scholarly journals Voltage‐gated K+ (Kv) currents sensitive to stromatoxin‐1 in Guinea pig urinary bladder smooth muscle

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Kiril L. Hristov ◽  
Muyan Chen ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Smooth Muscle ◽  
Voltage Gated ◽  
Urinary Bladder Smooth Muscle

scholarly journals Voltage-gated K+ channels sensitive to stromatoxin-1 regulate myogenic and neurogenic contractions of rat urinary bladder smooth muscle

2010 ◽  
Vol 299 (1) ◽  
pp. R177-R184 ◽  
Author(s):  
Muyan Chen ◽  
Whitney F. Kellett ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Electrical Field Stimulation ◽  
Resting Membrane Potential ◽  
Bladder Smooth Muscle ◽  
Membrane Excitability ◽  
Molecular Fingerprints ◽  
Rat Urinary Bladder ◽  
Voltage Gated ◽  
Urinary Bladder Smooth Muscle

Members of the voltage-gated K+ (KV) channel family are suggested to control the resting membrane potential and the repolarization phase of the action potential in urinary bladder smooth muscle (UBSM). Recent studies report that stromatoxin-1, a peptide isolated from tarantulas, selectively inhibits KV2.1, KV2.2, KV4.2, and KV2.1/9.3 channels. The objective of this study was to investigate whether KV channels sensitive to stromatoxin-1 participate in the regulation of rat UBSM contractility and to identify their molecular fingerprints. Stromatoxin-1 (100 nM) increased the spontaneous phasic contraction amplitude, muscle force, and tone in isolated UBSM strips. However, stromatoxin-1 (100 nM) had no effect on the UBSM contractions induced by depolarizing agents such as KCl (20 mM) or carbachol (1 μM). This indicates that, under conditions of sustained membrane depolarization, the KV channels sensitive to stromatoxin-1 have no further contribution to the membrane excitability and contractility. Stromatoxin-1 (100 nM) increased the amplitude of the electrical field stimulation-induced contractions, suggesting also a role for these channels in neurogenic contractions. RT-PCR experiments on freshly isolated UBSM cells showed mRNA expression of KV2.1, KV2.2, and KV9.3, but not KV4.2 channel subunits. Protein expression of KV2.1 and KV2.2 channels was detected using Western blot and was further confirmed by immunocytochemical detection in freshly isolated UBSM cells. These novel findings indicate that KV2.1 and KV2.2, but not KV4.2, channel subunits are expressed in rat UBSM and play a key role in opposing both myogenic and neurogenic UBSM contractions.

Functional Characterization of Adenosine Receptors and Coupling to ATP-Sensitive K+ Channels in Guinea Pig Urinary Bladder Smooth Muscle

2002 ◽  
Vol 300 (3) ◽  
pp. 910-917 ◽  
Author(s):  
Sujatha M. Gopalakrishnan ◽  
Steven A. Buckner ◽  
Ivan Milicic ◽  
Duncan R. Groebe ◽  
Kristi L. Whiteaker ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Adenosine Receptors ◽  
Functional Characterization ◽  
Bladder Smooth Muscle ◽  
K Channels ◽  
Urinary Bladder Smooth Muscle

scholarly journals Phospholipase C Inhibitors Suppress Spontaneous Mechanical Activity of Guinea Pig Urinary Bladder Smooth Muscle

2003 ◽  
Vol 26 (8) ◽  
pp. 1192-1194 ◽  
Author(s):  
Yoshio Tanaka ◽  
Takao Okamoto ◽  
Toshiyasu Imai ◽  
Takahiro Horinouchi ◽  
Hikaru Tanaka ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Phospholipase C ◽  
Mechanical Activity ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle

Effect of Cromakalim and Pinacidil on 86Rb Efflux from Guinea Pig Urinary Bladder Smooth Muscle

Pharmacology ◽  
1994 ◽  
Vol 49 (3) ◽  
pp. 159-166 ◽  
Author(s):  
Shephali Trivedi ◽  
S. Stetz ◽  
J Leviri ◽  
L. Li ◽  
S. Kau
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle

Inhibitory Effects of Antidepressants on Acetylcholine-Induced Contractions in Isolated Guinea Pig Urinary Bladder Smooth Muscle

Pharmacology ◽  
2016 ◽  
Vol 99 (1-2) ◽  
pp. 89-98 ◽  
Author(s):  
Junji Uno ◽  
Keisuke Obara ◽  
Hiroko Suzuki ◽  
Satomi Miyatani ◽  
Daisuke Chino ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Smooth Muscle ◽  
Inhibitory Effects ◽  
Urinary Bladder Smooth Muscle
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