A ratiometric fluorescent probe for sensing hypochlorite in physiological saline, bovine serum albumin and fetal bovine/calf serum

Author(s):  
Shirong Fang ◽  
Lihong Wang ◽  
Yongtian Mei ◽  
Kaibo Zheng
2014 ◽  
Vol 30 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Fengling Song ◽  
Yingying Xue ◽  
Xu Wang ◽  
Jingyun Wang ◽  
Xiaoqing Xiong ◽  
...  

The Analyst ◽  
2011 ◽  
Vol 136 (19) ◽  
pp. 4008 ◽  
Author(s):  
Xiaodan Zeng ◽  
Xiaoling Zhang ◽  
Baocun Zhu ◽  
Hongying Jia ◽  
Yamin Li ◽  
...  

2009 ◽  
Vol 34 (4) ◽  
pp. 101-108 ◽  
Author(s):  
Zhimin Cao ◽  
Clint West ◽  
Carol S. Norton-Wenzel ◽  
Robert Rej ◽  
Faith B. Davis ◽  
...  

2013 ◽  
Vol 34 (7) ◽  
pp. 930-934
Author(s):  
曹世娜 CAO Shi-na ◽  
刘保生 LIU Bao-sheng ◽  
李志云 LI Zhi-yun ◽  
种宝红 CHONG Bao-hong

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.


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