scholarly journals Quantitative trait locus mapping for common scab resistance in a tetraploid potato full-sib population

Plant Disease ◽  
2021 ◽  
Author(s):  
Guilherme Da Silva Pereira ◽  
Marcelo Mollinari ◽  
Xinshun Qu ◽  
Christian Thill ◽  
Zhao-Bang Zeng ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Common Scab ◽  
Negative Impact ◽  
Potato Industry ◽  
Scab Resistance ◽  
Bacterial Disease ◽  
Streptomyces Spp ◽  
Single Quantitative Trait Locus ◽  
Trait Locus

Despite the negative impact of common scab (Streptomyces spp.) on the potato industry, little is known about the genetic architecture of resistance to this bacterial disease in the crop. We evaluated a mapping population (~150 full-sibs) derived from a cross between two tetraploid potatoes (‘Atlantic’ × B1829-5) in three environments (MN11, PA11, ME12) under natural common scab pressure. Three measures to common scab reaction, namely percentage of scabby tubers, and disease area and lesion indices, were found to be highly correlated (>0.76). Due to the large environmental effect, heritability values were zero for all three traits in MN11, but moderate to high in PA11 and ME12 (0.44~0.79). We identified a single quantitative trait locus (QTL) for lesion index in PA11, ME12 and joint analyses on linkage group 3, explaining 22~30% of the total variation. The identification of QTL haplotypes and candidate genes contributing to disease resistance can support genomics-assisted breeding approaches in the crop.

scholarly journals Quantitative trait locus mapping for common scab resistance in a tetraploid potato full-sib population

2020 ◽  
Author(s):  
Guilherme da Silva Pereira ◽  
Marcelo Mollinari ◽  
Xinshun Qu ◽  
Christian Thill ◽  
Zhao-Bang Zeng ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Common Scab ◽  
Negative Impact ◽  
Potato Industry ◽  
Scab Resistance ◽  
Bacterial Disease ◽  
Single Quantitative Trait Locus ◽  
Trait Locus ◽  
Highly Correlated

AbstractDespite the negative impact of common scab (Streptomyces spp.) to the potato industry, little is known about the genetic architecture of resistance to this bacterial disease in the crop. We evaluated a mapping population (~150 full-sibs) derived from a cross between two tetraploid potatoes (‘Atlantic’ × B1829-5) in three environments (MN11, PA11, ME12) under natural common scab pressure. Three measures to common scab reaction were assessed, namely percentage of scabby tubers, and disease area and lesion indices, which were highly correlated (>0.76). Due to large environmental effect, heritability values were zero for all three traits in MN11, but moderate to high in PA11 and ME12 (0.44~0.79). We identified a single quantitative trait locus (QTL) for lesion index in PA11, ME12 and joint analyses on linkage group 3, explaining 22~30% of the total variation. The identification of QTL haplotypes and candidate genes contributing to disease resistance can support genomics-assisted breeding approaches.

Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 719-727 ◽  
Author(s):  
Wenchun Zhou ◽  
Frederic L Kolb ◽  
Guihua Bai ◽  
Gregory Shaner ◽  
Leslie L Domier
Keyword(s):  
Quantitative Trait Locus ◽  
Microsatellite Markers ◽  
Quantitative Trait ◽  
Chromosome Region ◽  
Recombinant Inbred Lines ◽  
Scab Resistance ◽  
Aflp Analysis ◽  
Resistance Qtl ◽  
Trait Locus ◽  
Major Qtl

Three chromosomal regions associated with scab resistance were detected in a common cultivar, Ning7840, by microsatellite and AFLP analysis. Six microsatellites on chromosome 3BS, Xgwm389, Xgwm533, Xbarc147, Xgwm493, Xbarc102, and Xbarc131, were integrated into an amplified fragment length polymorphism (AFLP) linkage group containing a major quantitative trait locus (QTL) for scab resistance in a mapping population of 133 recombinant inbred lines (RILs) derived from 'Ning7840' × 'Clark'. Based on single-factor analysis of variance of scab infection data from four experiments, Xgwm533 and Xbarc147 were the two microsatellite markers most tightly associated with the major scab resistance QTL. Interval analysis based on the integrated map of AFLP and microsatellite markers showed that the major QTL was located in a chromosome region about 8 cM in length around Xgwm533 and Xbarc147. Based on mapping of six microsatellite markers on eight 3BS deletion lines, the major QTL was located distal to breakage point 3BS-8. In total, 18 microsatellites were physically located on different subarm regions on 3BS. Two microsatellites, Xgwm120 and Xgwm614, were significantly associated with QTL for scab resistance on chromosome 2BL and 2AS, respectively. The resistance alleles on 3BS, 2BL, and 2AS were all derived from 'Ning7840'. Significant interaction between the major QTL on 3BS and the QTL on 2BL was detected based on microsatellite markers linked to them. Using these microsatellite markers would facilitate marker-assisted selection to improve scab resistance in wheat.Key words: Fusarium head blight, quantitative trait locus, physical mapping, Triticum aestivum L.

scholarly journals Amplified Fragment Length Polymorphism Markers Linked to a Major Quantitative Trait Locus Controlling Scab Resistance in Wheat

1999 ◽  
Vol 89 (4) ◽  
pp. 343-348 ◽  
Author(s):  
Guihua Bai ◽  
Frederic L. Kolb ◽  
Gregory Shaner ◽  
Leslie L. Domier
Keyword(s):  
Quantitative Trait Locus ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Major Quantitative Trait Locus ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Scab Resistance ◽  
Trait Locus

Scab is a destructive disease of wheat. To accelerate development of scab-resistant wheat cultivars, molecular markers linked to scab resistance genes have been identified by using recombinant inbred lines (RILs) derived by single-seed descent from a cross between the resistant wheat cultivar Ning 7840 (resistant to spread of scab within the spike) and the susceptible cultivar Clark. In the greenhouse, F5, F6, F7, and F10 families were evaluated for resistance to spread of scab within a spike by injecting about 1,000 conidiospores of Fusarium graminearum into a central spikelet. Inoculated plants were kept in moist chambers for 3 days to promote initial infection and then transferred to greenhouse benches. Scab symptoms were evaluated four times (3, 9, 15, and 21 days after inoculation). The frequency distribution of scab severity indicated that resistance to spread of scab within a spike was controlled by a few major genes. DNA was isolated from both parents and F9 plants of the 133 RILs. A total of 300 combinations of amplified fragment length polymorphism (AFLP) primers were screened for polymorphisms using bulked segregant analysis. Twenty pairs of primers revealed at least one polymorphic band between the two contrasting bulks. The segregation of each of these bands was evaluated in the 133 RILs. Eleven AFLP markers showed significant association with scab resistance, and an individual marker explained up to 53% of the total variation (R2). The markers with high R2 values mapped to a single linkage group. By interval analysis, one major quantitative trait locus for scab resistance explaining up to 60% of the genetic variation for scab resistance was identified. Some of the AFLP markers may be useful in marker-assisted breeding to improve resistance to scab in wheat.

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