Micropropagation and in vitro short-term storage of Globba sherwoodiana W.J. Kress & V. Gowda

2020 ◽  
Keyword(s):  
Short Term ◽  
Short Term Storage ◽  
Term Storage

scholarly journals Short-term storage in vitro and large-scale propagation of grapevine genotypes

2012 ◽  
Vol 47 (3) ◽  
pp. 344-350 ◽  
Author(s):  
Rafael de Carvalho Silva ◽  
Zanderluce Gomes Luis ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rate ◽  
Large Scale ◽  
Culture Medium ◽  
Short Term ◽  
Minimal Growth ◽  
Short Term Storage ◽  
Term Storage ◽  
Storage Temperatures ◽  
Number Of Shoots

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.

A simple technique for the short-term storage of pollen of a dihaploid-inducing diploid potato clone

1977 ◽  
Vol 89 (2) ◽  
pp. 511-512 ◽  
Author(s):  
M. J. De Maine
Keyword(s):  
Simple Technique ◽  
Diploid Potato ◽  
Short Term ◽  
Potato Clone ◽  
Short Term Storage ◽  
Fresh Pollen ◽  
Term Storage

SummaryTwo methods of storing pollen of the dihaploid-inducing Dutch (Wageningen) Solatum phureja clone, IVP 48, are described. Pollination results showed that the functional fertility of one type of stored pollen is similar to that of fresh pollen when used in dihaploid induction crosses on tetraploid potatoes. No obvious relationship was found between the in vitro germination of the pollen samples and their functional fertilities in dihaploid induction crosses.

Short-term storage of in vitro produced bovine embryos

1998 ◽  
Vol 49 (1) ◽  
pp. 249 ◽  
Author(s):  
M.R. Schneider ◽  
J. Schwartz ◽  
H.-D. Reichenbach ◽  
J.L. Rodrigues
Keyword(s):  
Bovine Embryos ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage

Encapsulation for in vitro short-term storage and exchange of ginger (Zingiber officinale Rosc.) germplasm

2010 ◽  
Vol 125 (4) ◽  
pp. 761-766 ◽  
Author(s):  
S. Gopala Sundararaj ◽  
Anuradha Agrawal ◽  
Rishi K. Tyagi
Keyword(s):  
Zingiber Officinale ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage
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