A Case of Trichogerminoma With Pilomatrical Differentiation and a Unique Immunohistochemical Profile

Abstract Objective.—Collagenous spherulosis of the breast is an uncommon localized pattern of basement membrane material deposition that may be mistaken for atypical proliferations or carcinoma. This report describes 9 cases in which the predominant or exclusive appearance of the spherules was basophilic instead of eosinophilic. Design.—The files of all cases of collagenous spherulosis diagnosed at the Armed Forces Institute of Pathology were reviewed to ascertain the frequency of diagnosis. Results.—Spherulosis with a predominantly basophilic pattern had a histochemical and immunohistochemical profile similar to collagenous spherulosis and was associated with more collagenous-appearing forms in 7 of 9 cases. Review of 81 cases showed that collagenous spherulosis was correctly diagnosed in 15% of referrals and was mistaken for intraductal or invasive carcinoma in 11% of cases. Conclusions.—Mucinous and collagenous patterns appear to be related forms of spherulosis. They are underrecognized by pathologists and maybe mistaken for atypia or malignancy.

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Immunohistochemical evaluation of CD25+ cell expression in the progression of periodontal disease

Brazilian Dental Journal ◽

10.1590/s0103-64402012000400003 ◽

2012 ◽

Vol 23 (4) ◽

pp. 322-327 ◽

Cited By ~ 5

Author(s):

Ruthinéia Diogénes Alves Uchoa Lins ◽

Pollianna Muniz Alves ◽

Gustavo Pina Godoy ◽

Ericka Janine Dantas Silveira ◽

Lélia Maria Guedes Queiroz ◽

...

Keyword(s):

Connective Tissue ◽

Periodontal Disease ◽

Chronic Periodontitis ◽

Cellular Activation ◽

Cellular Density ◽

Cellular Pattern ◽

Immunohistochemical Profile ◽

Cell Expression ◽

Diffuse Distribution

It was assessed the immunohistochemical profile of CD25+ cells in cases of chronic gingivitis (CG) and chronic periodontitis (CP). Immunohistochemistry was carried out using streptoavidin-biotin complex and anti-CD25 antibody in 17 cases of CG and 25 cases of CP. Sixteen cases (94.1%) of CG were immunopositive. CD25 was focally expressed in 50% of the sample and diffusely expressed in 25%. The stained cells were localized not only beneath the epithelium, but also far from it. In relation to the cellular density quantification of CD25+ cells, score ++ was the most common. Concerning CP, all cases were immunopositive. CD25+ cells were expressed in focal or diffuse pattern either close or far from the epithelium. Diffuse distribution of positive cells throughout the connective tissue was seen in 60% of the cases and 32% showed focal or diffuse cellular pattern. Sixteen cases (64%) received score +++. It was identified that CD25+ cells are present in either a focal or a diffuse pattern in connective tissue. Significant differences in the density of cellular immunostaining between CG and CP were found. The greatest density was observed in CP cases, which suggests that the infiltrate of lymphocytes show a higher degree of cellular activation in periodontitis compared with gingivitis.

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