  
Vol 53 (2) ◽  
pp. 196-206 ◽  
Author(s):  
V. Jayakumar ◽  
R. Bhaskaran ◽  
S. Tsushima
Keyword(s):  
Plant Extracts ◽  
Field Experiments ◽  
Curcuma Longa ◽  
Saccharum Officinarum ◽  
Red Rot ◽  
Colletotrichum Falcatum ◽  
Similar Response ◽  
Red Rot Disease ◽  
Leaf Extracts ◽  
Rot Disease

Plant extracts and antifungal microorganisms were tested singly and in combination for biocontrol of sugarcane red rot disease ( Colletotrichum falcatum ) using two sugarcane ( Saccharum officinarum L.) cultivars, CoC671 and CoC92061, in pot and field experiments. Leaf extracts of Abrus precatorius and Bassia latifolia and the rhizome extract of Curcuma longa reduced Colletotrichum falcatum mycelial growth by 80%, 58%, and 57%, respectively. Although sugarcane- planting materials (setts) treated individually with either Pseudomonas fluorescens Md1 or A. precatorius in pot experiments had the lowest incidences of red rot, 20.1% and 24.2%, respectively, none of the plant extracts were effective in the field. In contrast, when the two varieties were tested separately in two field locations, the setts treated with A. precatorius in combination with a spray or soil application of P. fluorescens Md1 had the lowest incidence of red rot in both locations, e.g., 3.1% and 3.4% incidence for CoC92061 in one location, and had a similar response to the chemical control. The results suggest the applicability of plant-based extracts for the suppression of sugarcane red rot disease in the field as an environment-friendly tool in combination with antagonists.

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