scholarly journals Effects of Verticillium dahliae Infection of Cotton Plants (Gossypium hirsutum) on Potassium Levels in Leaf Petioles

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1089-1092 ◽  
Author(s):  
J. E. DeVay ◽  
B. L. Weir ◽  
R. J. Wakeman ◽  
J. J. Stapleton
Keyword(s):  
Gossypium Hirsutum ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Potassium Deficiency ◽  
Potassium Content ◽  
Sterile Water ◽  
Deficiency Symptoms ◽  
Cotton Plants ◽  
Gradual Development ◽  
Agar Media

Two isolates of Verticillium dahliae, a black microsclerotial isolate and an isolate from potassium deficient cotton plants that forms white colonies on agar media, were examined for their effects on the potassium content of cotton plants. The potassium content of petioles from fully expanded leaves collected at random from branches 6 to 7 nodes below the terminal node were monitored during July and August in 1993 to 1995. Potassium contents of petioles from plants inoculated with V. dahliae did not differ significantly from plants injected with sterile water until the plants were nearing peak boll load. Both isolates caused a gradual development of potassium deficiency symptoms in leaves of inoculated plants and a decrease in petiole potassium, often accompanied by chlorosis and necrosis typical of Verticillium wilt. These results suggest that infection of cotton plants by V. dahliae causes an impairment in the uptake and translocation of potassium that is often associated with the development of potassium deficiency symptoms in leaves of plants with large boll loads.

scholarly journals GhKWL1 Upregulates GhERF105 but Its Function Is Impaired by Binding with VdISC1, a Pathogenic Effector of Verticillium dahliae

2021 ◽  
Vol 22 (14) ◽  
pp. 7328
Author(s):  
Yang Chen ◽  
Mi Zhang ◽  
Lei Wang ◽  
Xiaohan Yu ◽  
Xianbi Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Gossypium Hirsutum ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Upland Cotton ◽  
Effector Protein ◽  
Positive Regulator ◽  
Its Gene

Verticillium wilt, caused by Verticillium dahliae, is a devastating disease for many important crops, including cotton. Kiwellins (KWLs), a group of cysteine-rich proteins synthesized in many plants, have been shown to be involved in response to various phytopathogens. To evaluate genes for their function in resistance to Verticillium wilt, we investigated KWL homologs in cotton. Thirty-five KWL genes (GhKWLs) were identified from the genome of upland cotton (Gossypium hirsutum). Among them, GhKWL1 was shown to be localized in nucleus and cytosol, and its gene expression is induced by the infection of V. dahliae. We revealed that GhKWL1 was a positive regulator of GhERF105. Silencing of GhKWL1 resulted in a decrease, whereas overexpression led to an increase in resistance of transgenic plants to Verticillium wilt. Interestingly, through binding to GhKWL1, the pathogenic effector protein VdISC1 produced by V. dahliae could impair the defense response mediated by GhKWL1. Therefore, our study suggests there is a GhKWL1-mediated defense response in cotton, which can be hijacked by V. dahliae through the interaction of VdISC1 with GhKWL1.

Histochemistry and identification of disease-induced terpenoid aldehydes in Verticillium-wilt-resistant and -susceptible cottons

1976 ◽  
Vol 54 (18) ◽  
pp. 2095-2099 ◽  
Author(s):  
M. E. Mace ◽  
A. A. Bell ◽  
C. H. Beckman
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Cellular Localization ◽  
Fungal Colonization ◽  
Parenchyma Cells ◽  
Cotton Plants ◽  
Terpenoid Aldehydes

Hemigossypol (HG) and 6-methoxyhemigossypol (MHG) were the major terpenoid aldehydes isolated from stem stele tissue of 6-week-old verticillium-wilt-susceptible Rowden and verticillium-wilt-resistant Seabrook Sea Island 12B2 (SBSI) cotton plants infected for 14 days with Verticillium dahliae. HG constituted 90 and 45 mol % and MHG 6 and 41 mol % of the induced terpenoid in the steles of Rowden and SBSI, respectively. No terpenoids were detected in extracts of noninfected steles.The cellular localization of terpenoids in infected and noninfected stems was studied histochemically with a SbCl3 reagent. Terpenoids first occurred in both SBSI and Rowden in scattered, usually solitary, paratracheal parenchyma cells appressed to infected xylem vessels. After extensive fungal colonization, diffusion of induced terpenoids obscured the initial, discrete sites of localization.

