Fetal Cells in Maternal Blood

1963 ◽  
Vol 56 (7) ◽  
pp. 782-783 ◽  
Author(s):  
WILLIS E. BROWN ◽  
GORDON T. COWLES
Keyword(s):  
2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.


1999 ◽  
Vol 19 (4) ◽  
pp. 323-329 ◽  
Author(s):  
Mieke W. J. C. Jansen ◽  
Marieke von Lindern ◽  
Hartmut Beug ◽  
Helen Brandenburg ◽  
Hajo I. J. Wildschut ◽  
...  

2000 ◽  
Vol 20 (3) ◽  
pp. 257-259 ◽  
Author(s):  
Verena Brombacher ◽  
Vivian Kiefer ◽  
Carolyn Troeger ◽  
Yvan Vial ◽  
Sabine Minderer ◽  
...  

1999 ◽  
Vol 19 (7) ◽  
pp. 648-652 ◽  
Author(s):  
Irene M. de Graaf ◽  
Marja E. Jakobs ◽  
Nico J. Leschot ◽  
Ilya Ravkin ◽  
Simon Goldbard ◽  
...  

2005 ◽  
Vol 20 (2) ◽  
pp. 106-112 ◽  
Author(s):  
Britta Christensen ◽  
John Philip ◽  
Steen Kølvraa ◽  
Lene Lykke-Hansen ◽  
Ilona Hromadnikova ◽  
...  

2006 ◽  
pp. 314-314
Author(s):  
Joe Simpson ◽  
Farideh Bischoff

Sign in / Sign up

Export Citation Format

Share Document