Performance and Mapping of Leaf Rust Resistance Transferred to Wheat from Triticum timopheevii subsp. armeniacum

Host plant resistance is an economical and environmentally sound method of control of leaf rust caused by the fungus Puccinia triticina, which is one of the most serious diseases of wheat (Triticum aestivum) worldwide. Wild relatives of wheat, including the tetraploid T. timopheevii subsp. armeniacum, represent an important source of genes for resistance to leaf rust. The objectives of this study were to (i) evaluate the performance of leaf rust resistance genes previously transferred to wheat from three accessions of T. timopheevii subsp. armeniacum, (ii) determine inheritance and allelic relationship of the new leaf rust resistance genes, and (iii) determine the genetic map location of one of the T. timopheevii subsp. armeniacum-derived genes using microsatellite markers. The leaf rust resistance gene transferred to hexaploid wheat from accession TA 28 of T. timopheevii subsp. armeniacum exhibited slightly different infection types (ITs) to diverse races of leaf rust in inoculated tests of seedlings compared with the gene transferred from TA 870 and TA 874. High ITs were exhibited when seedlings of all the germ plasm lines were inoculated with P. triticina races MBRL and PNMQ. However, low ITs were observed on adult plants of all lines having the T. timopheevii subsp. armeniacum-derived genes for resistance in the field at locations in Kansas and Texas. Analysis of crosses between resistant germ plasm lines showed that accessions TA 870 and TA 874 donated the same gene for resistance to leaf rust and TA 28 donated an independent resistance gene. The gene donated to germ plasm line KS96WGRC36 from TA 870 of T. timopheevii subsp. armeniacum was linked to microsatellite markers Xgwm382 (6.7 cM) and Xgdm87 (9.4 cM) on wheat chromosome arm 2B long. This new leaf rust resistance gene is designated Lr50. It is the first named gene for leaf rust resistance transferred from wild timopheevi wheat and is the only Lr gene located on the long arm of wheat homoeologous group 2 chromosomes.

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Chromosomal Location and Genetic Relationship of Leaf Rust Resistance Genes Rph9 and Rph12 in Barley

Phytopathology ◽

10.1094/phyto.1998.88.1.76 ◽

1998 ◽

Vol 88 (1) ◽

pp. 76-80 ◽

Cited By ~ 36

Author(s):

I. G. Borovkova ◽

Y. Jin ◽

B. J. Steffenson

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Chromosomal Location ◽

Leaf Rust Resistance ◽

Chromosome 7 ◽

Puccinia Hordei ◽

Leaf Rust Resistance Gene ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Barley lines Hor 2596 and Triumph are the sources of leaf rust resistance genes Rph9 and Rph12, respectively. An allelism test was performed with F2 progeny of the cross Triumph/Hor 2596 inoculated with Puccinia hordei. No recombinants were found in a population of 3,858 progeny, indicating Rph9 and Rph12 are alleles. Molecular and morphological markers were used to identify the chromosomal location of these genes in the crosses Bowman/Hor 2596 and Triumph/I91-533-va. A linkage was detected between Rph9 and the flanking sequence-tagged site (STS) markers ABC155 and ABG3 on chromosome 7(5H) at a distance of 20.6 and 20.1 centimorgans (cM), respectively, and to the microsatellite marker dehydrin-9 (HVDHN9) at a distance of 10.2 cM in the Bowman/ Hor 2596 cross. Analysis of isozymes in bulks of the same population showed that Rph9 may be closely linked to the Est9 locus on chromosome 7(5H). The Rph12 locus was linked to the morphological trait locus va (controlling variegated leaf color) on chromosome 7(5H) at a distance of 22.6 cM in the Triumph/I91-533-va cross. Rph12 also was linked with STS marker ABC155 (24.4 cM) and RAPD marker OPA19 (1.5) (17.8 cM). These data indicate that Hor 2596 and Triumph carry a leaf rust resistance gene at the same locus on the long arm of chromosome 7(5H) of barley.

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Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Crop Science ◽

10.1002/csc2.20057 ◽

2020 ◽

Vol 60 (1) ◽

pp. 282-302 ◽

Cited By ~ 1

Author(s):

Matthew J. Martin ◽

Oswaldo Chicaiza ◽

Juan C. Caffarel ◽

Ahmad H. Sallam ◽

Arnis Druka ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Introgression Lines ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

Open Life Sciences ◽

10.1515/biol-2019-0036 ◽

2019 ◽

Vol 14 (1) ◽

pp. 327-334 ◽

Cited By ~ 2

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Alicja Buda ◽

Danuta Kurasiak-Popowska ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Resistance ◽

Wheat Cultivars ◽

Pcr Conditions ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

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Microsatellite markers for the Triticum timopheevi-derived leaf rust resistance gene Lr18 on wheat 5BL chromosome

Breeding Science ◽

10.1270/jsbbs.16148 ◽

2017 ◽

Vol 67 (2) ◽

pp. 129-134 ◽

Cited By ~ 2

Author(s):

Ali Aliakbari Sadeghabad ◽

Ali Dadkhodaie ◽

Bahram Heidari ◽

Hooman Razi ◽

Reza Mostowfizadeh-Ghalamfarsa

Keyword(s):

Microsatellite Markers ◽

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene ◽

Triticum Timopheevi

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Identification and Molecular Characterization of Leaf Rust Resistance Gene Lr14a in Durum Wheat

Plant Disease ◽

10.1094/pdis-92-3-0469 ◽

2008 ◽

Vol 92 (3) ◽

pp. 469-473 ◽

Cited By ~ 43

Author(s):

S. A. Herrera-Foessel ◽

R. P. Singh ◽

J. Huerta-Espino ◽

H. M. William ◽

V. Garcia ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Gene ◽

Ssr Markers ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

Leaf rust, caused by Puccinia triticina, is an important disease of durum wheat (Triticum turgidum subsp. durum) and only a few designated resistance genes are known to occur in this crop. A dominant leaf rust resistance gene in the Chilean durum cv. Llareta INIA was mapped to chromosome arm 7BL through bulked segregant analysis using the amplified fragment length polymorphism (AFLP) technique, and by mapping three polymorphic markers in the common wheat (T. aestivum) International Triticeae Mapping Initiative population. Several simple sequence repeat (SSR) markers, including Xgwm344-7B and Xgwm146-7B, were associated with the leaf rust resistance gene. Resistance response and chromosomal position indicated that this gene is likely to be Lr14a. The SSR markers Xgwm344-7B and Xgwm146-7B and one AFLP marker also differentiated common wheat cv. Thatcher from the near-isogenic line with Lr14a, as well as durum ‘Altar C84’ from durum wheat with Lr14a. This is the first report of the presence of Lr14a in durum wheat, although the gene originally was transferred from emmer wheat ‘Yaroslav’ to common wheat. Lr14a is also present in CIMMYT-derived durum ‘Somateria’ and effective against Mexican and other P. triticina races of durum origin. Lr14a should be deployed in combination with other effective leaf rust resistance genes to prolong its effectiveness in durum wheat.

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