scholarly journals Transcriptome analysis reveals the defense mechanism of cotton against Verticillium dahliae in the presence of the biocontrol fungus Chaetomium globosum CEF-082

2019 ◽  
Author(s):  
YUN ZHANG ◽  
Na Yang ◽  
Lihong Zhao ◽  
Heqin Zhu ◽  
Canming Tang(New Corresponding Author)
Keyword(s):  
Molecular Mechanism ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Defense Mechanism ◽  
Substantial Reduction ◽  
Metabolic Process ◽  
Chaetomium Globosum ◽  
Post Inoculation ◽  
Cotton Plants

Abstract Background: Verticillium wilt of cotton is a serious soil-borne disease that causes a substantial reduction in cotton yields. A previous study showed that the endophytic fungus Chaetomium globosum CEF-082 could control Verticillium wilt of cotton, and induce a defense response in cotton plants. However, the comprehensive molecular mechanism governing this response is not yet clear. Results: To study the signalling mechanism induced by CEF-082, the transcriptome of cotton seedlings pretreated with CEF-082 was sequenced. The results revealed 5638 DEGs at 24 h post inoculation with CEF-082, and 2921 and 2153 DEGs at 12 and 48 h post inoculation with Verticillium dahliae, respectively. At 24 h post inoculation with CEF-082, KEGG enrichment analysis indicated that the DEGs were enriched mainly in the plant-pathogen interaction, MAPK signalling pathway-plant, flavonoid biosynthesis, and phenylpropanoid biosynthesis pathways. There were 1209 DEGs specifically induced only in cotton plants inoculated with V. dahliae in the presence of the biocontrol fungus CEF-082, and not when cotton plants were only inoculated with V. dahliae. GO analysis revealed that these DEGs were enriched mainly in the following terms: ROS metabolic process, H2O2 metabolic process, defense response, superoxide dismutase activity, and antioxidant activity. Moreover, many genes, such as ERF, CNGC, FLS2, MYB, GST and CML, that regulate crucial points in defense-related pathways were identified and may contribute to V. dahliae resistance in cotton. These results provide a basis for understanding the molecular mechanism by which the biocontrol fungus CEF-082 increases the resistance of cotton to Verticillium wilt. Conclusions: The results of this study showed that CEF-082 could regulate multiple metabolic pathways in cotton. After treatment with V. dahliae, the defense response of cotton plants preinoculated with CEF-082 was strengthened.

scholarly journals Transcriptome analysis reveals the defense mechanism of cotton against Verticillium dahliae in the presence of the biocontrol fungus Chaetomium globosum CEF-082

2019 ◽  
Author(s):  
YUN ZHANG ◽  
Na Yang ◽  
Lihong Zhao ◽  
Heqin Zhu ◽  
Canming Tang
Keyword(s):  
Molecular Mechanism ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Defense Mechanism ◽  
Substantial Reduction ◽  
Metabolic Process ◽  
Chaetomium Globosum ◽  
Post Inoculation ◽  
Cotton Plants

