Application of an erythroblast scoring system and DNA polymorphism sequence analysis for the gender-independent assessment of fetal cells in maternal blood

2004 ◽  
Vol 191 (6) ◽  
pp. S47
Author(s):  
Donghyun Cha ◽  
Kiarash Khosrotehrani ◽  
Diana Bianchi ◽  
Kirby Johnson
Keyword(s):  
Sequence Analysis ◽  
Scoring System ◽  
Dna Polymorphism ◽  
Maternal Blood ◽  
Fetal Cells ◽  
Independent Assessment

The utility of an erythroblast scoring system and gender-independent short tandem repeat (STR) analysis for the detection of aneuploid fetal cells in maternal blood

2005 ◽  
Vol 25 (7) ◽  
pp. 586-591 ◽  
Author(s):  
Dong Hyun Cha ◽  
Kiarash Khosrotehrani ◽  
Diana W. Bianchi ◽  
Kirby L. Johnson
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Scoring System ◽  
Maternal Blood ◽  
Str Analysis ◽  
Fetal Cells ◽  
And Gender ◽  
Short Tandem

ROC analysis of an erythroblast morphologic scoring system to improve identification of fetal cells in maternal blood

2004 ◽  
Vol 24 (2) ◽  
pp. 117-120 ◽  
Author(s):  
Dong-Hyun Cha ◽  
Antonio Farina ◽  
Diana W. Bianchi ◽  
Kirby L. Johnson
Keyword(s):  
Scoring System ◽  
Roc Analysis ◽  
Maternal Blood ◽  
Fetal Cells

A simple and sensitive erythroblast scoring system to identify fetal cells in maternal blood

2003 ◽  
Vol 23 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Donghyun Cha ◽  
Brad Hogan ◽  
Ralph M. Bohmer ◽  
Diana W. Bianchi ◽  
Kirby L. Johnson
Keyword(s):  
Scoring System ◽  
Maternal Blood ◽  
Fetal Cells

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

The use ofin vitro expanded erythroid cells in a model system for the isolation of fetal cells from maternal blood

1999 ◽  
Vol 19 (4) ◽  
pp. 323-329 ◽  
Author(s):  
Mieke W. J. C. Jansen ◽  
Marieke von Lindern ◽  
Hartmut Beug ◽  
Helen Brandenburg ◽  
Hajo I. J. Wildschut ◽  
...  
Keyword(s):  
Maternal Blood ◽  
Model System ◽  
Erythroid Cells ◽  
Fetal Cells

Fetal Cells in Maternal Blood

1963 ◽  
Vol 56 (7) ◽  
pp. 782-783 ◽  
Author(s):  
WILLIS E. BROWN ◽  
GORDON T. COWLES
Keyword(s):  
Maternal Blood ◽  
Fetal Cells

Choice of anticoagulant can influence the analysis using fluorescencein situ hybridization of fetal cells enriched from maternal blood

2000 ◽  
Vol 20 (3) ◽  
pp. 257-259 ◽  
Author(s):  
Verena Brombacher ◽  
Vivian Kiefer ◽  
Carolyn Troeger ◽  
Yvan Vial ◽  
Sabine Minderer ◽  
...  
Keyword(s):  
Maternal Blood ◽  
Fluorescencein Situ Hybridization ◽  
Fetal Cells
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