Effect of polyvinyl alcohol, bovine serum albumin fraction V, fetal calf serum and fetal calf serum plus bovine serum albumin fraction V on bovine embryo development

The effects of glutamine, hypotaurine, taurine and premixed solutions of essential amino acids (EAA) and non-essential amino acids (NEAA) on in vitro development of porcine zygotes were evaluated. The effects of refreshing the medium and replacing polyvinyl alcohol (PVA) with bovine serum albumin (BSA) on embryonic development were also investigated. Porcine zygotes produced by in vitro maturation (IVM) and in vitro fertilisation (IVF) were cultured in porcine zygote medium (PZM), as the basal culture medium, for 5 days after IVF. The total number of cells in blastocysts was significantly increased by the addition of 2 mm glutamine to PZM, as was blastocyst yields after supplementation with 0.25 to 4 mm glutamine. Addition of 1.25 to 10 mm hypotaurine to PZM significantly increased blastocyst yields. Addition of 5 mm taurine to PZM significantly increased blastocyst yield, whereas taurine had no effect on blastocyst yield in cultures already containing 5 mm hypotaurine. Adding 1× EAA significantly increased the rate of blastocyst formation compared with no or 2× EAA, whereas 2× NEAA significantly increased the total cell numbers in blastocysts compared with no NEAA. Refreshing the medium at Day 3 had no effect on blastocyst yields, whereas medium change significantly reduced the total cell numbers in blastocysts. Adjusting the amino acid concentrations of a chemically defined medium can improve the developmental competence of porcine embryo.

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survival of bovine embryos frozen in media supplemented with newborn calf serum or bovine serum albumin

Theriogenology ◽

10.1016/0093-691x(84)90323-6 ◽

1984 ◽

Vol 21 (1) ◽

pp. 223 ◽

Cited By ~ 1

Author(s):

K.R. Bondioli ◽

C.B. Brunson ◽

C.R. Looney ◽

J.M. Massey ◽

A.B. McGrath ◽

...

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Calf Serum ◽

Bovine Embryos ◽

Newborn Calf Serum

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Adsorption of bovine serum albumin onto glass powder surfaces coated with polyvinyl alcohol

Journal of Applied Polymer Science ◽

10.1002/1097-4628(20001031)78:5<933::aid-app10>3.0.co;2-g ◽

2000 ◽

Vol 78 (5) ◽

pp. 933-940 ◽

Cited By ~ 19

Author(s):

A. K. Bajpai

Keyword(s):

Bovine Serum Albumin ◽

Polyvinyl Alcohol ◽

Serum Albumin ◽

Bovine Serum ◽

Glass Powder

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Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.641-642.858 ◽

2013 ◽

Vol 641-642 ◽

pp. 858-861 ◽

Cited By ~ 1

Author(s):

Zhi Min Ren ◽

Xi Nie ◽

Sheng Shu Ai

Keyword(s):

Bovine Serum Albumin ◽

Polyvinyl Alcohol ◽

Serum Albumin ◽

Human Serum ◽

Phosphate Buffer ◽

Bovine Serum ◽

Room Temperature ◽

Tween 20 ◽

Blocking Agents

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

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