Diversity of culturable endophytic fungi vary through time in Manihot esculenta Crantz

Endophytic fungi are a ubiquituos group that colonize all plant species on earth. Studies comparing the location of endophytic fungi within the leaves and the sampling time in Manihot esculenta Crantz (cassava) are limited. In this study, mature leaves of M. esculenta from Panama were collected in order to compare the cultivable diversity of endophytic fungi and to determine their distribution within the leaves. A total of one hundred sixty endophytes belonging to 97 species representing 13 genera and 8 morphospecies determined as mycelia sterilia that containing 63 isolates were isolated. Cladosporium, Nigrospora, Periconia, and mycelia sterilia 1 and 3 were the most predominant isolated endophytes. We detected that endophytes varied across the sampling time, but not amongst locations within leaves. The endophytes composition across sampling and the location of endophytes within leaf was similar, except for Periconia and mycelia sterilia 3 and 7. The data generated in this study contribute to the knowledge on the biodiversity of endophytic fungi in Panama, and establish the bases for future research focused on understanding the function of endophytes in M. esculenta crops.

Filtrates from cultures of endophytic fungi isolated from leaves of Copaifera oblongifolia (Fabaceae) affect germination and seedling development differently

Interactions between endophytic fungi (EFs) and their host plants range from positive to neutral to negative. The results of such interactions can vary depending on the organ of the infected host plant. EFs isolated from the leaves of some species of plants have potential for use as agents to inhibit seed germination and control invasive plants. The objectives of this study were to identify EFs present in the leaves of Copaifera oblongifolia and to evaluate the role of these fungi in seed germination and seedling development. A total of 11 species of EFs were isolated, which were identified using the internal transcribed spacers (ITS) sequence of the nuclear ribosomal DNA. The isolated species of EFs are generalists and probably are transmitted horizontally. Laboratory tests revealed that filtrates of these fungal isolates differently affect seed germination and seedling development of C. oblongifolia. The species Curvularia intermedia, Neofusicoccum parvum, Pseudofusicoccum stromaticum and Phomopsis sp. negatively affected seed germination, with N. parvum standing out for its negative effects, inhibiting seedling germination and survival in 89 and 222%, respectively. In addition, Cochliobolus intermedius negatively affected seedling development. Thus, the combined use of N. parvum and C. intermedius, or products from the metabolism of these microorganisms, in the control of invasive plants deserves attention from future studies.

Putative Anticancer Compounds from Plant-Derived Endophytic Fungi: A Review

Endophytic fungi are microorganisms that exist almost ubiquitously inside the various tissues of living plants where they act as an important reservoir of diverse bioactive compounds. Recently, endophytic fungi have drawn tremendous attention from researchers; their isolation, culture, purification, and characterization have revealed the presence of around 200 important and diverse compounds including anticancer agents, antibiotics, antifungals, antivirals, immunosuppressants, and antimycotics. Many of these anticancer compounds, such as paclitaxel, camptothecin, vinblastine, vincristine, podophyllotoxin, and their derivatives, are currently being used clinically for the treatment of various cancers (e.g., ovarian, breast, prostate, lung cancers, and leukemias). By increasing the yield of specific compounds with genetic engineering and other biotechnologies, endophytic fungi could be a promising, prolific source of anticancer drugs. In the future, compounds derived from endophytic fungi could increase treatment availability and cost effectiveness. This comprehensive review includes the putative anticancer compounds from plant-derived endophytic fungi discovered from 1990 to 2020 with their source endophytic fungi and host plants as well as their antitumor activity against various cell lines.

Production, Optimization, Characterization and Drought Stress Resistance by β-Glucan-Rich Heteropolysaccharide From an Endophytic Fungi Colletotrichum alatae LCS1 Isolated From Clubmoss (Lycopodium clavatum)

Endophytic entities are ubiquitous in nature with all-square bioactivity ranging from therapeutic effects toward animals to growth promoting attributes and stress tolerance activities in case of green plants. In the present study, the club moss Lycopodium clavatum for the first time has been subjected for the isolation of endophytic fungi. An exopolysaccharide (EPS) extracted from Colletotrichum alatae LCS1, an endophytic fungi isolated from L. clavatum Linn., was characterized as a β-glucan heteropolymer (composed of mannose, rhamnose, arabinose, glucose, galactose, and fucose) which plays a pivotal role in obliterating the drought stress in rice seedlings (Oryza sativa) when applied at an amount of 20, 50, and 100 ppm. The fresh weight contents of rice tissue (39%), total chlorophyll (33%), proline (41%), soluble sugar content (26%) along with antioxidant enzymes such as catalase, peroxidase, and super-oxide dismutase increased (in comparison to control of non-EPS treated seedlings) while malondialdehyde content had reduced markedly after 30 days of regular treatment. The drought resistance of rice seedling was observed at peak when applied at 50 ppm dosage. Vital parameters for EPS production like fermentation duration (5 days), medium pH (6), nutrient (carbon (glucose-7 g%/l), nitrogen (yeast extract-0.4 g%/l), and mineral (NaCl-0.10 g%/l) sources, oxygen requirements (O2 vector or liquid alkane-n-hexane, n-heptane, n-hexadecane), and headspace volume (250 ml Erlenmeyer flask- 50 ml medium, 200 ml-headspace volume) were optimized to obtain an enhanced EPS yield of 17.38 g/L−59% higher than the preoptimized one. The present study, for the first time, reported the β-glucan rich heteropolysaccharide from Colletotrichum origin which is unique in structure and potent in its function of drought stress tolerance and could enhance the sustainable yield of rice cultivation in areas facing severe drought stress.

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

Endophytic fungi are recognised for producing secondary metabolites that have an effect on the host, such as promoting growth and enhancing plant resilience to biotic and abiotic stress. The objective of this study was to explore endophytic fungi from Citrus reticulata. The endophytic fungi were isolated from both healthy and infected roots, stems, and twigs of C. reticulata. The materials were sterilised and inoculated on potato dextrose agar (PDA) media. The fungi grown were transferred onto fresh PDA plates and identified based on their morphological characteristic, including colonial features, hyphae type, and reproduction structure. Five endophytic fungi were identified as Colletotrichum sp. AJSH2-1, Cylindrocladium sp. BJSH1-2, Rhizopus sp. RJSH1-1, Mucor sp. RJSH1-2, and Aspergillus sp. BJSH1-1. Further study is required to understand the ability of the identified genera in protecting the host from plant disease as well as promoting growth.