SHORT-TERM STORAGE OF HUMAN LYMPHOCYTES PRIOR TO IN VITRO STIMULATION1,2

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.

Download Full-text

Cryopreservation and short-term storage of human lymphocytes for surface marker analysis Comparison of three methods

Journal of Immunological Methods ◽

10.1016/0022-1759(86)90427-8 ◽

1986 ◽

Vol 93 (1) ◽

pp. 15-18 ◽

Cited By ~ 13

Author(s):

Harry E. Prince ◽

Carolyn D. Lee

Keyword(s):

Human Lymphocytes ◽

Surface Marker ◽

Short Term ◽

Marker Analysis ◽

Short Term Storage ◽

Surface Marker Analysis ◽

Term Storage

Download Full-text

Analysis of IL-1βRelease from Cryopreserved Pooled Lymphocytes in Response to Lipopolysaccharide and Lipoteichoic Acid

BioMed Research International ◽

10.1155/2013/689642 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Sreelekshmi R. Nair ◽

C. S. Geetha ◽

P. V. Mohanan

Keyword(s):

Human Lymphocytes ◽

Lipoteichoic Acid ◽

Gram Negative Bacteria ◽

Healthy Individuals ◽

Elisa Method ◽

Short Term ◽

Long Term Storage ◽

Term Storage

Pyrogens are heterogeneous group of fever-inducing substances derived from Gram-positive and Gram-negative bacteria, fungi, and viruses. They incite immune response by producing endogenous pyrogens such as prostaglandins and other proinflammatory cytokines like IL-1β, IL-6, and TNF-α. The present study was to analyze the influence of cryopreservation in IL-1βrelease, a marker for inflammatory response from human lymphocytes, in response to exogenous pyrogenic stimulants. Lymphocytes isolated from pooled blood of multiple healthy individuals were cryopreserved in DMSO and glycerol for periods of 7, 14, 30, and 60 days and were challenged with LPS and LTAin vitro. The inflammatory cytokine, IL-1βrelease, was measured by ELISA method. It was observed that the release of IL-1βincreases instantaneously after the initiation of incubation and reaches a maximum at 3 to 5 hours and then gradually decreases and gets stabilized for both pyrogens. Moreover it was also observed that the effect of cryoprotectants, DMSO (10%) and glycerol (10%), showed almost similar results for short-term storage, but DMSO-preserved lymphocytes yielded a better viability for long-term storage. Thus, the isolated cryopreserved lymphocytes system can be a promising approach for the total replacement/alteration to animal experimentation for pyrogenicity evaluation.

Download Full-text

In vitro conservation of Mandevilla moricandiana (Apocynaceae): short-term storage and encapsulation–dehydration of nodal segments

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-014-9600-x ◽

2014 ◽

Vol 50 (3) ◽

pp. 326-336 ◽

Cited By ~ 8

Author(s):

Sandra Zorat Cordeiro ◽

Naomi Kato Simas ◽

Anaize Borges Henriques ◽

Alice Sato

Keyword(s):

In Vitro Conservation ◽

Nodal Segments ◽

Short Term ◽

Short Term Storage ◽

Term Storage

Download Full-text

Effect of short-term storage of bovine oocytes in different media and temperatures on the subsequent in vitro embryo development

Theriogenology ◽

10.1016/s0093-691x(98)90570-2 ◽

1998 ◽

Vol 49 (1) ◽

pp. 217 ◽

Cited By ~ 2

Author(s):

J. Schwartz ◽

M.R. Schneider ◽

J.L. Rodrigues ◽

H.-D. Reichenbach

Keyword(s):

Embryo Development ◽

Short Term ◽

Short Term Storage ◽

Bovine Oocytes ◽

Term Storage

Download Full-text

A simple technique for the short-term storage of pollen of a dihaploid-inducing diploid potato clone

The Journal of Agricultural Science ◽

10.1017/s0021859600028446 ◽

1977 ◽

Vol 89 (2) ◽

pp. 511-512 ◽

Cited By ~ 4

Author(s):

M. J. De Maine

Keyword(s):

Simple Technique ◽

Diploid Potato ◽

Short Term ◽

Potato Clone ◽

Short Term Storage ◽

Fresh Pollen ◽

Term Storage

SummaryTwo methods of storing pollen of the dihaploid-inducing Dutch (Wageningen) Solatum phureja clone, IVP 48, are described. Pollination results showed that the functional fertility of one type of stored pollen is similar to that of fresh pollen when used in dihaploid induction crosses on tetraploid potatoes. No obvious relationship was found between the in vitro germination of the pollen samples and their functional fertilities in dihaploid induction crosses.

Download Full-text