The absorption of 131 I-labelled serum and colostral proteins from the gut of the young calf

1958 ◽  
Vol 149 (935) ◽  
pp. 184-191 ◽  
Keyword(s):  
Serum Albumin ◽  
Bovine Serum ◽  
Calf Serum ◽  
Electrophoretic Separation ◽  
Young Calf ◽  
Adult Bovine Serum ◽  
Electrophoretic Techniques ◽  
Young Rat

The absorption of 131 I-labelled adult bovine serum and colostral proteins by the gut of the newborn calf has been studied by means of tracer and electrophoretic techniques. The several proteins present in the labelled serum and colostrum appeared to be absorbed with equal facility; there was no evidence that the calf gut showed any selectivity in the absorption of proteins such as is known to occur in the young rat. Electrophoretic separation and measurements of radioactivity of calf serum taken 3, 6 and 20 h after feeding with labelled whole serum indicated that the serum albumin and β -globulin left the circulation at a rate greater than that of the γ -globulins. Three hours after feeding 8 to 16% of the labelled proteins were estimated to be circulating in the plasma. At 20 h 5 to 10% were estimated to be present in the circulation.

survival of bovine embryos frozen in media supplemented with newborn calf serum or bovine serum albumin

1984 ◽  
Vol 21 (1) ◽  
pp. 223 ◽  
Author(s):  
K.R. Bondioli ◽  
C.B. Brunson ◽  
C.R. Looney ◽  
J.M. Massey ◽  
A.B. McGrath ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Calf Serum ◽  
Bovine Embryos ◽  
Newborn Calf Serum

Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

1991 ◽  
Vol 3 (5) ◽  
pp. 601 ◽  
Author(s):  
PA Batt ◽  
DK Gardner ◽  
AW Cameron
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Oxygen Concentration ◽  
Bovine Serum ◽  
Calf Serum ◽  
Cell Number ◽  
The Mean ◽  
Number Of Cells

The effect of oxygen concentration and the source of protein in culture medium on the development of 2- to 4-cell goat embryos in vitro was investigated. Embryos were collected from superovulated Angora-Cashmere-cross goats 48 h after ovulation and cultured for 6 days in synthetic oviduct fluid (SOF) medium under one of two oxygen concentrations (20% or 7%) and in the presence of one of five protein sources; Miles bovine serum albumin (Miles BSA), Commonwealth Serum Laboratory bovine serum albumin (CSL BSA), goat serum (GS), fetal calf serum (FCS) and human serum (HS). In the presence of 20% oxygen the percentage of embryos reaching the expanded and/or hatched blastocyst stage in SOF medium containing Miles BSA was 29%, with a mean cell number per embryo of 28.1 +/- 6.0 (+/- s.e.m.). Use of an oxygen concentration of 7% significantly increased the percentage of embryos reaching this stage (80%, P less than 0.01) and the mean number of cells per embryo (65.3 +/- 8.2, P less than 0.01). The mean number of cells of the early-cleavage-stage embryos was significantly lower when the medium contained CSL BSA, GS or FCS (42.7 +/- 5.6, 29.0 +/- 6.1 and 21.3 +/- 3.2, respectively) than with Miles BSA (92.8 +/- 6.4) or HS (104.8 +/- 17.2) (P less than 0.01). Under 7% oxygen and with Miles BSA or HS, embryos were morphologically comparable to those developed in vivo, but the mean cell numbers in vitro were only approximately half those obtained in vivo.

THE COMBINATION OF HEPARIN AND BOVINE SERUM ALBUMIN

1953 ◽  
Vol 31 (5) ◽  
pp. 394-399
Author(s):  
D. W. Clarke ◽  
F. C. Monkhouse
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Acetate Buffer ◽  
Stable Complex ◽  
Albumin Ratio ◽  
Electrophoretic Techniques

The complexing of heparin and bovine serum albumin in acetate buffer at pH 5.0 was studied by electrophoretic techniques. A stable complex with a heparin : albumin mole ratio of 1 : 2 was found, though there was evidence for the existence of unstable complexes with a higher heparin : albumin ratio.

Sign in / Sign up

Export Citation Format

Share Document