Biological characteristics of fluorescent protein-expressing human hepatocellular carcinoma xenograft model in nude mice

A tumor growth model of human hepatocellular carcinoma HepG2 cells in nude mice was employed to investigate the antitumor activity of the total flavonoids extracted fromArachniodes exilis(TFAE)in vivo. Several biochemical assays including hematoxylin-eosin (HE) staining, immunohistochemistry, and Western blot were performed to elucidate the mechanism of action of total flavonoids extracted fromArachniodes exilis(TFAE). The results showed that TFAE effectively inhibited the tumor growth of hepatocellular carcinoma in nude mice and had no significant effect on body weight, blood system, and functions of liver and kidney. Expression levels of proapoptotic proteins Bax and cleaved caspase-3 remarkably increased while the expressions of Bcl-2, HIF-1α, and VEGF were suppressed by TFAE. These results suggested that the antitumor potential of TFEA was implied by the apoptosis of tumor cells and the inhibition of angiogenesis in tumor tissue.

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Herbal extract “Songyou Yin” inhibits tumor growth and prolongs survival in nude mice bearing human hepatocellular carcinoma xenograft with high metastatic potential

Journal of Cancer Research and Clinical Oncology ◽

10.1007/s00432-009-0566-8 ◽

2009 ◽

Vol 135 (9) ◽

pp. 1245-1255 ◽

Cited By ~ 25

Author(s):

Xiu-Yan Huang ◽

Lu Wang ◽

Zi-Li Huang ◽

Qi Zheng ◽

Qi-Song Li ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Tumor Growth ◽

Nude Mice ◽

Metastatic Potential ◽

Human Hepatocellular Carcinoma ◽

Herbal Extract ◽

High Metastatic Potential ◽

Carcinoma Xenograft

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Establishment and characteristic of an orthotopic implantation model of human hepatocellular carcinoma with Luc-GFP-labeled in nude mice

10.1101/462887 ◽

2018 ◽

Author(s):

Jing-jing Zhang ◽

Min-hua Xu ◽

Jie Wang ◽

Xiao-bao Jin ◽

Yan Ma

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Suspension ◽

Cell Line ◽

Nude Mice ◽

Xenograft Model ◽

Human Hepatocellular Carcinoma ◽

Orthotopic Model ◽

Injection Method ◽

Tumor Fragment

AbstractAimTo construct Luc-GFP-labeled human hepatocellular carcinoma (HCC) cell line with high metastatic potential. And to establish a spontaneous metastasis and conveniently monitored orthotopic model of hepatocellular carcinoma in nude mice. Methods: HCCLM3-Luc-GFP cell line stably expressing luciferase (Luc) and green fluorescent protein (GFP) was constructed by lentivirus transfection. The orthotopic xenograft model was established though cell suspension injection method and tumor fragment implanted method. The growth and metastasis of the tumors were observed by in vivo imaging and pathology. Results: HCCLM3-Luc-GFP, a highly metastatic HCC cell line with GFP expression and Luc activity, was obtained. The tumorigenic rates both of two approaches were 100%, but the lung metastatic rate was higher the former than the latter. Conclusion: The orthotopic model of highly metastatic and Luc-GFP-labeled HCC in nude mice was successfully established by above approaches, called as cell suspension injection method and tumor fragment implanted method, respectively. This study provides a new and effective means to monitor the growth of tumors in vivo and to evaluate the efficacy of anti-metastatic drugs against HCC.

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