Abstract Background: Verticillium wilt of cotton is a serious soil-borne disease that causes a substantial reduction in cotton yields. A previous study showed that the endophytic fungus Chaetomium globosum CEF-082 could control Verticillium wilt of cotton, and induce a defense response in cotton plants. However, the comprehensive molecular mechanism governing this response is not yet clear. Results: To study the signalling mechanism induced by CEF-082, the transcriptome of cotton seedlings pretreated with CEF-082 was sequenced. The results revealed 5638 DEGs at 24 h post inoculation with CEF-082, and 2921 and 2153 DEGs at 12 and 48 h post inoculation with Verticillium dahliae , respectively. At 24 h post inoculation with CEF-082, KEGG enrichment analysis indicated that the DEGs were enriched mainly in the plant-pathogen interaction, MAPK signalling pathway-plant, flavonoid biosynthesis, and phenylpropanoid biosynthesis pathways. There were 1209 DEGs specifically induced only in cotton plants inoculated with V. dahliae in the presence of the biocontrol fungus CEF-082, and not when cotton plants were only inoculated with V. dahliae . GO analysis revealed that these DEGs were enriched mainly in the following terms: ROS metabolic process, H 2 O 2 metabolic process, defense response, superoxide dismutase activity, and antioxidant activity. Moreover, many genes, such as ERF , CNGC , FLS2 , MYB , GST and CML , that regulate crucial points in defense-related pathways were identified and may contribute to V. dahliae resistance in cotton. These results provide a basis for understanding the molecular mechanism by which the biocontrol fungus CEF-082 increases the resistance of cotton to Verticillium wilt. Conclusions: The results of this study showed that CEF-082 could regulate multiple metabolic pathways in cotton. After treatment with V. dahliae , the defense response of cotton plants preinoculated with CEF-082 was strengthened.

Inheritance of resistance to Verticillium wilt (Verticillium dahliae) in cotton (Gossypium hirsutum L.)

2005 ◽  
Vol 124 (1) ◽  
pp. 102-104 ◽  
Author(s):  
M. Mert ◽  
S. Kurt ◽  
O. Gencer ◽  
Y. Akiscan ◽  
K. Boyaci ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Inheritance Of Resistance ◽  
Gossypium Hirsutum L

scholarly journals Transcriptome analysis reveals the cotton defense mechanism in response to Verticillium dahliae in the presence of the biocontrol fungus Chaetomium globosum CEF-082

2019 ◽  
Author(s):  
Yun Zhang ◽  
Na Yang ◽  
Lihong Zhao ◽  
Heqin Zhu ◽  
Canming Tang
Keyword(s):  
Molecular Mechanism ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Substantial Reduction ◽  
Enrichment Analysis ◽  
Metabolic Process ◽  
Chaetomium Globosum ◽  
Post Inoculation ◽  
Cotton Plants

Abstract Background: Verticillium wilt of cotton is a serious soil-borne disease that causes a substantial reduction in cotton yields. A previous study showed that the endophytic fungus Chaetomium globosum CEF-082 could control Verticillium wilt of cotton, and induce a defense response in cotton plants. However, the comprehensive molecular mechanism governing this response is not clear. Results: To study the signalling mechanism induced by CEF-082, the transcriptome of cotton seedlings pretreated with CEF-082 was sequenced. The results revealed 5638 DEGs at 24 h post inoculation with CEF-082, and 2921 and 2153 DEGs at 12 and 48 h post inoculation with Verticillium dahliae, respectively. At 24 h post inoculation with CEF-082, KEGG enrichment analysis indicated that the DEGs were enriched mainly in the plant-pathogen interaction, MAPK signalling pathway-plant, flavonoid biosynthesis, and phenylpropanoid biosynthesis pathways. There were 1209 DEGs specifically induced only in cotton plants inoculated with V. dahliae in the presence of the biocontrol fungus CEF-082, and not when cotton plants were only inoculated with V. dahliae. GO enrichment analysis revealed that these DEGs were enriched mainly in the following terms: ROS metabolic process, H2O2 metabolic process, defense response, superoxide dismutase activity, and antioxidant activity. Moreover, many genes, such as ERF, CNGC, FLS2, MYB, GST and CML genes, were identified that regulate crucial points in defence-related pathways and that may contribute to V. dahliae resistance in cotton. These results provide a basis for the understanding of the molecular mechanism by which biocontrol fungus CEF-082 increased the resistance of cotton to Verticillium wilt. Conclusions: The results of this study showed that CEF-082 could regulate multiple metabolic pathways in cotton. After treatment with V. dahliae, the defense response of cotton plants preinoculated with CEF-082 was strengthened.

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