Assessing the Reproducibility of Quantitative In Vivo Confocal Microscopy of Corneal Nerves in Different Corneal Locations

Cornea ◽  
2013 ◽  
Vol 32 (10) ◽  
pp. 1331-1338 ◽  
Author(s):  
Gene Kim ◽  
J. Robinson Singleton ◽  
Mark D. Mifflin ◽  
Kathleen B. Digre ◽  
Michael T. Porzio ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Corneal Nerves

scholarly journals Altered Corneal Nerves in Chinese Thyroid-Associated Ophthalmopathy Patients Observed by In Vivo Confocal Microscopy

2019 ◽  
Vol 25 ◽  
pp. 1024-1031 ◽  
Author(s):  
Lian-Qun Wu ◽  
Pei Mou ◽  
Zi-Yu Chen ◽  
Jin-Wei Cheng ◽  
Qi-Hua Le ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Corneal Nerves ◽  
Thyroid Associated Ophthalmopathy

scholarly journals Evaluation of Corneal Nerves and Dendritic Cells By in Vivo Confocal Microscopy After Descemet’s Membrane Keratoplasty For Bullous Keratopathy

2022 ◽  
Author(s):  
Takahiko Hayashi ◽  
Atsuyuki Ishida ◽  
Akira Kobayashi ◽  
Takefumi Yamaguchi ◽  
Nobuhisa Mizuki ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Bullous Keratopathy ◽  
Dependent Manner ◽  
In Vivo Confocal Microscopy ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Corneal Nerves ◽  
Corneal Nerve ◽  
Number Of Branches

Abstract This study evaluated changes in corneal nerves and the number of dendritic cells (DCs) in corneal basal epithelium following Descemet membrane endothelial keratoplasty (DMEK) surgery for bullous keratopathy (BK). Twenty-three eyes from 16 consecutive patients that underwent DMEK for BK were included. Eyes of age-matched patients that underwent pre-cataract surgery (12 eyes) were used as controls. In vivo confocal microscopy was performed pre- and postoperatively at 6, 12, and 24 months. Corneal nerve length, corneal nerve trunks, number of branches, and the number of DCs were determined. The total corneal nerve length of 1634.7 ± 1389.1 μm /mm2 before surgery was significantly increased in a time-dependent manner to 4485.8 ± 1403.7 μm /mm2, 6949.5 ± 1477.1 μm /mm2, and 9389.2 ± 2302.2 μm /mm2 at 6, 12, and 24 months after DMEK surgery, respectively. The DC density in BK cornea pre- and postoperatively at 6 months was significantly higher than in the controls, and decreased postoperatively at 12 and 24 months and was significantly lower than that at 6 months postoperatively. Thus, our results suggest that DMEK can repair and normalize the corneal environment.

Altered Corneal Nerves in Aqueous Tear Deficiency Viewed by In Vivo Confocal Microscopy

Cornea ◽  
2005 ◽  
Vol 24 (7) ◽  
pp. 818-824 ◽  
Author(s):  
Mei Zhang ◽  
Jiaqi Chen ◽  
Lihui Luo ◽  
Qiguo Xiao ◽  
Mingxia Sun ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Corneal Nerves

scholarly journals In vivo confocal microscopy features and clinicohistological correlation of limbal nerve corpuscles

2019 ◽  
pp. bjophthalmol-2018-313199 ◽  
Author(s):  
Mouhamed Ali Al-Aqaba ◽  
Fady S Anis ◽  
Imran Mohammed ◽  
Anjali Dias Samarawickrama Yapa ◽  
Winfried M Amoaku ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Digital Pathology ◽  
In Vivo Confocal Microscopy ◽  
Eye Bank ◽  
Histological Features ◽  
Corneal Nerves ◽  
Normal Human ◽  
Epithelial Layers ◽  
Whole Mounts

AimsTo describe the in vivo confocal microscopy (IVCM) features of human limbal nerve corpuscles (LNCs) and correlate these with the histological features.MethodsWe examined 40 eyes of 29 healthy living subjects (17 female, 12 male; mean age=47.6) by IVCM. Four limbal quadrants were scanned through all epithelial layers and stroma to identify the LNCs and associated nerves. Ten fresh normal human corneoscleral discs from five deceased patients with a mean age of 67 years and 17 eye-bank corneoscleral rims with a mean age of 57.6 years were stained as whole mounts by the acetylcholinesterase (AChE) method to demonstrate LNCs and corneal nerves. Stained tissue was scanned in multiple layers with the NanoZoomer digital pathology microscope. The in vivo results were correlated to the histological findings.ResultsOn IVCM, LNCs were identified in 65% of the eyes studied and were mainly (84%) located in the inferior or superior limbal regions. They appeared either as bright (hyper-reflective) round or oval single structures within the hyporeflective, relatively acellular fibrous core of the palisades or were clustered in groups, often located anterior to the palisades of Vogt. They measured 36 µm in largest diameter (range 20–56 µm). The in vivo features were consistent with the histology, which showed LNCs as strongly AChE positive round or oval structures.ConclusionThe strong correlation with histology will enable use of IVCM to study LNCs in normal and disease conditions.

scholarly journals Laser Scanning In Vivo Confocal Microscopy of Clear Grafts after Penetrating Keratoplasty

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Dai Wang ◽  
Peng Song ◽  
Shuting Wang ◽  
Dapeng Sun ◽  
Yuexin Wang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Corneal Endothelium ◽  
Laser Scanning ◽  
Penetrating Keratoplasty ◽  
In Vivo Confocal Microscopy ◽  
Chronic Injury ◽  
Corneal Nerves ◽  
Endothelium Cells

Purpose.To evaluate the changes of keratocytes and dendritic cells in the central clear graft by laser scanning in vivo confocal microscopy after penetrating keratoplasty (PK).Methods.Thirty adult subjects receiving PK at Shandong Eye Institute and with clear grafts and no sign of immune rejection after surgery were recruited into this study, and 10 healthy adults were controls. The keratocytes and dendritic cells in the central graft were evaluated by laser scanning confocal microscopy, as well as epithelium cells, keratocytes, corneal endothelium cells, and corneal nerves (especially subepithelial plexus nerves).Results.Median density of subepithelial plexus nerves, keratocyte density in each layer of the stroma, and density of corneal endothelium cells were all lower in clear grafts than in controls. The dendritic cells of five (16.7%) patients were active in Bowman’s membrane and stromal membrane of the graft after PK.Conclusions.Activated dendritic cells and Langerhans cells could be detected in some of the clear grafts, which indicated that the subclinical stress of immune reaction took part in the chronic injury of the clear graft after PK, even when there was no clinical rejection episode.

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