scholarly journals Establishment and characteristic of an orthotopic implantation model of human hepatocellular carcinoma with Luc-GFP-labeled in nude mice

2018 ◽  
Author(s):  
Jing-jing Zhang ◽  
Min-hua Xu ◽  
Jie Wang ◽  
Xiao-bao Jin ◽  
Yan Ma
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Suspension ◽  
Cell Line ◽  
Nude Mice ◽  
Xenograft Model ◽  
Human Hepatocellular Carcinoma ◽  
Orthotopic Model ◽  
Injection Method ◽  
Tumor Fragment

AbstractAimTo construct Luc-GFP-labeled human hepatocellular carcinoma (HCC) cell line with high metastatic potential. And to establish a spontaneous metastasis and conveniently monitored orthotopic model of hepatocellular carcinoma in nude mice. Methods: HCCLM3-Luc-GFP cell line stably expressing luciferase (Luc) and green fluorescent protein (GFP) was constructed by lentivirus transfection. The orthotopic xenograft model was established though cell suspension injection method and tumor fragment implanted method. The growth and metastasis of the tumors were observed by in vivo imaging and pathology. Results: HCCLM3-Luc-GFP, a highly metastatic HCC cell line with GFP expression and Luc activity, was obtained. The tumorigenic rates both of two approaches were 100%, but the lung metastatic rate was higher the former than the latter. Conclusion: The orthotopic model of highly metastatic and Luc-GFP-labeled HCC in nude mice was successfully established by above approaches, called as cell suspension injection method and tumor fragment implanted method, respectively. This study provides a new and effective means to monitor the growth of tumors in vivo and to evaluate the efficacy of anti-metastatic drugs against HCC.

scholarly journals Inhibition of Tumor Growth of Human Hepatocellular Carcinoma HepG2 Cells in a Nude Mouse Xenograft Model by the Total Flavonoids fromArachniodes exilis

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Huimin Li ◽  
Dengzhao Jiang ◽  
Lei Zhang ◽  
Jiazhong Wu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Hepg2 Cells ◽  
Xenograft Model ◽  
Human Hepatocellular Carcinoma ◽  
Total Flavonoids ◽  
Tumor Growth Model ◽  
Mouse Xenograft Model

A tumor growth model of human hepatocellular carcinoma HepG2 cells in nude mice was employed to investigate the antitumor activity of the total flavonoids extracted fromArachniodes exilis(TFAE)in vivo. Several biochemical assays including hematoxylin-eosin (HE) staining, immunohistochemistry, and Western blot were performed to elucidate the mechanism of action of total flavonoids extracted fromArachniodes exilis(TFAE). The results showed that TFAE effectively inhibited the tumor growth of hepatocellular carcinoma in nude mice and had no significant effect on body weight, blood system, and functions of liver and kidney. Expression levels of proapoptotic proteins Bax and cleaved caspase-3 remarkably increased while the expressions of Bcl-2, HIF-1α, and VEGF were suppressed by TFAE. These results suggested that the antitumor potential of TFEA was implied by the apoptosis of tumor cells and the inhibition of angiogenesis in tumor tissue.

scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma

scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma

scholarly journals Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2557
Author(s):  
Martina Štampar ◽  
Barbara Breznik ◽  
Metka Filipič ◽  
Bojana Žegura
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Cell Model ◽  
Human Hepatocellular Carcinoma ◽  
Time Dependent ◽  
Hepg2 Cell Line ◽  
Cell Models

In genetic toxicology, there is a trend against the increased use of in vivo models as highlighted by the 3R strategy, thus encouraging the development and implementation of alternative models. Two-dimensional (2D) hepatic cell models, which are generally used for studying the adverse effects of chemicals and consumer products, are prone to giving misleading results. On the other hand, newly developed hepatic three-dimensional (3D) cell models provide an attractive alternative, which, due to improved cell interactions and a higher level of liver-specific functions, including metabolic enzymes, reflect in vivo conditions more accurately. We developed an in vitro 3D cell model from the human hepatocellular carcinoma (HepG2) cell line. The spheroids were cultured under static conditions and characterised by monitoring their growth, morphology, and cell viability during the time of cultivation. A time-dependent suppression of cell division was observed. Cell cycle analysis showed time-dependent accumulation of cells in the G0/G1 phase. Moreover, time-dependent downregulation of proliferation markers was shown at the mRNA level. Genes encoding hepatic markers, metabolic phase I/II enzymes, were time-dependently deregulated compared to monolayers. New knowledge on the characteristics of the 3D cell model is of great importance for its further development and application in the safety assessment of chemicals, food products, and complex mixtures.

Sign in / Sign up

Export Citation Format

Share Document