P.119: Evaluation of the Transplant Site Environment in Prevascularized Subcutaneous Islet Transplantation

Although islet transplantation is recognized as a useful cellular replacement therapy for severe diabetes, surgeons face difficulties in islet engraftment. The transplant site is a pivotal factor that influences the engraftment. Although the liver is the current representative site for clinical islet transplantation, it is not the best site because of limitations in immunity, inflammation, and hypoxia. White adipose tissue, including omentum, is recognized as a useful candidate site for islet transplantation. Its effectiveness has been evaluated in not only various basic and translational studies using small and large animals but also in some recent clinical trials. In this review, we attempt to shed light on the characteristics and usefulness of white adipose tissue as a transplant site for islets.

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Gastric submucosa is inferior to the liver as transplant site for autologous islet transplantation in pancreatectomized diabetic Beagles

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/s11596-016-1620-9 ◽

2016 ◽

Vol 36 (4) ◽

pp. 529-533 ◽

Cited By ~ 2

Author(s):

Zhu-zeng Yin ◽

Shu-sen Wang ◽

Qiang Li ◽

Ying Huang ◽

Li Chen ◽

...

Keyword(s):

Islet Transplantation ◽

Autologous Islet Transplantation ◽

Transplant Site

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White Adipose Tissue as a Site for Islet Transplantation

Transplantology ◽

10.3390/transplantology1010006 ◽

2020 ◽

Vol 1 (1) ◽

pp. 55-71

Author(s):

Naoaki Sakata ◽

Gumpei Yoshimatsu ◽

Shohta Kodama

Keyword(s):

Adipose Tissue ◽

Islet Transplantation ◽

White Adipose Tissue ◽

Severe Diabetes ◽

Clinical Islet Transplantation ◽

Translational Studies ◽

Large Animals ◽

A Site ◽

Transplant Site ◽

Shed Light

Although islet transplantation is recognized as a useful cellular replacement therapy for severe diabetes, surgeons face difficulties in islet engraftment. The transplant site is a pivotal factor that influences the engraftment. Although the liver is the current representative site for clinical islet transplantation, it is not the best site because of limitations in immunity, inflammation, and hypoxia. White adipose tissue, including omentum, is recognized as a useful candidate site for islet transplantation. Its effectiveness has been evaluated in not only various basic and translational studies using small and large animals but also in some recent clinical trials. In this review, we attempt to shed light on the characteristics and usefulness of white adipose tissue as a transplant site for islets.

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Improved islet transplantation by bioengineered confined transplant site

Biotechnology and Bioengineering ◽

10.1002/bit.25419 ◽

2015 ◽

Vol 112 (9) ◽

pp. 1-1

Keyword(s):

Islet Transplantation ◽

Transplant Site

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The Non-Immune RIP-kbMouse is a Useful Host for Islet Transplantation, as the Diabetes is Spontaneous, Mild and Predictable

International Journal of Experimental Diabetes Research ◽

10.1080/15604280212530 ◽

2002 ◽

Vol 3 (1) ◽

pp. 37-45 ◽

Cited By ~ 9

Author(s):

Robyn M. Sutherland ◽

Joanne N. Mountford ◽

Janette Allison ◽

Leonard C. Harrison ◽

Andrew M. Lew

Keyword(s):

Islet Transplantation ◽

Disease Onset ◽

Nod Mice ◽

Diabetic Mice ◽

Transplant Recipients ◽

Over Expression ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Chemically Induced ◽

Transplant Site

Chemically-induced diabetic mice and spontaneously diabetic NOD mice have been valuable as recipients for experimental islet transplantation. However, their maintenance often requires parenteral insulin. Diabetogenic chemicals can be cytotoxic to the host’s immune system and to other organs some of which are often used as the transplant site. Procurement of diabetic cohorts in the NOD mouse is problematic due to variability in the age of disease onset. We show that RIP-Kbmice, which spontaneously develop non-immune diabetes due to over-expression of the H-2Kbheavy chain in beta cells, offer many advantages as islet transplant recipients. Diabetes is predictable with a relatively narrow range of onset (4 wk) and blood glucose levels (23.0± 4.0 mmol/l for 39 males at 6 weeks of age). The diabetes is mild enough so that most diabetic mice can be maintained to 40 weeks of age without parenteral insulin. This consistency of diabetes avails that outcomes of intervention can be interpreted with confidence.

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